The association of thiol/disulphide homeostasis and lipid accumulation index with cardiovascular risk factors in overweight adolescents with polycystic ovary syndrome.

OBJECTIVE: To assess thiol/disulphide homeostasis and lipid accumulation product index, and to determine whether they are associated with increased cardiovascular disease (CVD) risk or not in overweight adolescents with polycystic ovary syndrome (PCOS). DESIGN: Case-control study. SETTING: Education and Research Hospital. PATIENTS: Group 1: 43 overweight+PCOS, Group 2: 45 normal weight+PCOS, Group 3: 27 overweight adolescents and Group 4: 96 age-matched, normal weight healthy controls. INTERVENTIONS: Serum lipid profiles, hormonal parameters and thiol/disulphide homeostasis were measured. Lipid accumulation index (LAP index) and homeostasis model assessment (HOMA-IR) were calculated. MAIN OUTCOME MEASURES: The relation between thiol/disulphide homeostasis and LAP index, and increased CVD risk were evaluated in overweight adolescents with PCOS. RESULTS: Native and total thiol levels were significantly lower in overweight+PCOS adolescents when compared with both normal weight PCOS and control adolescents (P = 0·002). LAP index values were significantly higher in Group 1 when compared separately with the rest of the three groups (P < 0·001). Multivariable logistic regression analysis revealed serum total thiol levels of lower than 405·45 µmol/l were independently associated with increased risk of CVD in overweight PCOS adolescents (OR: 1·019, 95% CI: 1·001-1·036). In addition, a LAP index greater than 21·54 was also associated with increased CVD risk in overweight PCOS adolescents (OR: 1·270, 95% CI: 1·174-1·374). CONCLUSION: In conclusion, we suggest that increased LAP index and decreased total thiol levels may contribute to the increased CVD risk in overweight adolescents with PCOS.

Association Between Level of Hepatitis D Virus RNA at Week 24 of Pegylated Interferon Therapy and Outcome.

BACKGROUND & AIMS: Interferon is the only effective treatment for chronic hepatitis D virus (HDV) infection. No rules have been set for stopping treatment based on viral kinetics. We analyzed data from an international study of hepatitis D treatment to identify factors associated with outcomes of pegylated interferon treatment, with and without adefovir. METHODS: We analyzed data from the Hep-Net-International Delta Hepatitis Intervention Trial on 50 patients with compensated liver disease who tested positive for anti-HDV and HDV RNA. Subjects received pegylated interferon α 2a, with adefovir or placebo, or only adefovir, for 48 weeks. Twenty-four weeks after treatment ended, 41 patients were evaluated for levels of HDV RNA and DNA, liver enzymes, and hepatitis B surface antigen (HBsAg); liver biopsy specimens were analyzed for fibrosis. Response to therapy was defined as end-of-treatment response or post-treatment week 24 virologic response. In both cases virologic response was associated with undetectable HDV RNA levels. Patients with less than a 1 log decrease in HDV RNA at the end of treatment were considered null responders. RESULTS: Based on univariate and multivariate analysis, the level of HDV RNA at week 24 of treatment was associated more strongly with response to therapy than other factors analyzed. The level of HBsAg at week 24 of treatment was associated with a response to therapy only in univariate analysis. Lack of HDV RNA at week 24 of treatment, or end of treatment, identified responders with positive predicted values of 71% and 100%, respectively. At 24 weeks after treatment, a decrease in HDV RNA level of less than 1 log, combined with no decrease in HBsAg level, identified null responders with a positive predictive value of 83%. A decrease in HDV RNA level of more than 2 log at week 24 of treatment identified null responders with a negative predictive value of 95%. CONCLUSIONS: Based on an analysis of data from a large clinical trial, the level of HDV RNA at week 24 of treatment with pegylated interferon, with or without adefovir for 48 weeks, can identify patients who will test negative for HDV RNA 24 weeks after the end of treatment. This information can be used to help physicians manage patients receiving therapy for chronic hepatitis D.

The efficacy of 8% Arginine-CaCO3 applications on dentine hypersensitivity following periodontal therapy: a clinical and scanning electron microscopic study.

OBJECTIVES: Periodontal therapy is one of the etiological factors of dentine hypersensitivity (DH). This study aimed to evaluate the efficacy of %8Arginine-CaCO3 on DH that affects patients after periodontal treatment. STUDY DESIGN: Seventy-one teeth from the volunteers (n=36) with history of DH caused by periodontal therapy were included in this study, and randomly divided into two groups: group-1, who received 8%Arginine-CaCO3 and group-2, who received 1.23%NaF-gel. The clinical indices were recorded at first visit.DH was evaluated by using tactile, air-blast, and thermal stimuli. The subject's response was recorded at baseline, immediately (Day-0) and one month after the application. RESULTS AND CONCLUSIONS: The results were statistically analyzed, and it was found that 8% Arginine-CaCO3 treatment was more effective than 1.23% NaF-gel at time intervals. Sensitivity score differences between the groups were statistically significant at Day-28. The 8% Arginine-CaCO3 group exhibited statistically significant reduction in DH on three stimuli at baseline to Day-28. It was concluded that 8% Arginine-CaCO3 is more effective than 1.23% NaF-gel in reduction of patients' pain.

