RESUMO
A new steroidal saponin was isolated from the bulbs of Allium ampeloprasum L. var. porrum. On the basis of chemical evidence, comprehensive spectroscopic analyses, and comparison with known compounds, its structure was established as (3ß,5α,6ß,25R)-3-{(O-ß-D-glucopyranosyl-(1â3)-ß-D-glucopyranosyl-(1â2)-O-[O-ß-D-glucopyranosyl-(1â3)]-O-ß-D-glucopyranosyl-(1â4)-ß-D-galactopyranosyl)oxy}-6-hydroxyspirostan-2-one (1). Results of the present study indicated that 1 exhibited haemolytic activity in the in vitro assays, and immunological adjuvant activity on the cellular immune response against ovalbumin antigen.
Assuntos
Adjuvantes Imunológicos , Eritrócitos/efeitos dos fármacos , Cebolas/química , Saponinas/química , Saponinas/farmacologia , Espirostanos/química , Espirostanos/farmacologia , Adjuvantes Imunológicos/química , Adjuvantes Imunológicos/farmacologia , Animais , Hemólise , Humanos , Espectroscopia de Ressonância Magnética , Masculino , Camundongos , Conformação Molecular , Ovalbumina/imunologia , Saponinas/imunologia , Espirostanos/imunologia , Esteroides/químicaRESUMO
A new steroidal saponin was isolated from the leaves of Agave shrevei. Its structure was established as 3-[O-beta-D-glucopyranosyl-(1-->2)-O-[O-beta-D-glucopyranosyl-(1-->4)-O-[O-beta-D-glucopyranosyl-(1-->6)]-O-beta-D-glucopyranosyl-(1-->4)-beta-D-galactopyranosyl)-oxy]-(3beta,5alpha,25R)-spirostane. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, HMBC, and HMQC) and chemical conversions. The haemolytic activity of the steroidal saponin was evaluated using an in vitro assay.
Assuntos
Agave/química , Saponinas/química , Saponinas/isolamento & purificação , Espirostanos/química , Espirostanos/isolamento & purificação , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Humanos , Estrutura Molecular , Saponinas/farmacologia , Espirostanos/farmacologiaRESUMO
Five structurally related rotenoids were isolated from the roots of Clitoria fairchildiana. The antiinflammatory activity was investigated using a capillary permeability assay. The protective effect was measured as the inhibition in vascular permeability produced by acetic acid in the peritoneal cavity. The results suggest that for maximum activity the maintenance of the B/C ring junction integrity is important. These findings support the traditional use of Clitoria species extracts in the treatment of inflammatory conditions.
