The Mechanism of Wnt Pathway Regulated by Telocytes to Promote the Regeneration and Repair of Intrauterine Adhesions.

Background: Telocytes (TCs), a novel interstitial cell type in the reproductive tract, participating in pathophysiology of intrauterine adhesions (IUA). This study further investigates the hypothesis that TCs, a source of Wnt, promote the regeneration and repair of IUA. Methods: RNA sequencing datasets of IUA patient (GSE160633) and mouse intestine mesenchymal cells (GSE94072) in GEO database were analyzed for differentially expressed genes (DEGs), and quantitative real-time PCR (qRT-PCR) measured indicated gene expression in TC-educated endometrial stromal cells (ESCs) and noneducated ESCs and verified the results of data mining from GEO database. Results: The expression levels of Wnt genes were downregulated in IUA compared to the control and were upregulated in TCs. In particular, the changes of Wnt5a expression level were the most significant (logFC = 4.0314 and adjusted P value = 0.0023), and the relative Wnt5a expression level was remarkably higher in TC-educated ESCs than noneducated ESCs verified by qRT-PCR (P = 0.0027). Conclusions: TCs may enhance the regeneration and repair of IUA through the Wnt signaling pathway.

[Effects of no-tillage on soil water content and physical properties of spring corn fields in semiarid region of northern China].

Field experiments were conducted in 2006-2008 to study the effects of no-tillage on the spatiotemporal dynamics of soil water content and related soil physical properties in spring corn fields in Beijing region during growth season. In study period, the water storage in 0-100 cm soil layer in tillage and no-tillage treatments had the same variation trend with time and precipitation, but the water storage at different time periods and under different precipitations was 2.7%-30.3% higher in no-tillage treatment than in tillage treatment. When the precipitation was relatively abundant, the increment of soil water storage was somewhat increased, but no-tillage was still worth to be popularized in the regions relatively deficit in precipitation. Under no-tillage, the average water storage in 0-100 cm soil layer during the three growth seasons in 2006-2008 was 3.4%-12.8% higher than that under conventional tillage, and the increment of the water storage in 0-20 cm and 80-100 cm soil layers under no-tillage was higher than that in intermediate layer, with the highest increment reached 22.2%. No-tillage improved soil water-holding capacity and water use efficiency via decreasing soil bulk density, increasing soil porosity, and promoting the formation of soil water-stable aggregates, and thereby, promoted crop yielding. After 3 years no-tillage, the soil water use efficiency and spring corn yield were increased by 13.3% and 16.4%, respectively, compared with those under conventional tillage.