Assuntos
Micronúcleos com Defeito Cromossômico/induzido quimicamente , Mucosa Bucal/efeitos dos fármacos , Nicotina/toxicidade , Adolescente , Adulto , Análise Citogenética , Feminino , Humanos , Masculino , Micronúcleos com Defeito Cromossômico/estatística & dados numéricos , Testes para Micronúcleos , Mucosa Bucal/patologia , Mucosa Bucal/ultraestrutura , Projetos de Pesquisa , FumarRESUMO
OBJECTIVE: Nowadays, much attention has been focused on the search for new non-invasive methodologies able to predict malignant transformation of oral mucosa cells. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers in buccal mucosa cells. STUDY DESIGN: A total of 24 young, healthy smokers and 14 non-smokers were included in this setting. Individuals had epithelial cells from the cheek mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. RESULTS: Smokers presented more (p < 0.05) micronucleated oral mucosa cells than non-smokers. Tobacco smoke was not able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis. CONCLUSION: In summary, these data indicate that the cigarette is able to induce micronuclei in oral mucosa cells, so the micronucleus test is a suitable method for predicting oral cancer risk.
Assuntos
Transformação Celular Neoplásica/patologia , Análise Citogenética , Micronúcleos com Defeito Cromossômico , Testes para Micronúcleos , Mucosa Bucal/patologia , Neoplasias Bucais/patologia , Fumar/patologia , Adulto , Estudos de Casos e Controles , Transformação Celular Neoplásica/genética , Feminino , Humanos , Masculino , Neoplasias Bucais/etiologia , Neoplasias Bucais/genética , Valor Preditivo dos Testes , Medição de Risco , Fatores de Risco , Fumar/genética , Adulto JovemRESUMO
The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 µL from peripheral blood was collected for the single cell gel (comet) assay. The results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. In addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. In summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.
