RESUMO
The aim was to analyze the effect of adipose tissue transplantation on growth differentiation factor-9 (GDF-9), insulin growth factor 1 receptor (IGF1R), and leptin receptor (LEPR) protein expression in ovaries of obese anovulatory mice. Leptin-deficient female (ob/ob) and wild-type mice were divided into untreated ob/ob mice and gonadal white adipose tissue transplanted ob/ob mice, with evaluation after 7, 15, and 45 days and compared to control wild-type mice. The corporal weight and glycemia levels increased in the obese group concomitant with polymicrocyst formation and abundant estrone, mimicking anovulatory disease. In the treated group after 45 days, glycemia, weight, ovarian size, and number of follicles were decreased and corpora lutea were decreased. The analysis of GDF-9 revealed that, whereas control ovaries presented follicular localization, the obese ovary lacked this protein. On the other hand, obese ovaries showed elevated expression of IGF1R that was normalized after the transplantation. Finally, LEPR was reduced in obese ovaries, and adipose tissue transplantation was efficient in returning it to normal levels. In conclusion, the adipose tissue transplantation, especially after 45 days, seems to stimulate ovulation, supported by the fact that several proteins involved in ovulation returned to basal levels.
Assuntos
Fator 9 de Diferenciação de Crescimento/metabolismo , Gordura Intra-Abdominal/transplante , Obesidade/complicações , Ovário/metabolismo , Síndrome do Ovário Policístico/terapia , Receptor IGF Tipo 1/metabolismo , Receptores para Leptina/metabolismo , Animais , Anovulação/etiologia , Anovulação/prevenção & controle , Corpo Lúteo/metabolismo , Corpo Lúteo/patologia , Feminino , Fertilidade , Leptina/genética , Camundongos , Camundongos Knockout , Camundongos Obesos , Tamanho do Órgão , Ovário/patologia , Ovário/fisiopatologia , Ovulação , Síndrome do Ovário Policístico/complicações , Síndrome do Ovário Policístico/metabolismo , Síndrome do Ovário Policístico/fisiopatologia , Tela Subcutânea , Transplante HeterotópicoRESUMO
OBJECTIVE: To evaluate the effects of conjugated equine estrogens (CEE) on the pilocarpine-induced epilepsy in rats. STUDY DESIGN: 40 female rats were divided into: GPC (positive control) presented "status epilepticus" (SE) induced by pilocarpine; GOC (ovariectomized control) only castrated; GNC (negative control) received only saline solution; GPE received pilocarpine, presented SE, castrated and received 50microg/kg CEE treatment; GPV received pilocarpine, castrated and received propylene glycol (vehicle). The animals were monitored by a video system. At the end of observation, the brains removed for later histologic analysis using Neo-Timm and Nissl methods. RESULTS: The GPE presented a reduction in number of seizures compared to GPV. The Neo-Timm analysis showed that GPV had greater sprouting of mossy fibers, with a denser band in the area of the dentate gyrus hilum compared to GPE. On Nissl staining, GPE showed evident neuronal loss in the CA3 area. GPV presented loss in CA1 and dentate gyrus. CONCLUSION: Estrogen may have a protecting effect on the central nervous system.