RESUMO
Details are given of the preparation of isoflavones derivatised so that they act as haptens in the development of immunoassays for large scale screening for phytoestrogens in biological fluid. A specifically designed new cyclisation reaction allowed the direct conversion of 2-acyloxydeoxybenzoins to the required isoflavones.
RESUMO
A new biflavonoid-C-glycoside named occidentoside, also the known (-)salipurposide and beta-sitosterol, have been isolated from the nut shells of Anacardium occidentale L. (Anacardiaceae). The structure of occidentoside has been established as tetrahydroninokiflavone-C-glucoside (III) from spectral and chemical evidence. This is the first biflavonoid to occur with one flavanone and one chalcone unit and also the first C-glycoside in the biflavonoid series. Identification of (-)salipurposide is also reported since it has been isolated for the first time from cashew nut shells.
RESUMO
1. The photodynamic activity of bonellin, an integumentary chlorin of Bonellia viridis, is investigated. 2. 10(-6) M bonellin solutions haemolyze erythrocytes only in the presence of light. Previous illumination (tungsten lamp 2000-4000 lux for 1 hr) of the bonellin solutions does not affect the results. Under lowered oxygen tensions the bioactivity is depressed. Benzoquinone and singlet oxygen quenchers delay the photodynamic effect. 3. Bonellin (2 x 10(-6) M) destroys echinoid gamete function, depresses oxygen uptake of spermatozoa, and arrests development of echinoid and Bonellia eggs. These effects are produced only in the presence of light. 4. Copper bonellin is not photodynamically active. The role of copper may be to protect against photosensitization.
