[Effect of reproduction of the LPP-3 cyanophage on glutamate dehydrogenase and glutamine synthetase activity in the cyanobacterium Plectonema boryanum]. / Vliianie reproduktsii tsianofaga LPP-3 na aktivnost glutamatdegidrogenazy i glutaminsintetazy v kletakh tsianobakterii Plectonema boryanum.

The effect of cyanophage LPP-3 reproduction on glutamate dehydrogenase and glutamine synthetase (GS) in P boryanum cells have been studied. It was determined that the both reactions are intensified by 135% and 220%, accordingly. Isoenzymes of GS were purified from native and infected cell of cyanobacteria. Their physical-and-chemical properties are different. The cyanophage development probably causes specific modification of the cell enzymes.

[Alanine dehydrogenase of the cyanobacterium Plectonema boryanum in the early period of cyanophage LPP-3 development]. / Alanindegidrogenaza tsianobakterii Plectonema boryanum v rannii period razvitiia tsianofaga LPP-3.

It has been studied how reproduction of LPP-3 in Plectonema boryanum cells influences the alanine dehydrogenase activity. It has been found that immediately after the virus adsorption the enzyme activity falls by 50% and the anabolic reaction is blocked. Physicochemical properties of the enzyme vary as well. An infected cell has one isoenzyme-octamer with pl 9.1-9.2, pH-optimum by action 9-10, molecular weight about 27 kDa.

[Alanine dehydrogenase from the cyanobacterium Plectonema boryanum]. / Alanindegidrogenaza tsianobakterii Plectonema boryanum.

Alanine dehydrogenase activity of Plectonema boryanum cells was studied. It was found, that only NAD(H) may be used as the coenzyme. Kinetical parameters for substrates and coenzymes were determined. 2 pH-optima of action have been established for biosynthetic and anabolic reactions. Two isoenzymes of ADG were cleaned using the methods of ion-exchange chromatography and isoelectric focusing. Some physical-chemical properties were studied: pHopt--9-10 and 4.0; temperature optimum -40 degrees C; pI--8.2 and 6.4; molecular weight--50.65 and 52.48 kDa for ADG-1 and ADG-2, respectively. Both enzymes have the subunit structure. Every enzyme is composed by 4 identical polypeptides.

[Aspartate kinase from the cyanobacteriium Plectonema boryanum infected with the cyanophage LPP-3]. / Aspartatkinaza tsianobakterii Plectonema boryanum, infitsirovannoi tsianofagom LPP-3.

The effect of development of cyanophage infection on the activity of aspartate kinase of Plectonema boryanum has been studied. It has been determined that activity of aspartate kinase increased during early period of reproduction of cyanophage. It coincided in time with the increase of the level of amino acids of aspartate family. Isoenzymes of aspartate kinase were isolated from the infected cells purified and studied. Expression of viral genome is accompanied with the appearance of four new isoenzymes determined by virus. The revealed aspartate kinases are not subject of regulation by amino acids (the end-products of biosynthesis) according to the principle of feed-back inhibition.

[Isolation, purification and various properties of aspartate kinase from the cyanobacterium Plectonema boryanum]. / Vydelenie, ochistka i nekotorye svoistva aspartatkinazy tsianobakterii Plectonema boryanum.

The enzymic activity of aspartate kinase from filamentous cyanobacteria Plectonema boryanum at the logarithmic phase of growth has been studied. Aspartate kinase was purified eleven times and extracted in functionally homogeneous state from the cell-free extract by the methods of ammonium sulphate salting-out, ion-exchange chromatography and isoelectric focusing. Some physical and chemical characteristics of the enzyme have been studied: pI-7.2; optimum pH-9.0; optimum temperature-55 degrees C. The dependence of enzymatic reaction on concentration of substrates, co-enzymes, cofactors and protein has been established. Regulation of aspartate kinase activity by amino acids of the aspartate family has been investigated. L-threonine, L-homoserine and L-isoleucine (0.01-0.001 M) have a pronounced inhibitory effect. L-lysine and L-methionine are not immediate inhibitors of aspartate kinase but intensify the inhibitory effect of threonine by the cumulative mechanism.

[The potentials of sympathectomy in treating lesions of the lower extremities in diabetics]. / Vozmozhnosti simpatéktomii v lechenii porazhenii nizhnikh konechnostei u bol'nykh sakharnym diabetom.

Investigations of the system of general and regional hemostasis in patients with diabetes mellitus and the IVth degree of ischemia of lower extremities have established the development of chronic hypercoagulation-thrombotic forms of disseminated intravascular blood coagulability. Prophylactics of thrombotic complications allows to avoid high amputations in 80% of the patients, to make only necrectomies, small amputations and dermoplasty.