Influence of seasonality and biological activity on infection by helminths in Cantabrian bear.

This study aimed to investigate the variations of parasites in the feces of brown bears Ursus arctos inhabiting the Cantabrian Mountains (NW Spain). A total of 248 bear fecal samples were collected throughout one year, spanning from August 2018 to September 2019, at an approximate frequency of 20 samples per month. The results were analyzed in relation to both the season and the biological activity of the brown bears, i.e., hibernation, mating and hyperphagia. Among the examined samples, eggs of Dicrocoelium dendriticum (32.2%; 95% Confidence Interval: 26.4-38.1), Baylisascaris sp. (44.8%; 38.5-50.9), ancylostomatids (probably belonging to Uncinaria spp.) (16.5%; 11.9-21.1) and Trichuris sp. (1.2%; 0-2.6) were observed. Significant seasonal differences were noted for Baylisascaris and ancylostomatids (χ2 = 21.02, P = 0.001 and χ2 = 34.41, P = 0.001, respectively). Furthermore, the presence of helminth eggs was correlated with the activity phase of the brown bears. Dicrocoelium attained the highest prevalence during the mating phase, while Baylisascaris and ancylostomatids were more frequent during hyperphagia. Notably, the highest egg-output counts for Dicrocoelium and Baylisascaris sp. were recorded during the mating phase and hibernation, respectively, whereas ancylostomatids eggs peaked during hyperphagia. Additionally, variations in egg-output counts were significant for all helminths concerning the season, with the exception of Trichuris sp., and for Dicrocoelium and Baylisascaris sp. According to bear activity. It is concluded that infection by gastrointestinal helminths depends on the season and the biological activity of the bears from the Cantabrian Mountains, and their health status could result influenced.

Sex-, age-, and time-specific visual communication in brown bears.

Intraspecific communication in mammals is well-documented but generally restricted to chemical and acoustic signaling. However, other overlooked channels, such as visual signaling, may be used to communicate among conspecifics. Here, by using experimental manipulations together with camera traps on 13 brown bear (Ursus arctos) rubbing trees in the Cantabrian Mountains (northwestern Spain), we document detailed temporal patterns and behavioral aspects of a recently discovered novel communication channel for this species, visual signaling through the trunk debarking of focal trees. Video footage showed that visual marking is a sex-, age-, and time-specific means of communication in brown bears, being performed exclusively by adult males during the mating season (mainly April-June in the study area). Trunk debarking was always associated with chemical marking and was never an isolated behavior, suggesting that visual and chemical signals might be complementary. Visual and chemical marks may provide different information; for example, visual marks could be an indicator of individual size and, thus, the dominance status of adult males looking for mating opportunities. This is the first time that evidence is provided showing that visual signaling in a large carnivore is exclusive to a specific class of individuals (adult males) and linked to reproductive needs only. Bear visual signaling not only represents an advance in our comprehension of animal communication but may also serve to easily locate the mating areas of mammals, which are crucial for large carnivore species, such as the brown bear, that frequently need specific and urgent plans for conservation and management.

La comunicación intraespecífica en los mamíferos está bien documentada, pero generalmente se limita a la señalización química y acústica. Sin embargo, otros canales ignorados, como la señalización visual, pueden ser utilizados para comunicarse entre conespecíficos. Aquí, utilizando manipulaciones experimentales junto con cámaras trampa en trece árboles de marcaje de oso pardo Ursus arctos en la Cordillera Cantábrica (noroeste de España), documentamos patrones temporales detallados y aspectos conductuales de un nuevo canal de comunicación recientemente descubierto para esta especie, es decir, la señalización visual a través del descortezado del tronco de árboles focales. Las imágenes de vídeo mostraron que el marcado visual es un medio de comunicación específico de sexo, edad y tiempo en los osos pardos, siendo realizado exclusivamente por los machos adultos durante la época de celo (principalmente entre abril y junio en el área de estudio). El descortezado del tronco siempre estuvo asociado al marcaje químico, y nunca fue un comportamiento aislado, lo que sugiere que las señales visuales y químicas podrían ser complementarias. Las marcas visuales pueden proporcionar una información diferente a las químicas, como por ejemplo el tamaño del individuo y, por tanto, el estatus de dominancia de los machos adultos en busca de oportunidades de apareamiento. Es la primera vez que se aportan pruebas que demuestran que la señalización visual en un gran carnívoro es exclusiva de una clase específica de individuos (los machos adultos) y está vinculada únicamente a las necesidades reproductivas. La señalización visual de los osos no sólo representa un avance en nuestra comprensión de la comunicación animal, sino que también puede servir para localizar fácilmente las zonas de celo de los mamíferos, lo cual es crucial para las especies de grandes carnívoros, como el oso pardo, que con frecuencia necesitan planes específicos y urgentes de conservación y gestión.

