Age-related changes in the reproductive potential of the Queensland fruit fly.

Despite the known negative impacts of aging on the reproductive potential of many insects, Bactrocera tryoni populations show a rapid increase in abundance from early to late spring when the population is composed of predominantly old individuals. While some aspects of how male and female reproductive potential are influenced by age for this species are known, no study investigates lifelong reproductive potential of either sex. We conducted a whole-of-life study in the laboratory to assess the effect of age and mating-partner age on reproductive potential of B. tryoni. The fertility of 70 individual females was directly measured by the number of eggs laid and hatched; while 70 individual males' fertility was assessed indirectly by measuring the hatch rate of eggs laid by a female partner. Half of the males and females had access to a same-age virgin mating partner, while the other half received a prime-age virgin partner (17-19 days old): in both groups mating partners were replaced weekly. Results showed that independent of the age of male mating partner, increasing age significantly reduced the fecundity and fertility of female B. tryoni after a peak at approximately 20 days of age. However, females mated with prime-age males showed higher egg hatch rates during early life than did females mated with a same-age mating partner. As indirectly measured through their partner's egg hatch rate, the fertility of B. tryoni males was also affected by the age of the male and their mating-partner's age. Males mated consistently with a prime-age partner showed an increasing trend in the egg hatch rate of their partner: indirect evidence of increasing fertility in males with increasing age. No such affect was seen when males were mated with a same-age female, possible because of the age-related changes in female fecundity and fertility. While fecundity is greatly reduced in old females, the whole-of-life data shows that the very old flies present in the field at the end of winter are physiologically capable of starting the new season's F1 generation. Beyond getting it begun, old females are unlikely to further contribute to the new season's population as their fecundity does not increase even if mated with a prime-age, new generation male. In contrast, old males, if they have subsequent access to new generation females, have the capacity to help contribute to the rapid spring population growth which is observed in the field.

The responsiveness of Bactrocera jarvisi (Diptera: Tephritidae) to two naturally occurring phenylbutaonids, zingerone and raspberry ketone.

The males of different species of Bactrocera and Zeugodacus fruit flies are commonly attracted to plant-derived phenylpropanoids (e.g. methyl eugenol (ME)) or phenylbutanoids (e.g. raspberry ketone (RK)) but almost never to both. However, one particular plant-derived phenylbutanoid, zingerone (ZN), which possesses an intermediate chemical structure between ME and RK, weakly attracts both ME- and RK-responding fruit fly species. Bactrocera jarvisi, an Australian fruit fly species, is weakly attracted to cue lure (an analogue of RK) but strongly attracted to ZN. Here, we investigated the minimum olfactory threshold and optimum sensitivity of B. jarvisi males to ZN and RK as a function of dose, time and sexual maturation. Our results show that B. jarvisi males had a marked preferential response to ZN, with a much lower olfactory threshold and faster response time to ZN than RK. Probit analysis demonstrated that ZN was at least >1600× more potent than RK as a male attractant to B. jarvisi. Although fruit fly male attraction to the phytochemicals is generally associated with sexual maturity, in B. jarvisi immature males were also attracted to ZN. Our results suggest that B. jarvisi males have a fine-tuned olfactory response to ZN, which appears to play a central role in the chemical ecology of this species.

The mating system of the true fruit fly Bactrocera tryoni and its sister species, Bactrocera neohumeralis.

The frugivorous "true" fruit fly, Bactrocera tryoni (Queensland fruit fly), is presumed to have a nonresourced-based lek mating system. This is largely untested, and contrary data exists to suggest Bactrocera tryoni may have a resource-based mating system focused on fruiting host plants. We tested the mating system of Bactrocera tryoni, and its close sibling Bactrocera neohumeralis, in large field cages using laboratory reared flies. We used observational experiments that allowed us to determine if: (i) mating pairs were aggregated or nonaggregated; (ii) mating system was resource or nonresource based; (iii) flies utilized possible landmarks (tall trees over short) as mate-rendezvous sites; and (iv) males called females from male-dominated leks. We recorded nearly 250 Bactrocera tryoni mating pairs across all experiments, revealing that: (i) mating pairs were aggregated; (ii) mating nearly always occurred in tall trees over short; (iii) mating was nonresource based; and (iv) that males and females arrived at the mate-rendezvous site together with no evidence that males preceded females. Bactrocera neohumeralis copulations were much more infrequent (only 30 mating pairs in total), but for those pairs there was a similar preference for tall trees and no evidence of a resource-based mating system. Some aspects of Bactrocera tryoni mating behavior align with theoretical expectations of a lekking system, but others do not. Until evidence for unequivocal female choice can be provided (as predicted under a true lek), the mating system of Bactrocera tryoni is best described as a nonresource based, aggregation system for which we also have evidence that land-marking may be involved.