Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Environ Pollut ; 350: 123976, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38657893

RESUMO

The lack of knowledge regarding the extent of microbial contamination in Portuguese fitness centers (FC) puts attendees and athletes at risk for bioaerosol exposure. This study intends to characterize microbial contamination in Portuguese FC by passive sampling methods: electrostatic dust collectors (EDC) (N = 39), settled dust (N = 8), vacuum filters (N = 8), and used cleaning mops (N = 12). The obtained extracts were plated in selective culture media for fungi and bacteria. Filters, EDC, and mop samples' extracts were also screened for antifungal resistance and used for the molecular detection of the selected Aspergillus sections. The detection of mycotoxins was conducted using a high-performance liquid chromatograph (HPLC) system and to determine the cytotoxicity of microbial contaminants recovered by passive sampling, HepG2 (human liver carcinoma) and A549 (human alveolar epithelial) cells were employed. The results reinforce the use of passive sampling methods to identify the most critical areas and identify environmental factors that influence microbial contamination, namely having a swimming pool. The cardio fitness area presented the highest median value of total bacteria (TSA: 9.69 × 102 CFU m-2.day-1) and Gram-negative bacteria (VRBA: 1.23 CFU m-2.day-1), while for fungi it was the open space area, with 1.86 × 101 CFU m-2.day-1. Aspergillus sp. was present in EDC and in filters used to collect settled dust. Reduced azole susceptibility was observed in filters and EDC (on ICZ and VCZ), and in mops (on ICZ). Fumonisin B2 was the only mycotoxin detected and it was present in all sampling matrixes except settled dust. High and moderate cytotoxicity was obtained, suggesting that A549 cells were more sensitive to samples' contaminants. The observed widespread of critical toxigenic fungal species with clinical relevance, such as Aspergillus section Fumigati, as well as Fumonisin B2 emphasizes the importance of frequent and effective cleaning procedures while using shared mops appeared as a vehicle of cross-contamination.


Assuntos
Microbiologia do Ar , Monitoramento Ambiental , Fungos , Portugal , Humanos , Monitoramento Ambiental/métodos , Poluição do Ar em Ambientes Fechados/análise , Poluição do Ar em Ambientes Fechados/estatística & dados numéricos , Micotoxinas/análise , Poeira/análise , Células Hep G2 , Células A549 , Bactérias/isolamento & purificação
2.
Front Public Health ; 11: 1310215, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38089025

RESUMO

Introduction: Exercising on regular basis provides countless health benefits. To ensure the health, well-being and performance of athletes, optimal indoor air quality, regular maintenance and ventilation in sport facilities are essential. Methods: This study assessed the levels of particulate, down to the ultrafine range (PM10, PM2.5, and particle number concentration in size range of 20-1,000 nm, i.e., - PNC20-1000 nm), gaseous pollutants (total volatile organic compounds - TVOCs, CO2, and O3) and comfort parameters (temperature - T, relative humidity - RH) in different functional spaces of health clubs (n = 8), under specific occupancy and ventilation restrictions. Results and Discussion: In all HCs human occupancy resulted in elevated particles (up to 2-3 times than those previously reported), considering mass concentrations (PM10: 1.9-988.5 µg/m3 PM2.5: 1.6-479.3 µg/m3) and number (PNC 1.23 × 103 - 9.14 × 104 #/cm3). Coarse and fine PM indicated a common origin (rs = 0.888-0.909), while PNC showed low-moderate associations with particle mass (rs = 0.264-0.629). In addition, up to twice-higher PM and PNC were detected in cardiofitness & bodybuilding (C&B) areas as these spaces were the most frequented, reinforcing the impacts of occupational activities. In all HCs, TVOCs (0.01-39.67 mg/m3) highly exceeded the existent protection thresholds (1.6-8.9 times) due to the frequent use of cleaning products and disinfectants (2-28 times higher than in previous works). On contrary to PM and PNC, TVOCs were higher (1.1-4.2 times) in studios than in C&B areas, due to the limited ventilations combined with the smaller room areas/volumes. The occupancy restrictions also led to reduced CO2 (122-6,914 mg/m3) than previously observed, with the lowest values in HCs with natural airing. Finally, the specific recommendations for RH and T in sport facilities were largely unmet thus emphasizing the need of proper ventilation procedures in these spaces.