The Different Fate of the Pyrenean Desman (Galemys pyrenaicus) and the Eurasian Otter (Lutra lutra) under Climate and Land Use Changes.

Climate and land use change can affect biodiversity in different ways, e.g., determining habitat loss, altering reproduction periods or disrupting biotic interactions. Here, we investigate the effects of climate and land use change on the spatial distribution of two semi-aquatic mammals, the Pyrenean desman (Galemys pyrenaicus) and the Eurasian otter (Lutra lutra). We first modeled the current potential distribution of the desman and the otter in the Iberian Peninsula, considering topographic, climatic and land use variables. Second, we predicted their potential distribution in 2050 under climate and land use change scenarios. We calculated the percentage of range gain/loss and shift predicted for the two species under such scenarios and quantified the present and future spatial overlap between the two species distribution. Irrespective of the scenario, desman models show loss of suitable habitat, whereas the otter will undergo an opposite trend. Aside from a preponderant habitat loss, the desman is predicted to increase its spatial overlap with otter range under the optimistic scenarios, potentially meaning it will face an exacerbated predation by otters. The potential increase of both habitat loss and otters' predation might represent a major threat for the desman, which may affect the long-term persistence of this endemic species in the Iberian Peninsula.

A worldwide perspective on large carnivore attacks on humans.

Large carnivores have long fascinated human societies and have profound influences on ecosystems. However, their conservation represents one of the greatest challenges of our time, particularly where attacks on humans occur. Where human recreational and/or livelihood activities overlap with large carnivore ranges, conflicts can become particularly serious. Two different scenarios are responsible for such overlap: In some regions of the world, increasing human populations lead to extended encroachment into large carnivore ranges, which are subject to increasing contraction, fragmentation, and degradation. In other regions, human and large carnivore populations are expanding, thus exacerbating conflicts, especially in those areas where these species were extirpated and are now returning. We thus face the problem of learning how to live with species that can pose serious threats to humans. We collected a total of 5,440 large carnivore (Felidae, Canidae, and Ursidae; 12 species) attacks worldwide between 1950 and 2019. The number of reported attacks increased over time, especially in lower-income countries. Most attacks (68%) resulted in human injuries, whereas 32% were fatal. Although attack scenarios varied greatly within and among species, as well as in different areas of the world, factors triggering large carnivore attacks on humans largely depend on the socioeconomic context, with people being at risk mainly during recreational activities in high-income countries and during livelihood activities in low-income countries. The specific combination of local socioeconomic and ecological factors is thus a risky mix triggering large carnivore attacks on humans, whose circumstances and frequencies cannot only be ascribed to the animal species. This also implies that effective measures to reduce large carnivore attacks must also consider the diverse local ecological and social contexts.

Modeling climate change impacts on the distribution of an endangered brown bear population in its critical habitat in Iran.

Climate change is one of the major challenges to the current conservation of biodiversity. Here, by using the brown bear, Ursus arctos, in the southernmost limit of its global distribution as a model species, we assessed the impact of climate change on the species distribution in western Iran. The mountainous forests of Iran are inhabited by small and isolated populations of brown bears that are prone to extinction in the near future. We modeled the potential impact of climate change on brown bear distribution and habitat connectivity by the years 2050 and 2070 under four representative concentration pathways (RCPs) of two general circulation models (GCMs): BCC-CSM1-1 and MRI-CGCM3. Our projections revealed that the current species' range, which encompasses 6749.8 km2 (40.8%) of the landscape, will decline by 10% (2050: RCP2.6, MRI-CGCM3) to 45% (2070: RCP8.5, BCC-CSM1-1). About 1850 km2 (27.4%) of the current range is covered by a network of conservation (CAs) and no-hunting (NHAs) areas which are predicted to decline by 0.64% (2050: RCP2.6, MRI-CGCM3) to 15.56% (2070: RCP8.5, BCC-CSM1-1) due to climate change. The loss of suitable habitats falling within the network of CAs and NHAs is a conservation challenge for brown bears because it may lead to bears moving outside the CAs and NHAs and result in subsequent increases in the levels of bear-human conflict. Thus, re-evaluation of the network of CAs and NHAs, establishing more protected areas in suitable landscapes, and conserving vital linkages between habitat patches under future climate change scenarios are crucial strategies to conserve and manage endangered populations of the brown bear.

