The acute effects of strength, endurance and concurrent exercises on the Akt/mTOR/p70S6K1 and AMPK signaling pathway responses in rat skeletal muscle

The activation of competing intracellular pathways has been proposed to explain the reduced training adaptations after concurrent strength and endurance exercises (CE). The present study investigated the acute effects of CE, strength exercises (SE), and endurance exercises (EE) on phosphorylated/total ratios of selected AMPK and Akt/mTOR/p70S6K1 pathway proteins in rats. Six animals per exercise group were killed immediately (0 h) and 2 h after each exercise mode. In addition, 6 animals in a non-exercised condition (NE) were killed on the same day and under the same conditions. The levels of AMPK, phospho-Thr172AMPK (p-AMPK), Akt, phospho-Ser473Akt (p-Akt), p70S6K1, phospho-Thr389-p70S6K1 (p-p70S6K1), mTOR, phospho-Ser2448mTOR (p-mTOR), and phospho-Thr1462-TSC2 (p-TSC2) expression were evaluated by immunoblotting in total plantaris muscle extracts. The only significant difference detected was an increase (i.e., 87%) in Akt phosphorylated/total ratio in the CE group 2 h after exercise compared to the NE group (P = 0.002). There were no changes in AMPK, TSC2, mTOR, or p70S6K1 ratios when the exercise modes were compared to the NE condition (P ≥ 0.05). In conclusion, our data suggest that low-intensity and low-volume CE might not blunt the training-induced adaptations, since it did not activate competing intracellular pathways in an acute bout of strength and endurance exercises in rat skeletal muscle.

The Orbital Period of the Accreting Pulsar GX 1+4.

We report strong evidence for a approximately 304 day periodicity in the spin history of the accretion-powered pulsar GX 1+4 that is most probably associated with the orbital period of the system. We have used data from the Burst and Transient Source Experiment on the Compton Gamma-Ray Observatory to show a clear periodic modulation of the pulsar frequency from 1991 to date, in excellent agreement with the ephemeris proposed by Cutler, Dennis, & Dolan in 1986. Our results indicate that the orbital period of GX 1+4 is 303.8+/-1.1 days, making it the widest known low-mass X-ray binary system by more than 1 order of magnitude and putting this long-standing question to rest. A likely scenario for this system is an elliptical orbit in which the neutron star decreases its spin-down rate (or even exhibits a momentary spin-up behavior) at periastron passages due to the higher torque exerted by the accretion disk onto the magnetosphere of the neutron star. These results are not inconsistent with either the X-ray pulsed flux light curve measured by BATSE during the same epoch or the X-ray flux history from the All-Sky Monitor on board the Rossi X-Ray Timing Explorer.

Qualidade das aguas de superfície utilizadas no abastecimento da cidade de Natal/RN, Brasil: aplicacao do I.Q.A. nos pontos captacao

La importancia de la preservación de recursos hídricos es muy tenida en cuenta en los nuevos conceptos de desenvolvimiento,considerando los costos socio-económicos en la cantidad y la degradación de la calidad del agua. Para este trabajo los autores realizaron comparaciones durante un año entre: el Lago de Extremoz y el Lago de Jiqui tomando en cuenta el Indice de Cualidades de Aguas (I.Q.A.), después de un análisis global se estableció que las aguas pueden ser clasificadas entre buenas y óptimas para su uso