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1.
Chem Biol ; 14(4): 431-41, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17462578

RESUMO

High-mobility group box 1 protein (HMGB1) is a nuclear component, but extracellularly it serves as a signaling molecule involved in acute and chronic inflammation, for example in sepsis and arthritis. The identification of HMGB1 inhibitors is therefore of significant experimental and clinical interest. We show that glycyrrhizin, a natural anti-inflammatory and antiviral triterpene in clinical use, inhibits HMGB1 chemoattractant and mitogenic activities, and has a weak inhibitory effect on its intranuclear DNA-binding function. NMR and fluorescence studies indicate that glycyrrhizin binds directly to HMGB1 (K(d) approximately 150 microM), interacting with two shallow concave surfaces formed by the two arms of both HMG boxes. Our results explain in part the anti-inflammatory properties of glycyrrhizin, and might direct the design of new derivatives with improved HMGB1-binding properties.


Assuntos
Anti-Inflamatórios não Esteroides/metabolismo , Citocinas/antagonistas & inibidores , Ácido Glicirrízico/metabolismo , Proteína HMGB1/antagonistas & inibidores , Proteína HMGB1/metabolismo , Células 3T3 , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Sítios de Ligação , Citocinas/metabolismo , DNA/metabolismo , Fluorescência , Ácido Glicirrízico/química , Ácido Glicirrízico/farmacologia , Proteína HMGB1/química , Humanos , Espectroscopia de Ressonância Magnética , Camundongos , Mitógenos/antagonistas & inibidores , Mitógenos/farmacologia , Modelos Moleculares , Ligação Proteica
2.
J Biol Chem ; 280(12): 11505-12, 2005 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-15649886

RESUMO

Mutations in the autoimmune regulator protein AIRE1 cause a monogenic autosomal recessively inherited disease: autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). AIRE1 is a multidomain protein that harbors two plant homeodomain (PHD)-type zinc fingers. The first PHD finger of AIRE1 is a mutational hot spot, to which several pathological point mutations have been mapped. Using heteronuclear NMR spectroscopy, we determined the solution structure of the first PHD finger of AIRE1 (AIRE1-PHD1), and characterized the peptide backbone mobility of the domain. We performed a conformational analysis of pathological AIRE1-PHD1 mutants that allowed us to rationalize the structural impact of APECED-causing mutations and to identify an interaction site with putative protein ligands of the AIRE1-PHD1 domain. The structure unequivocally exhibits the canonical PHD finger fold, with a highly conserved tryptophan buried inside the structure. The PHD finger is stabilized by two zinc ions coordinated in an interleaved (cross-brace) scheme. This zinc coordination resembles RING finger domains, which can function as E3 ligases in the ubiquitination pathway. Based on this fold similarity, it has been suggested that PHD fingers might also function as E3 ligases, although this hypothesis is controversial. At variance to a previous report, we could not find any evidence that AIRE1-PHD1 has an intrinsic E3 ubiquitin ligase activity, nor detect any direct interaction between AIRE1-PHD1 and its putative cognate E2. Consistently, we show that the AIRE1-PHD1 structure is clearly distinct from the RING finger fold. Our results point to a function of the AIRE1-PHD1 domain in protein-protein interactions, which is impaired in some APECED mutations.


Assuntos
Candidíase/genética , Displasia Ectodérmica/genética , Mutação , Poliendocrinopatias Autoimunes/genética , Fatores de Transcrição/química , Dedos de Zinco , Sequência de Aminoácidos , Humanos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Dobramento de Proteína , Fatores de Transcrição/fisiologia , Ubiquitina-Proteína Ligases/metabolismo , Proteína AIRE
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