Histologic and histomorphometric assessment of eggshell-derived bone graft substitutes on bone healing in rats.

OBJECTIVE: The objective of this study was to histologically and histomorphometrically evaluate the efficacy of the new formulations of eggshell-derived calcium carbonate in rats. STUDY DESIGN: The study was conducted on 30 adult male rats. Four standardized and circular intrabony defects were created in the both maxilla and mandibula of each animal. Three different graft materials were prepared as follows: 1) Material A: Eggshell-derived calcium carbonate combined with carrageenan gel, 2) Material B: Eggshell-derived calcium carbonate combined with xanthan gum gel, and 3) Material C: Eggshell-derived calcium carbonate powder. The right mandibular defect sites were grafted with Material A in all animals, and defects on the left were grafted with Material B. Defects on the right side of maxilla were received Material C in all animals, and all left maxillary defects were remained untreated as controls. The animals were sacrificed either postoperatively on the 15th day, postoperatively on the 30th day or postoperatively on the 45th day. Histomorphometric measurements were made of the areas of newly formed bone, necrotic bone, fibrous tissue and residual graft material. RESULTS: Material A exhibited the highest level of osteoid formation followed by Material B and Material C on the 45th day. In terms of osteoid formation, statistically significant differences were observed between graft materials and controls at 45th day compared to 15th and 30th day (p<0.05). CONCLUSIONS: Eggshell-derived graft substitutes in both gel and powder forms are biocompatible materials which may have the potential to enhance the new bone formation. Key words:Bone graft material, bone defects, eggshell, histopathological evaluation, rat.

Validity and reliability study of Turkish version on low vision with quality of life questionnaire.

AIM: To adapt the low vision-related quality of life (LVQOL) instrument into Turkish language and to assess its validity and reliability. METHODS: The study was conducted in 387 patients attending the Centre of Low Vision Rehabilitation, Faculty of Medicine, Ankara University. For statistical analyses, the Spearman's correlation coefficient, Cronbach's alpha coefficient and Confirmatory Factor Analysis (CFA) were used. RESULTS: According to results of CFA, the item in the "Adjustment" subscale because of having the factor loading below 0.40, was excluded from the questionnaire. The reliability of the questionnaire was assessed according to Cronbach's alpha coefficients. The reliability of the "Distance Vision, Mobility, and Lighting" subscale was α=0.863; of the "Adjustment" subscale wasα=0.694; "Reading and Fine Work" wasα=0.791, and "Activities of Daily Living" was α=0.770. So these results indicate that the questionnaire is reliable to measure the vision related quality of life of low-vision patients. The correlations between the subscales were also analyzed, and the correlation between "Adjustment" and "Reading and Fine Work" was found to be the lowest (r(s)=0.336, P<0.001), whereas the strongest correlation was found between the "Reading and Fine Work", and "Activities of Daily Living". Additionally, the "Adjustment" dimension showed the strongest correlation with only "Distance Vision, Mobility, and Lighting" dimension. CONCLUSION: After removing the last item in the second dimension, the Turkish adaptation of all dimensions of the LVQOL has been shown to be reliable, valid and suitable for use in patients with low vision in Turkey.

The effects of digital image enhancement on the detection of vertical root fracture.

AIM: To determine the effects of digital image enhancement on observer ability to detect experimentally induced vertical root fractures (VRF). MATERIAL AND METHODS: A total of 64 extracted human mandibular premolar teeth were used in this study. In 32 teeth, VRFs were created in the bucco-lingual planes by gently tapping with screw-type root-canal pins. The remaining 32 intact teeth served as a control group. Digital images were obtained using a charge coupled device sensor. Three observers separately examined the original and four types of digitally enhanced images (enhanced using sharpness, zoom-in, reverse-contrast, and pseudo-3D functions) at 1-week intervals. All teeth were evaluated using a 5-point scale for the presence/absence of VRF. Evaluations of each image set were repeated 1 month after the initial viewings. Kappa coefficients were calculated to investigate the degree of intra- and inter-observer agreement. The areas under the receiver operating characteristic (ROC) curves (Az values) were calculated using the MedCalc statistical software. ROC values for each image type, observer and viewing were compared using t-tests. A level of alpha = 0.05 was considered significant. RESULTS: Kappa coefficients for intra-observer agreement ranged from 0.304 to 0.679. Inter-observer agreement kappa values ranged from 0.109 to 0.399 for the first reading and from 0.106 to 0.380 for the second reading. Statistical comparisons between Az values for each observer showed no significant differences (P > 0.05) among image types. CONCLUSION: There were no differences in diagnostic outcomes among differently enhanced images in the in vitro detection of VRF.