[Effect of T-lymphocytes and interferon-gamma on the stages of multicellular tumor spheroids formation in vitro].

In our work we studied the effect of T lymphocytes and interferon-gamma on the vital activity of the breast cancer cells population and formation of multicellular tumor spheroids. We demonstrated the stages of multicellular tumor spheroids formation and the possible ways to implement antitumor influence of T lymphocytes and interferon-gamma through a change in estrogen receptor and the epidermal growth factor receptor expression. Our results shown a correlation between the type of humoral influance, the degree of expression of estrogen receptor and epidermal growth factor receptor and the intensity of tumor microaggregates formation.

[Cytoprotective effect of neuropeptides on immunocompetent cells (in vitro study)].

Effects of the nootropic neuropeptide drugs cerebrolysin (Ebewe, Austria) and cortexin (Geterofarm, Russia) on the immunocompetent cells (T-lymphocytes of the MT-4 cell line and B-lymphocytes of the Raji cell line) were studied in vitro. The cell viability was evaluated using the MTT test by counting living and dead cells upon incubation under various conditions with a vital stain (Trypan Blue). It is established that cerebrolysin exhibits cytoprotective properties with respect to both T- and B-lymphocytes and favors the survival of immunocompetent cells. In addition, cerebrolysin in certain concentrations produces a proliferative effect on B-lymphocytes, thus stimulating the formation of immune memory B cells. In contrast, cortexin in certain concentrations produces a cytotoxic and antiproliferative action on T-lymphocytes. The parameters of influence for both drugs depend on their concentration in the cell incubation medium, the duration of action, and (most significantly) the cell type. These properties may account for one of the possible mechanisms of the immunoprotective action of cerebrolysin in various neurological states accompanied by immune response disorders.

[Kinetics of growth 2-D and 3-D cell models under influence of microenvironment].

A cocultivation system was developed to investigate the interactions between MCF-7 breast cancer epithelial cells and MT-4 (line human T-cell leukemia) by paracrine mechanism (no contact cocultivation). Viability and proliferation were determined in two fractions of MCF-7 cells from spheroid culture and monolayer. It has been shown that the influence of MT-4 reduced the number of cells MCF-7 in fraction of adhesive cells and did not influence on the suspension fraction. Proliferation was reduced in both fractions without MT-4. Cells viability was different in monolayer and spheroids with MT-4 and without MT-4; in monolayer it was lower, in spheroids it was higher. We think that, MT-4 by paracrine signal way stimulated proliferation MCF 7 cells, reduced adhesive to the bottom and led to MTS formation.