Rubbing behavior of European brown bears: factors affecting rub tree selectivity and density.

Scent-mediated communication is considered the principal communication channel in many mammal species. Compared with visual and vocal communication, odors persist for a longer time, enabling individuals to interact without being in the same place at the same time. The brown bear (Ursus arctos), like other mammals, carries out chemical communication, for example, by means of scents deposited on marking (or rub) trees. In this study, we assessed rub tree selectivity of the brown bear in the predominantly deciduous forests of the Cantabrian Mountains (NW Spain). We first compared the characteristics of 101 brown bear rub trees with 263 control trees. We then analyzed the potential factors affecting the density of rub trees along 35 survey routes along footpaths. We hypothesized that: (1) bears would select particular trees, or tree species, with characteristics that make them more conspicuous; and (2) that bears would select trees located in areas with the highest presence of conspecifics, depending on the population density or the position of the trees within the species' range. We used linear models and generalized additive models to test these hypotheses. Our results showed that brown bears generally selected more conspicuous trees with a preference for birches (Betula spp.). This choice may facilitate the marking and/or detection of chemical signals and, therefore, the effectiveness of intraspecific communication. Conversely, the abundance of rub trees along footpaths did not seem to depend on the density of bear observations or their relative position within the population center or its border. Our results suggest that Cantabrian brown bears select trees based on their individual characteristics and their location, with no influence of characteristics of the bear population itself. Our findings can be used to locate target trees that could help in population monitoring.

La comunicación olfativa se considera el principal canal de comunicación en muchas especies de mamíferos. En comparación con la comunicación visual y la vocal, los olores persisten durante más tiempo, lo que permite a los individuos interactuar sin estar en el mismo lugar al mismo tiempo. El oso pardo (Ursus arctos), al igual que otros mamíferos, emplea la comunicación química, por ejemplo, por medio de olores depositados en árboles a través del marcaje o rascado. En este estudio, evaluamos la selección de árboles de marcaje por el oso pardo en los bosques predominantemente caducifolios de la Cordillera Cantábrica (noroeste de España). En primer lugar, comparamos las características individuales de 101 árboles de marcaje de oso pardo con 263 árboles control. Después, analizamos los factores potenciales que afectan la densidad de árboles de marcaje en 35 trayectos de prospección a lo largo de caminos y pistas forestales. Planteamos las hipótesis que: (1) los osos seleccionan árboles particulares, o especies de árboles, con características que los hacen más conspicuos; y (2) que los osos seleccionan árboles ubicados en áreas con mayor presencia de conespecíficos, dependiendo de la densidad de población osera o de la posición de los árboles dentro del rango de distribución de la especie. Usamos modelos lineales y modelos aditivos generalizados para probar estas hipótesis. Nuestros resultados mostraron que los osos pardos generalmente seleccionaron árboles más conspicuos, con preferencia por los abedules (Betula spp.). Esta elección puede facilitar el marcaje y/o detección de señales químicas y, por tanto, la eficacia de la comunicación intraespecífica. Por el contrario, la abundancia de marcaje a lo largo de los trayectos no parece depender de la densidad de las observaciones de osos o de su posición relativa con respecto al centro o los límites del rango de la población. Nuestros resultados sugieren que los osos pardos cantábricos seleccionan árboles en función de sus características individuales y de su ubicación, sin que influyan en ello las características de la población osera. Nuestros hallazgos pueden servir para localizar árboles específicos que podrían ayudar al monitoreo de la población.

