RESUMO
AIM: Determination of enterotoxigenicity and ability to synthesize TSST-1 in S. aureus strains, isolated from breast milk of women, feeding children with infectious pathology. MATERIALS AND METHODS: 35 S. aureus strains, isolated from breast milk of women feeding children with varying infectious pathology in hospitals and as outpatients were studied for the presence of staphylococci enterotoxins (SE) of types A and B and toxic shock syndrome toxin (TSST-1). Determination of SEA, SEB and TSST-1 was carried out by enzyme immunoassay. RESULTS: Toxins were detected in 94.2% of S. aureus strains. SEB was synthesized by 86.7%, SEA--34.3%, TSST-1--42.8% of S. aureus strains. Toxins were detected with equal frequencies in healthy women and women with inflammatory diseases of breasts. Differences in frequency of colonization of intestines of children receiving breast milk, infected with toxigenic and non-toxigenic staphylococci strains was not detected. CONCLUSION: A high frequency of occurrence of enterotoxins and TSST-1 in S. aureus, isolated from breast milk of the mother during infectious pathology in the child was discovered. Enterotoxigenic strains can be detected in breast milk in healthy women. Study of the role of breast milk, infected with S. aureus, producing SEA, SEB And TSST-1 in development of child pathology is necessary.
Assuntos
Toxinas Bacterianas/biossíntese , Enterotoxinas/biossíntese , Leite Humano/microbiologia , Infecções Estafilocócicas/etiologia , Staphylococcus aureus/patogenicidade , Superantígenos/biossíntese , Toxinas Bacterianas/isolamento & purificação , Aleitamento Materno/efeitos adversos , Portador Sadio , Contagem de Colônia Microbiana , Enterotoxinas/isolamento & purificação , Feminino , Humanos , Técnicas Imunoenzimáticas , Lactente , Intestinos/microbiologia , Masculino , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/patologia , Staphylococcus aureus/isolamento & purificação , Staphylococcus aureus/metabolismo , Superantígenos/isolamento & purificaçãoRESUMO
Premature birth is a serious problem of public health around the world owing to the high frequency of complications and perinatal losses. According to the WHO recommendations by gestation terms the premature birth divides into: super early premature birth (SEPB) in the term of 22-27 weeks, early PB in the term of 28-33 weeks and PB in the term of 34-37 weeks. Preterm born infants make 85%, and in the neonatal mortality structure make more than 55%. It is necessary to consider that in recent years the number of women in the premature birth development risk group has increased, to which patients with a uterus scar, extragenital pathology, supporting reproductive technology treatment pregnancy are related.
