Removal, transformation and ecological risk assessment of pesticide in rural wastewater by field-scale horizontal flow constructed wetlands of treated effluent.

Constructed wetlands (CWs) are widely used in sewage treatment in rural areas, but there are only a few studies on field-scale CWs in treating wastewater-borne pesticides. In this study, the treatment and metabolic transformation of 29 pesticides in rural domestic sewage by 10 field-scale horizontal flow CWs (HF-CWs), each with a treatment scale of 36‒5000 m3/d and operated for 2‒10 years, in Guangzhou, Southern China was investigated. The risk of pesticides in treated effluent and main factors influencing such risk were evaluated. Results demonstrated that HF-CWs could remove pesticides in sewage and reduce their ecological risk in effluent, but the degree varied among types of pesticides. Herbicides had the highest mean removal rate (67.35 %) followed by insecticides (60.13 %), and the least was fungicides (53.22 %). In terms of single pesticide compounds, the mean removal rate of butachlor was the highest (73.32 %), then acetochlor (69.41 %), atrazine (68.28 %), metolachlor (58.40 %), and oxadixyl (53.28 %). The overall removal rates of targeted pesticides in each HF-CWs ranged from 11 %‒57 %, excluding two HF-CWs showing increases in pesticides in treated effluent. Residues of malathion, phorate, and endosulfan in effluent had high-risks (RQ > 5). The pesticide concentration in effluent was mainly affected by that in influent (P = 0.042), and source control was the key to reducing risk. The main metabolic pathways of pesticide in HF-CWs were oxidation, with hydroxyl group to carbonyl group or to form sulfones, the second pathways by hydrolysis, aerobic condition was conducive to the transformation of pesticides. Sulfones were generally more toxic than the metabolites produced by hydrolytic pathways. The present study provides a reference on pesticides for the purification performance improvement, long-term maintenance, and practical sustainable application of field-scale HF-CWs.

The pictorial set of Emotional Social Interactive Scenarios between Chinese Adults (ESISCA): Development and validation.

Affective picture databases with a single facial expression or body posture in one image have been widely applied to investigate emotion. However, to date, there was no standardized database containing the stimuli which involve multiple emotional signals in social interactive scenarios. The current study thus developed a pictorial set comprising 274 images depicting two Chinese adults' interactive scenarios conveying emotions of happiness, anger, sadness, fear, disgust, and neutral. The data of the valence and arousal ratings of the scenes and the emotional categories of the scenes and the faces in the images were provided in the present study. Analyses of the data collected from 70 undergraduate students suggested high reliabilities of the valence and arousal ratings of the scenes and high judgmental agreements in categorizing the scene and facial emotions. The findings suggested that the present dataset is well constructed and could be useful for future studies to investigate the emotion recognition or empathy in social interactions in both healthy and clinical (e.g., ASD) populations.

Theory and Meditation of Confucian Mindfulness: Analysis Based on the Concept of Vigilance in Solitude in Chinese Confucianism.

Mindfulness has become an area of great interest in Western psychology, but it has been criticized for its de-religionization. Based on Chinese Confucianist Liu Zongzhou's concept of shendu (; vigilance in solitude), this study attempts to present a complete overview of the Confucian mindfulness meditation system from the theoretical basis and training method. The unique value of Confucian mindfulness meditation is that it directly points to morality, which can make up for the deficiency of mindfulness in Western psychology. Furthermore, Confucian mindfulness meditation can provide more suitable training methods for individuals who are culturally Chinese.

Om chanting modulates the processing of negative stimuli: Behavioral and electrophysiological evidence.

Previous studies have suggested that Om chanting, a type of meditation, can relieve individuals' negative emotions. However, the dynamic aspects of neural processes in the processing of the negative stimuli while Om chanting are still unclear. In this study, we recruited 33 healthy undergraduate students without meditation experience and recorded event-related potentials (ERPs) to unpleasant and neutral images when they performed Om chanting and viewing task. The behavioral results showed that the unpleasant images were rated as less unpleasant and arousing in the condition of Om chanting than while passive viewing, and the rates were not different between the two conditions for the neutral images. Analyses of the ERP responses to the emotional stimuli revealed that Om chanting decreased P1 and late positive potential (LPP) amplitudes for the neutral images but not for the unpleasant images. We speculated that Om chanting might reduce vigilance to the neutral stimuli, whereas for the negative stimuli, they automatically captured all available attentional resources and led to a failure in observing the regulating effect of Om chanting. These observations suggest that Om chanting modulates individuals' affective evaluations to the negative stimuli and alters early visual and late neural processing of the stimuli.

Shikonin attenuates kidney tubular epithelial cells apoptosis, oxidative stress, and inflammatory response through nicotinamide adenine dinucleotide phosphate oxidase 4/PTEN pathway in acute kidney injury of sepsis model.

Natural compounds were used in the treatment of acute kidney injury (AKI) caused by sepsis. This study investigated the function of shikonin from the roots of Arnebia purpurea in sepsis-induced AKI model. The target genes of shikonin were predicted by traditional Chinese medicine integrative database (TCMID). The markers of kidney injury, oxidative stress, and inflammatory factors were measured by enzyme-linked immunosorbent assay (ELISA). The pathological changes of kidney tubules were assessed by Hematoxylin and Eosin staining. Apoptosis of kidney tubular epithelial cells (KTECs) was detected by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Protein expression was measured by western blot. Shikonin significantly improved kidney injury induced by cecal ligation and perforation (CLP). Besides, shikonin reduced KTECs apoptosis, malondialdehyde (MDA), reactive oxygen species (ROS), interleukin-1ß (IL-1ß), IL-6, and tumor necrosis factor-α (TNF-α) levels, while augmented SOD and IL-10 levels. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase4 (NOX4) was predicted a target gene of shikonin. The expression of NOX4 was significantly inhibited in shikonin-treated group and the levels of phosphatidylinositol 3,4,5-trisphosphate 3-phosphate and dual specificity protein phosphate (PTEN) and p-p65 were decreased, while level of p-Akt was elevated. In vitro experiments, shikonin inhibited cell apoptosis, inflammatory, and ROS in human HK-2 cells and rat TECs. Shikonin downregulated expression of NOX4, PTEN and p-p65, and upregulated p-AKT and Bcl-2 expression in HK2 cells treated with lipopolysaccharide (LPS). Moreover, overexpression of NOX4 enhanced the effect of LPS on the expression level of PTEN, p-p65, p-AKT, and Bcl-2, which was reversed by the addition of shikonin. Taken together, shikonin could improve sepsis-induced AKI in rats, and attenuate the LPS induced KTECs apoptosis, oxidative stress, and inflammatory reaction via modulating NOX4/PTEN/AKT pathway.