RESUMO
It has been shown experimentally in cell suspensions that sonoporation could be used to deliver the anti-cancer drug Adriamycin hydrochloride (ADR) into Jurkat lymphocytes and that it could also be used to deliver goat anti-rabbit IgG and anti-mouse IgD, antibodies into human peripheral blood mononuclear cells (PBMC) and Jurkat lymphocytes. When ADR was delivered, the delivery efficiency reached 4.80+/-2.04% and control baseline (no ultrasound and no ADR) was 0.17+/-0.14%. When anti-rabbit IgD was delivered, the efficiencies were 34.90+/-1.80% (control baseline was 1.33+/-0.31%) and 32.50+/-4.36% (control baseline was 1.66+/-0.06%) respectively for Jurkat cells and PBMC. When goat anti-rabbit IgG was delivered, the efficiencies were 78.60+/-3.60% (control baseline was 1.60+/-0.00%) and 57.50+/-4.23% (control baseline was 11.30+/-0.81%) respectively for Jurkat cells and PBMC.
Assuntos
Antibióticos Antineoplásicos/farmacocinética , Anticorpos/metabolismo , Permeabilidade da Membrana Celular/efeitos da radiação , Sistemas de Liberação de Medicamentos/métodos , Leucócitos Mononucleares/metabolismo , Fonoforese/métodos , Antibióticos Antineoplásicos/administração & dosagem , Anticorpos/administração & dosagem , Células Cultivadas , Humanos , Células Jurkat , Leucócitos Mononucleares/efeitos dos fármacosRESUMO
A method to prepare liposomes is presented. Liposomes made in our laboratory were characterized acoustically and optically. The phase velocity and attenuation of liposomes in suspension (concentration = 10(9)/mL) were measured, ranging from 2 to 14 MHz, using ultrasound spectroscopy. Anti-rabbit IgG conjugated with Alexafluor 647 was delivered into Jurkat cells in suspension, using the liposomes, by 10 % duty cycle ultrasound tonebursts of 2.2 MHz (the in situ spatial peak-pressure amplitude = 80 W/cm2) with an efficiency of 13 %. It has been experimentally shown that liposomes may be an alternative stable agent to Optison for delivering macromolecules into cells.
Assuntos
Sistemas de Liberação de Medicamentos , Lipossomos , Fonoforese/métodos , Linfócitos T/metabolismo , Albuminas , Animais , Calibragem , Células Cultivadas , Fluorocarbonos , Humanos , Imunoglobulina G/administração & dosagem , Células Jurkat , Microbolhas , Coelhos , Análise Espectral/métodosRESUMO
The nonlinear properties of an encapsulated microbubble of a contrast agent were studied theoretically and experimentally. A modified nonlinear differential equation (Herring equation) was used to describe the radial oscillation of the microbubble and solved numerically. It was found that the nonlinear resonance frequency, at which the peak radial oscillation amplitude occurs, was a decreasing function of the acoustic amplitude of a driving ultrasonic pulse. Optical images of the contrast agent microbubbles under various ultrasonic exposure conditions: 1. sham exposure; 2. 2-MHz spatial peak acoustic pressure = 200 kPa, I(SATA) = 260 mW/cm(2), duty cycle = 7.5%, repetition period = 0.0266 ms; 3. 0.5-MHz spatial peak acoustic pressure = 200 kPa, I(SATA) = 130 mW/cm(2), duty cycle = 7.5%, repetition period = 0.1067 ms; have also shown that the lower-frequency ultrasound (US) excitation (0.5 MHz) is more effective in disruption of the microbubbles due to acoustic inertial cavitation than the higher frequency US (2 MHz).