Assuntos
Poluentes Atmosféricos , Poluição do Ar em Ambientes Fechados , Poluentes Ambientais , Academias de Ginástica , Humanos , Poluição do Ar em Ambientes Fechados/análise , Material Particulado/análise , Poluentes Atmosféricos/análise , Gases , Dióxido de Carbono , Monitoramento Ambiental
3.
J Toxicol Environ Health A ; 82(9): 591-602, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31288676

RESUMO

Although regular exercise improves overall well-being, increased physical activity results in enhanced breathing which consequently leads to elevated exposure to a variety of air pollutants producing adverse effects. It is well-known that one of these ambient air contaminants is ultrafine particles (UFP). Thus, this study aimed to (1) examine exposure to particle number concentrations (PNC) in size ranging from N20-1000 nm in different sport environments and (2) estimate the respective inhalation doses across varying activity scenarios based upon the World Health Organization recommendations for physical activity. PNC were continuously monitored (TSI P-Trak™ condensation particle counter) outdoors (Out1-Out2) and indoors (Ind1-Ind2; fitness clubs) over 4 weeks. Outdoor PNC (total median 12 563 # cm-3; means of 20 367 # cm-3 at Out1 and 7 122 # cm-3 at Out2) were approximately 1.6-fold higher than indoors (total median 7 653 # cm-3; means of 11 861 # cm-3 at Ind1 and 14 200 # cm-3 at Ind2). The lowest doses were inhaled during holistic group classes (7.91 × 107-1.87 × 108 # per kg body weight) whereas exercising with mixed cardio and strength training led to approximately 1.8-fold higher levels. In order to optimize the health benefit of exercises, environmental characteristics of the locations at which physical activities are conducted need to be considered.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Monitoramento Ambiental , Exercício Físico , Exposição por Inalação/análise , Material Particulado/análise , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Tamanho da Partícula , Portugal , Esportes/classificação , Adulto Jovem
4.
J Hazard Mater ; 359: 56-66, 2018 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-30014915

RESUMO

Associations between indoor air quality (IAQ) and health in sport practise environments are not well understood due to limited knowledge of magnitude of inhaled pollutants. Thus, this study assessed IAQ in four health clubs (HC1-HC4) and estimated inhaled doses during different types of activities. Gaseous (TVOCs, CO, O3, CO2) and particulate pollutants (PM1, PM4) were continuously collected during 40 days. IAQ was influenced both by human occupancy and the intensity of the performed exercises. Levels of all pollutants were higher when clubs were occupied (p < 0.05) than for vacant periods, with higher medians in main workout areas rather than in spaces/studios for group activities. In all spaces, TVOCs highly exceeded legislative limit (600 µg/m3), even when unoccupied, indicating possible risks for the respective occupants. CO2 levels were well correlated with relative humidity (rs 0.534-0.625) and occupancy due to human exhalation and perspiration during exercising. Clubs with natural ventilations exhibited twice higher PM, with PM1 accounting for 93-96% of PM4; both PM were highly correlated (rs 0.936-0.995) and originated from the same sources. Finally, cardio classes resulted in higher inhalation doses than other types of exercising (1.7-2.6).


Assuntos
Poluição do Ar em Ambientes Fechados/análise , Academias de Ginástica , Exposição por Inalação/análise , Adulto , Poluentes Atmosféricos/análise , Dióxido de Carbono/análise , Monitoramento Ambiental , Exercício Físico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ozônio/análise , Material Particulado/análise , Ventilação , Compostos Orgânicos Voláteis/análise , Adulto Jovem
5.
Environ Pollut ; 233: 180-193, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29073525

RESUMO

Fitness centres (FC) represent a unique indoor microenvironment. Exercising on regular basis provides countless health benefits and improves overall well-being, but if these facilities have poor indoor air quality, the respective exercisers might be subjected to some adverse risks. Considering the limited existent data, this work aimed to evaluate particulate pollution (PM10, PM2.5, and ultrafine particles - UFP) in indoor air of FC and to estimate the respective risks for occupants (both staff and exercising subjects). Sampling was conducted during 40 consecutive days of May-June 2014 in general fitness areas, studios and classrooms (for group activities) of four different fitness centres (FC1-FC4) situated within Oporto metropolitan area, Portugal. The results showed that across the four FC, PM10 ranged between 5 and 1080 µg m-3 with median concentrations (15-43 µg m-3) fulfilling the limit (50 µg m-3) of Portuguese legislation in all FC. PM2.5 (medians 5-37 µg m-3; range 5-777 µg m-3) exceeded thresholds of 25 µg m-3 at some FC, indicating potential risks for the respective occupants; naturally ventilated FC exhibited significantly higher PM ranges (p < 0.05). Similarly, UFPs (range 0.5-88.6 × 103 # cm-3) median concentrations were higher (2-3 times) at FC without controlled ventilation systems. UFP were approximately twice higher (p < 0.05) during the occupied periods (mean of 9.7 × 103vs. 4.8 × 103 # cm-3) with larger temporal variations of UFP levels observed in general fitness areas than in classrooms and studios. Cardio activities (conducted in studios and classrooms) led to approximately twice the UFPs intakes than other types of exercising. These results indicate that even short-term physical activity (or more specifically its intensity) might strongly influence the daily inhalation dose. Finally, women exhibited 1.2 times higher UFPs intake than men thus suggesting the need for future gender-specific studies assessing UFP exposure.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Monitoramento Ambiental , Academias de Ginástica/estatística & dados numéricos , Material Particulado/análise , Poluição do Ar em Ambientes Fechados/estatística & dados numéricos , Poeira , Humanos , Tamanho da Partícula , Portugal , Instituições Acadêmicas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...