Visual marking in mammals first proved by manipulations of brown bear tree debarking.

The rather limited human ability to understand animal vision and visual signalling has frequently clouded our expectations concerning the visual abilities of other animals. But there are multiple reasons to suspect that visual signalling is more widely employed by animals than previously thought. Because visibility of visual marks depends on the background in which they are seen, species spending most of their time living in dark conditions (e.g., in forests and/or having crepuscular and nocturnal habits) may rely on bright signals to enhance visual display. Here, as a result of experimental manipulations, we present, for the first time ever, evidence supporting the use of a new channel of intraspecific communication by a mammal species, i.e., brown bear Ursus arctos adult males relying on visual marks during mating. Bear reactions to our manipulation suggest that visual signalling could represent a widely overlooked mechanism in mammal communication, which may be more broadly employed than was previously thought.

Denning in brown bears.

Hibernation represents an adaptation for coping with unfavorable environmental conditions. For brown bears Ursus arctos, hibernation is a critical period as pronounced temporal reductions in several physiological functions occur.Here, we review the three main aspects of brown bear denning: (1) den chronology, (2) den characteristics, and (3) hibernation physiology in order to identify (a) proximate and ultimate factors of hibernation as well as (b) research gaps and conservation priorities.Den chronology, which varies by sex and reproductive status, depends on environmental factors, such as snow, temperature, food availability, and den altitude. Significant variation in hibernation across latitudes occurs for both den entry and exit.The choice of a den and its surroundings may affect individual fitness, for example, loss of offspring and excessive energy consumption. Den selection is the result of broad- and fine-scale habitat selection, mainly linked to den insulation, remoteness, and availability of food in the surroundings of the den location.Hibernation is a metabolic challenge for the brown bears, in which a series of physiological adaptations in tissues and organs enable survival under nutritional deprivation, maintain high levels of lipids, preserve muscle, and bone and prevent cardiovascular pathologies such as atherosclerosis. It is important to understand: (a) proximate and ultimate factors in denning behavior and the difference between actual drivers of hibernation (i.e., factors to which bears directly respond) and their correlates; (b) how changes in climatic factors might affect the ability of bears to face global climate change and the human-mediated changes in food availability; (c) hyperphagia (period in which brown bears accumulate fat reserves), predenning and denning periods, including for those populations in which bears do not hibernate every year; and (d) how to approach the study of bear denning merging insights from different perspectives, that is, physiology, ecology, and behavior.

Methodological Considerations for Using Fecal Glucocorticoid Metabolite Concentrations as an Indicator of Physiological Stress in the Brown Bear (Ursus arctos).

Reliable methods to measure stress-related glucocorticoid responses in free-ranging animals are important for wildlife management and conservation. Such methods are also paramount for our ability to improve our knowledge of the ecological consequences of physiological processes. The brown bear (Ursus arctos) is a large carnivore of ecological and cultural importance and is important for management. Here, we provide a physiological validation for an enzyme immunoassay (EIA) to quantify glucocorticoid metabolites in brown bear feces. We also provide an evaluation of the effects of sample exposure to ambient temperature on measured fecal glucocorticoid metabolite (fGCM) concentrations. We evaluated three EIA systems: a cortisol assay, an 11-oxoetiocholanolone assay, and an 11ß-hydroxyetiocholanolone assay. Of these, the cortisol assay provided the best discrimination between peak fGCM concentrations detected 1-4 d after injections of synthetic adrenocorticotrophic hormone and preinjection baseline concentrations in four individual brown bears. The time of exposure to ambient temperature had substantial but variable effects on measured fGCM concentrations, including variation both between samples from the same individual and among samples from different bears. We propose that the validated EIA system for measuring fGCM concentrations in the brown bear could be a useful noninvasive method to monitor stress in this species. However, we highlight that this method requires that fecal samples be frozen immediately after defecation, which could be a limitation in many field situations.

Identifying potential areas of expansion for the endangered brown bear (Ursus arctos) population in the Cantabrian Mountains (NW Spain).

