Fuzheng Kang' ai decoction inhibits cell proliferation, migration and invasion by modulating mir-21-5p/human phosphatase and tensin homology deleted on chromosome ten in lung cancer cells.

OBJECTIVE: To elucidate the potential molecular mechanism by which Fuzheng Kang'ai decoction (, FZKA) inhibits proliferation, migration, and invasion of lung cancer cells. METHODS: Varying FZKA concentrations were used to manage lung cancer cells (A549 and PC9). We employed: cell counting kit-8 (CCK-8) and plate clone for-mation assays to examine the cell viability; flow cytometry (FCM) to analyze the cycle arrest; transwell and wound-healing assays to assess the cell invasion and migration, respectively. Further, a quantitative real-time polymerase chain reaction (qRT-PCR) assay was adopted to evaluate the miR-21-5p expression. For protein expression analysis, we employed the Western blot technique. Recombinant miR-21-5p overexpression adenovirus vector harboring GFP was constructed and transfected into A549 and PC9, after which we explored the effect of FZKA on miR-21-5p overexpression. RESULTS: Notably, treatment with FZKA inhibited viability, clone-formation ability, invasion, and migration of lung cancer cells. Mechanistically, FZKA markedly suppressed miR-21-5p expression but elevated the human phosphatase and tensin homology deleted on chromosome ten (PTEN) protein level in both A549 and PC9 cells. Over-expression of miR-21-5p lowered PTEN protein expression. Besides, overexpressed miR-21-5p levels with adenovirus antagonized FZKA-upregulated PTEN protein expression. CONCLUSION: The present study demonstrates how FZKA modulates cell biological behaviors, for instance, it impedes the proliferation by upregulating PTEN expression with miR-21-5p as the target. These findings unveil the potential novel molecular mechanisms from the microRNA aspect by which FZKA suppresses the growth of human lung cancer cells.

Evaluation the changes of function of right ventricular using Echocardiography after transcatheter closure of atrial septal defect / 实用医学杂志

Objective To evaluate the changes of function of right ventricular using echocardiography after transcatheter closure of atrial septal defect(ASD)and to study the feasibility and superiority of echocardiographic evaluation of right ventricular function. Methods In 70 patients with transcatheter closure of ASD,echocardio?graphic examinations were made different time intervals after the closure to calculate right cardiac morpnology and function. Results After closure ASD in different time intervals, the size of RAEDd1, RAEDd2, RVEDd1, RVEDd2, RVEDd3, Inferior Vena Cava and RIMP, RVEF, TAPSE and FAC were obviously decreased(P<0.01)between two groups. All events were obviously decreased compared precious function(P < 0.01)and the interaction of the time (P < 0.01). Conclusion The construction of right ventricular narrows gradually and the function recovers after transcatheter closure of ASD in a year and those who did not become worse.