[Immunologic similarity of antigens of residual nuclear proteins from various hepatomas]. / Immunologicheskaia obshchnost' antigenov ostatochnogo belka kletochnykh iader razlichnykh gepatom.

Rabbit antiserum against alkali-insoluble nuclear residual protein of transplantable rat hepatoma 27 reacted with residual proteins during immunodiffusion in agarose gel, provided immunoprecipitation of isolated nuclei and indirect immunofluorescence with Zajdela's rat ascites hepatoma and mouse ascites hepatoma 22a, while the reaction with non-liver tumors--rat Jensen, Yoshida and M 1 sarcomas and mouse Ehrlich ascites carcinoma was negative. Mild indirect immunofluorescence with kidney and spleen sections was eliminated if antiserum was preincubated with acetone powders of these tissues.

[Protein composition and electron microscopy of the residual protein fractions of cell nuclei of certain normal organs and tumors of rats]. / Belkovyi sostav i élektronnaia mikroskopiia fraktsii ostatochnogo belka kletochnykh iader nekotorykh normal'nykh organov i opukholei krys.

A marked difference in polypeptide pattern and electron micrographs of the nuclear residual protein fraction between normal tissues /liver, kidney, spleen/ and tumors /hepatoma 27, Jensen and Yoshida sarcomas/ is revealed. While in normal tissues the polypeptides with molecular weight lower than 26 KD constituted about one half of the total residual protein, in tumors the polypeptides with molecular weight exceeding 100 KD were markedly prevailing. In electron micrographs the uniform filaments with a diameter of 4-5 nm, and periodical structure represented the bulk of the preparation from normal liver, while in the case of hepatoma 27 an amorphous granulated material was predominating.

[Effect of heparin, spermidine and Be2+ ions on the phosphatase and RNAse activity of rat liver cell nuclei]. / Vliianie geparina, spermidina i ionov Be2+ na fosfataznuiu i RNK-aznuiu aktivnost' iader kletok pecheni krys.

Effects of heparin, spermidine, and Be2+ ions on the ATPase and beta-glycerophosphatase and RNA-ase activities of the rat liver cell nuclei were studied. Be2+ was shown to inhibit the ATPase activity and, to a lesser extent, beta-glycerophosphatase activities. Physiological concentrations of heparin and spermidine also lowered the mentioned two activities, as well as the RNAase activity of the nuclei. Evidence is presented for the inhibitory effect of heparin and spermidine on endonucleases.

[Electrophoretic study of the nuclear membrane ribonucleases of rat liver and hepatoma-27 cells]. / Elektroforeticheskoe issledovanie ribonukleaz iadernykh membran kletok pecheni i gepatomy-27 krys.

Purified cell nuclei from the rat liver and hepatoma-27 cells were used to prepare nuclear membranes from which the enzyme-containing extracts of acid-soluble proteins were prepared. The protein extracts were subjected to disc-electrophoresis in 15% polyacrylamide gel using modified Reisfeld's system. It was found that ribonucleases contained in the acid-soluble proteins of the nuclear membranes of normal liver are represented as several components, and differed by their electrophoretic mobility and also by some other physical and chemical properties from crystalline bovine ribonuclease, as well as from nuclear chromatine ribonucleases.

[Phospholipid composition of nuclear membranes and cell nuclei of rat liver and hepatoma 27]. / Fosfolipidnyi sostav iadernykh membran i iader pecheni i gepatomy-27 krys.

Phospholipid composition of nuclei and nuclear membranes from rat liver and hepatoma-27 were investigated. Hepatoma nuclei and nuclear membranes were found to contain cardiolipin which was absent in the same fractions of rat liver. In the nuclei and nuclear membranes of hepatoma the content of sphingomyelin was higher and that of lecithin is lower than in the corresponding subcellular fractions of rat liver. The content of acid phospholipids was much higher in hepatoma nuclear membranes than those of the normal liver. The data obtained show that the general trend of lipid dedifferentiation which earlier was demonstrated for various membranes of different tumor cells is observed also in the case of nuclear membranes.

[Various enzymes of isolated nuclear membranes and cell nuclei of the liver and hepatoma 27 of rats]. / Nekotorye fermenty izolirovannykh iadernykh obolochek i iader kletok pecheni i gepatomy 27 krys.

A comparative study of glucose-6-phosphatase, alcaline RNase, ATPase, inosine diphosphatase and 5'-nucleotidase activities in isolated rat liver and hepatoma-27 nuclei and nuclear envelopes was performed. The tumor nuclear membranes were shown to be free from G-6-Pase activity in contrast to the liver nuclear membranes. The nuclear RNase activity was strongly inhibited in the hepatoma and could be unmasked in the presence of 3-10(-4) M pCMB. Hepatoma nuclear and nuclear envelopes ATP-ase activity was found to be moderately decreased as compared to those of the normal tissue. The values of inosine diphosphatase activity in hepatoma were similar to those in liver. The role of the nuclear envelope in nuclear-cytoplasmic interactions as well as nuclear location of G-6-Pase are discussed.

[Comparative evaluation of chemical methods for determining diethylstilbestrol in tissues of animal origin]. / Sravnitel'naia otsenka khimicheskikh metodov opredeleniia diétilstil'béstrola v tkaniakh zhivotnogo proiskhozhdeniia.

Three chemical methods for determination diethylstilbestrol (DES) in biological material (Jons and Deferage, 1953; Ponder, 1966; Official Method of Pharmacopoeia, USA, 1965) were compared. The most adequate method for determination of DES in animal tissue is the Official method of Pharmacopoeia based on a specific reaction. The method yields reproducible results. Average recovery of pure DES added to biological material was 78%. The method by Jons and Deferage though providing recovery of 86% of the added DES is based on nonspecific reaction with SbCl5, which resulted in too high results. Method of Pharmacopoeia (USA) was used to determine residual amounts of DES in tissues of a rabbit, implanted with 0.1 mg DES per 1 kg weight. One month after implantation, trace amounts of DES were detected only in muscle tissues. Two and three months after implantation, the residual amounts of DES in the rabbit tissues were not detected.