Many large carnivore populations are expanding into human-modified landscapes and the subsequent increase in coexistence between humans and large carnivores may intensify various types of conflicts. A proactive management approach is critical to successful mitigation of such conflicts. The Cantabrian Mountains in Northern Spain are home to the last remaining native brown bear (Ursus arctos) population of the Iberian Peninsula, which is also amongst the most severely threatened European populations, with an important core group residing in the province of Asturias. There are indications that this small population is demographically expanding its range. The identification of the potential areas of brown bear range expansion is crucial to facilitate proactive conservation and management strategies towards promoting a further recovery of this small and isolated population. Here, we used a presence-only based maximum entropy (MaxEnt) approach to model habitat suitability and identify the areas in the Asturian portion of the Cantabrian Mountains that are likely to be occupied in the future by this endangered brown bear population following its range expansion. We used different spatial scales to identify brown bear range suitability according to different environmental, topographic, climatic and human impact variables. Our models mainly show that: (1) 4977 km2 are still available as suitable areas for bear range expansion, which represents nearly half of the territory of Asturias; (2) most of the suitable areas in the western part of the province are already occupied (77% of identified areas, 2820 km2), 41.4% of them occurring inside protected areas, which leaves relatively limited good areas for further expansion in this part of the province, although there might be more suitable areas in surrounding provinces; and (3) in the eastern sector of the Asturian Cantabrian Mountains, 62% (2155 km2) of the land was classified as suitable, and this part of the province hosts 44.3% of the total area identified as suitable areas for range expansion. Our results further highlight the importance of increasing: (a) the connectivity between the currently occupied western part of Asturias and the areas of potential range expansion in the eastern parts of the province; and (b) the protection of the eastern sector of the Cantabrian Mountains, where most of the future population expansion may be expected.

Responses of an endangered brown bear population to climate change based on predictable food resource and shelter alterations.

The survival of an increasing number of species is threatened by climate change: 20%-30% of plants and animals seem to be at risk of range shift or extinction if global warming reaches levels projected to occur by the end of this century. Plant range shifts may determine whether animal species that rely on plant availability for food and shelter will be affected by new patterns of plant occupancy and availability. Brown bears in temperate forested habitats mostly forage on plants and it may be expected that climate change will affect the viability of the endangered populations of southern Europe. Here, we assess the potential impact of climate change on seven plants that represent the main food resources and shelter for the endangered population of brown bears in the Cantabrian Mountains (Spain). Our simulations suggest that the geographic range of these plants might be altered under future climate warming, with most bear resources reducing their range. As a consequence, this brown bear population is expected to decline drastically in the next 50 years. Range shifts of brown bear are also expected to displace individuals from mountainous areas towards more humanized ones, where we can expect an increase in conflicts and bear mortality rates. Additional negative effects might include: (a) a tendency to a more carnivorous diet, which would increase conflicts with cattle farmers; (b) limited fat storage before hibernation due to the reduction of oak forests; (c) increased intraspecific competition with other acorn consumers, that is, wild ungulates and free-ranging livestock; and (d) larger displacements between seasons to find main trophic resources. The magnitude of the changes projected by our models emphasizes that conservation practices focused only on bears may not be appropriate and thus we need more dynamic conservation planning aimed at reducing the impact of climate change in forested landscapes.

Patterns of wild carnivore attacks on humans in urban areas.

Attacks by wild carnivores on humans represent an increasing problem in urban areas across North America and their frequency is expected to rise following urban expansion towards carnivore habitats. Here, we analyzed records of carnivore attacks on humans in urban areas of the U.S. and Canada between 1980 and 2016 to analyze the general patterns of the attacks, as well as describe the landscape structure and, for those attacks occurring at night, the light conditions at the site of the attacks. We found that several behavioral and landscape-related factors were recurrent elements in the attacks recorded. The species for which the attack locations were available (coyote and black bear) attacked in areas with different conditions of landscape structure and artificial light. Specifically, black bears attacked more frequently in areas with abundant and aggregated vegetation cover and scarce buildings and roads, while coyotes attacked in a broader range of landscape conditions. At night, black bears attacked in generally darker areas than coyotes. By providing a comprehensive perspective of the phenomenon, this study will improve our understanding of how effective strategies aimed at reducing the frequency of risky encounters in urban areas should be developed.

Patterns of brown bear damages on apiaries and management recommendations in the Cantabrian Mountains, Spain.

