RESUMO
This study proposes a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of mangiferin in medicinal loranthus shrubs and their mango or non-mango host trees. Mangiferin in samples was extracted with a solution of 40% methanol. Analytical determination was conducted by RP-HPLC with ultraviolet detection at 258 nm. Chromatographic separation was achieved on an Inertsil ODS-SP column (250 × 4.6 mm, 5 µm) by isocratic elution with methanol-0.1% aqueous phosphoric acid (31:69, v/v). Mangiferin contents were 5.04 to 18.95 mg/g in mango trees and 0.44 to 3.72 mg/g in medicinal loranthus parasitized on mango host trees. Mangiferin could not be detected in non-mango trees and their loranthus shrubs. This study indicated that host trees could affect the quality of medicinal loranthus by transporting host-inherent components into loranthus.
Assuntos
Cromatografia de Fase Reversa/métodos , Loranthaceae/química , Mangifera/parasitologia , Plantas Medicinais/química , Xantonas/análise , Cromatografia Líquida de Alta Pressão/métodos , Interações Hospedeiro-Parasita , Limite de Detecção , Modelos Lineares , Extratos Vegetais/química , Reprodutibilidade dos Testes , Xantonas/químicaRESUMO
OBJECTIVE: To study the correlation of 1-deoxynojirimycin (DNJ) between Taxilli Herba parasitized in mulberry and its host-plants. METHOD: The contents of DNJ of Taxilli Herba parasitized in mulberry and non-mulberry were determined by RP-HPLC. DNJ was extracted with 0.05 mol x L(-1) HCl, and then detected by fluorescence detector after derivatized with FMOC-Cl at pH 8.0 with borate buffer. The separation was performed on an Agilent C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase of acetonitrile-0.1% aqueous acetic acid (51: 49) at a flow rate of 1.0 mL x min(-1). The wavelength of fluorescence detector was operated at lambda(EX) = 254 nm and lambda(EM) = 322 nm. RESULT: The linear range of DNJ was 3.72-37.2 mg x L(-1) (r = 0.999 9). The average recovery was 96.42%. The contents of DNJ in mulberry and Taxilli Herba parasitized in mulberry were 1.39-10.16 mg x g(-1) and 0.46-2.72 mg x g(-1), respectively. However, the contents of DNJ could not be detected in Taxilli Herba parasitized in non mulberry and its host-plants. CONCLUSION: As the characteristic constituent of mulberry, DNJ was accumulated in Taxilli Herba This method can be applied to the quality control of Taxilli Herba from mulberry.