Large carnivores are often persecuted due to conflict with human activities, making their conservation in human-modified landscapes very challenging. Conflict-related scenarios are increasing worldwide, due to the expansion of human activities or to the recovery of carnivore populations. In general, brown bears Ursus arctos avoid humans and their settlements, but they may use some areas close to people or human infrastructures. Bear damages in human-modified landscapes may be related to the availability of food resources of human origin, such as beehives. However, the association of damage events with factors that may predispose bears to cause damages has rarely been investigated. We investigated bear damages to apiaries in the Cantabrian Mountains (Spain), an area with relatively high density of bears. We included spatial, temporal and environmental factors and damage prevention measures in our analyses, as factors that may influence the occurrence and intensity of damages. In 2006-2008, we located 61 apiaries, which included 435 beehives damaged in the study area (346 km2). The probability of an apiary being attacked was positively related to both the intensity of the damage suffered the year before and the distance to the nearest damaged apiary, and negatively related to the number of prevention measures employed as well as the intensity of the damage suffered by the nearest damage apiary. The intensity of damage to apiaries was positively related to the size of the apiary and to vegetation cover in the surroundings, and negatively related to the number of human settlements. Minimizing the occurrence of bear damages to apiaries seems feasible by applying and maintaining proper prevention measures, especially before an attack occurs and selecting appropriate locations for beehives (e.g. away from forest areas). This applies to areas currently occupied by bears, and to neighbouring areas where dispersing individuals may expand their range.

Colour polymorphism in owls is linked to light variability.

Owls show an astonishing variation in their degree of colour polymorphism, although the exact mechanisms driving such variation remain controversial. Here we address this fundamental question by considering information on all extant owls and recent advances in comparative methods in the frame of three mutually non-exclusive evolutionary scenarios. In addition, we study for the first time whether the evolution of influential ecological characters facilitated the evolution of colour polymorphism (or vice versa). In agreement with the niche divergence hypothesis, we found that species living under more variable luminal conditions, i.e., species with diurnal and crepuscular habits and those inhabiting in a mixture of open and closed habitats, were more likely to show colour polymorphism. Correlated evolution analyses revealed that a change in the luminal niche might be a fundamental requisite for the evolution of colour polymorphism. Moreover, polymorphism was more frequent among owl species occupying lower trophic levels, which could be explained by a particularly high selection for crypsis on small predator owls. Our results, thus, provide support for the idea that colour polymorphism in owls is an adaptive character likely maintained by the selective advantage of morphs under different environmental conditions via disruptive selection mechanisms.

The importance of individual variation in the dynamics of animal collective movements.

Animal collective movements are a key example of a system that links two clearly defined levels of organization: the individual and the group. Most models investigating collective movements have generated coherent collective behaviours without the inclusion of individual variability. However, new individual-based models, together with emerging empirical information, emphasize that within-group heterogeneity may strongly influence collective movement behaviour. Here we (i) review the empirical evidence for individual variation in animal collective movements, (ii) explore how theoretical investigations have represented individual heterogeneity when modelling collective movements and (iii) present a model to show how within-group heterogeneity influences the collective properties of a group. Our review underscores the need to consider variability at the level of the individual to improve our understanding of how individual decision rules lead to emergent movement patterns, and also to yield better quantitative predictions of collective behaviour.This article is part of the theme issue 'Collective movement ecology'.

Feather content of porphyrins in Eurasian eagle owl (Bubo bubo) fledglings depends on body condition and breeding site quality.

Porphyrins are pigments produced in most animal cells during the synthesis of heme, but their importance for external coloration is unclear. Owls (Order Strigiformes) are among the few animals that accumulate porphyrins in the integument, where it could serve as a means of signaling. Here we hypothesized that the porphyrin content of feathers may depend on body condition and breeding site quality in Eurasian eagle owl (Bubo bubo) fledglings and, thus, constitute amplifiers of the quality of the area where they are born. Using high-performance liquid chromatography, we found 2 porphyrins (protoporphyrin IX and coproporphyrin III) in the body feathers of 19 eagle owl fledglings from 7 breeding territories. Coproporphyrin III, but not protoporphyrin IX feather concentration, was positively associated with the body mass of fledglings and with the quality of the breeding sites where they were reared with respect to food quality and availability. As coproporphyrin III is produced under oxidative stress, we suggest that good breeding sites may lead to fledglings in good condition. This, in turn, may make fledglings induce a certain level of free radical and coproporphyrin III production to signal to conspecifics their site-mediated capacity to cope with oxidative stress. This is the first time that porphyrin content in the integument has been found to be related to individual quality, opening a new scenario for studying evolution of animal coloration.

Living in the dark does not mean a blind life: bird and mammal visual communication in dim light.

For many years, it was believed that bird and mammal communication 'in the dark of the night' relied exclusively on vocal and chemical signalling. However, in recent decades, several case studies have conveyed the idea that the nocturnal world is rich in visual information. Clearly, a visual signal needs a source of light to work, but diurnal light (twilight included, i.e. any light directly dependent on the sun) is not the only source of luminosity on this planet. Actually, moonlight represents a powerful source of illumination that cannot be neglected from the perspective of visual communication. White patches of feathers and fur on a dark background have the potential to be used to communicate with conspecifics and heterospecifics in dim light across different contexts and for a variety of reasons. Here: (i) we review current knowledge on visual signalling in crepuscular and nocturnal birds and mammals; and (ii) we also present some possible cases of birds and mammals that, due to the characteristics of their feather and fur coloration pattern, might use visual signals in dim light. Visual signalling in nocturnal animals is still an emerging field and, to date, it has received less attention than many other means of communication, including visual communication under daylight. For this reason, many questions remain unanswered and, sometimes, even unasked.This article is part of the themed issue 'Vision in dim light'.

Human behaviour can trigger large carnivore attacks in developed countries.

The media and scientific literature are increasingly reporting an escalation of large carnivore attacks on humans in North America and Europe. Although rare compared to human fatalities by other wildlife, the media often overplay large carnivore attacks on humans, causing increased fear and negative attitudes towards coexisting with and conserving these species. Although large carnivore populations are generally increasing in developed countries, increased numbers are not solely responsible for the observed rise in the number of attacks by large carnivores. Here we show that an increasing number of people are involved in outdoor activities and, when doing so, some people engage in risk-enhancing behaviour that can increase the probability of a risky encounter and a potential attack. About half of the well-documented reported attacks have involved risk-enhancing human behaviours, the most common of which is leaving children unattended. Our study provides unique insight into the causes, and as a result the prevention, of large carnivore attacks on people. Prevention and information that can encourage appropriate human behaviour when sharing the landscape with large carnivores are of paramount importance to reduce both potentially fatal human-carnivore encounters and their consequences to large carnivores.

Subtle Gardeners: Inland Predators Enrich Local Topsoils and Enhance Plant Growth.

Inland vertebrate predators could enrich of nutrients the local top soils in the area surrounding their nests and dens by depositing faeces, urine, and prey remains and, thus, alter the dynamics of plant populations. Surprisingly, and in contrast with convincing evidence from coastal habitats, whether and how this phenomenon occurs in inland habitats is largely uncertain even though these habitats represent a major fraction of the earth's surface. We investigated during two consecutive breeding seasons the potential enrichment of the top-soils associated with inland ground-nesting eagle owls Bubo bubo, as well as its possible consequences in the growth of two common annual grasses in southern Spain. Top-soils associated with owl nests differed strongly and significantly from control top-soils in chemical parameters, mainly fertility-related properties. Specifically, levels of available phosphorus, total nitrogen, organic matter, and available potassium were 49.1, 5.6, 3.1, and 2.7 times higher, respectively, in top-soils associated with owl nests as compared to control top-soils. Germination experiments in chambers indicated that nutrient enrichment by nesting owls enhanced seedling growth in both annual grasses (Phalaris canariensis and Avena sativa), with seedling size being 1.4-1.3 times higher in owl nest top-soils than in control top-soils. Our experimental study revealed that pervasive inland, predatory birds can profoundly enrich the topsoil around their nests and, thus, potentially enhance local vegetation growth. Because diverse inland vertebrate predators are widespread in most habitats they have a strong potential to enhance spatial heterogeneity, impinge on plant communities, and exert an overlooked effect on primary productivity worldwide.