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1.
J Mol Biol ; 435(10): 168069, 2023 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-37003471

RESUMO

The neuronal SNARE protein SNAP25a (isoform 2) forms part of the SNARE complex eliciting synaptic vesicle fusion during neuronal exocytosis. While the post-fusion cis-SNARE complex has been studied extensively, little is known about the pre-fusion conformation of SNAP25a. Here we analyze monomeric SNAP25a by NMR spectroscopy, further supported by small-angle X-ray scattering (SAXS) experiments. SAXS data indicate that monomeric SNAP25 is more compact than a Gaussian chain but still a random coil. NMR shows that for monomeric SNAP25a, before SNAP25a interacts with its SNARE partners to drive membrane fusion, only the N-terminal part (region A5 to V36) of the first SNARE motif, SN1 (L11 - L81), is helical, comprising two α-helices (ranging from A5 to Q20 and S25 toV36). From E37 onwards, SNAP25a is mostly disordered and displays high internal flexibility, including the C-terminal part of SN1, almost the entire second SNARE motif (SN2, N144-A199), and the connecting loop region. Apart from the N-terminal helices, only the C-termini of both SN1 (E73 - K79) and SN2 (region T190 - A199), as well as two short regions in the connecting loop (D99 - K102 and E123 - M127) show a weak α-helical propensity (α-helical population < 25%). We speculate that the N-terminal helices (A5 to Q20 and S25 to V36) which constitute the N-terminus of SN1 act as a nucleation site for initiating SNARE zippering.


Assuntos
Fusão de Membrana , Neurônios , Proteínas SNARE , Neurônios/metabolismo , Conformação Proteica , Espalhamento a Baixo Ângulo , Proteínas SNARE/metabolismo , Difração de Raios X , Humanos
2.
Int J Mol Sci ; 23(16)2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-36012438

RESUMO

The transient outward potassium current (Itof) is generated by the activation of KV4 channels assembled with KChIP2 and other accessory subunits (DPP6 and KCNE2). To test the hypothesis that these subunits modify the channel pharmacology, we analyzed the electrophysiological effects of (3-(2-(3-phenoxyphenyl)acetamido)-2-naphthoic acid) (IQM-266), a new KChIP2 ligand, on the currents generated by KV4.3/KChIP2, KV4.3/KChIP2/DPP6 and KV4.3/KChIP2/KCNE2 channels. CHO cells were transiently transfected with cDNAs codifying for different proteins (KV4.3/KChIP2, KV4.3/KChIP2/DPP6 or KV4.3/KChIP2/KCNE2), and the potassium currents were recorded using the whole-cell patch-clamp technique. IQM-266 decreased the maximum peak of KV4.3/KChIP2, KV4.3/KChIP2/DPP6 and KV4.3/KChIP2/KCNE2 currents, slowing their time course of inactivation in a concentration-, voltage-, time- and use-dependent manner. IQM-266 produced an increase in the charge in KV4.3/KChIP2 channels that was intensified when DPP6 was present and abolished in the presence of KCNE2. IQM-266 induced an activation unblocking effect during the application of trains of pulses to cells expressing KV4.3/KChIP2 and KV4.3/KChIP2/KCNE2, but not in KV4.3/KChIP2/DPP6 channels. Overall, all these results are consistent with a preferential IQM-266 binding to an active closed state of Kv4.3/KChIP2 and Kv4.3/KChIP2/KCNE2 channels, whereas in the presence of DPP6, IQM-266 binds preferentially to an inactivated state. In conclusion, DPP6 and KCNE2 modify the pharmacological response of KV4.3/KChIP2 channels to IQM-266.


Assuntos
Proteínas Interatuantes com Canais de Kv , Canais de Potássio Shal , Animais , Cricetinae , Cricetulus , Proteínas Interatuantes com Canais de Kv/genética , Proteínas Interatuantes com Canais de Kv/metabolismo , Técnicas de Patch-Clamp , Potássio/metabolismo , Canais de Potássio Shal/genética , Canais de Potássio Shal/metabolismo
3.
Nano Lett ; 22(3): 1449-1455, 2022 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-34855407

RESUMO

A mechanism for full-length synaptotagmin-1 (syt-1) to interact with anionic bilayers and to promote fusion in the presence of SNAREs is proposed. Colloidal probe force spectroscopy in conjunction with tethered particle motion monitoring showed that in the absence of Ca2+ the binding of syt-1 to membranes depends on the presence and content of PI(4,5)P2. Addition of Ca2+ switches the interaction forces from weak to strong, eventually exceeding the cohesion of the C2A domain of syt-1 leading to partial unfolding of the protein. Fusion of single unilamellar vesicles equipped with syt-1 and synaptobrevin 2 with planar pore-spanning target membranes containing PS and PI(4,5)P2 shows an almost complete suppression of stalled intermediate fusion states and an accelerated fusion kinetics in the presence of Ca2+, which is further enhanced upon addition of ATP.


Assuntos
Cálcio , Fosfatidilinositol 4,5-Difosfato , Proteínas SNARE , Sinaptotagmina I , Cálcio/química , Cálcio/metabolismo , Cinética , Fusão de Membrana , Fosfatidilinositol 4,5-Difosfato/química , Fosfatidilinositol 4,5-Difosfato/metabolismo , Desdobramento de Proteína , Sinaptotagmina I/química , Sinaptotagmina I/metabolismo
4.
ACS Sens ; 6(10): 3632-3639, 2021 10 22.
Artigo em Inglês | MEDLINE | ID: mdl-34498459

RESUMO

Unraveling cellular physiological processes via luminescent probes that target specific cellular microenvironments is quite challenging due to the uneven distribution of probes. Herein, we designed a new dynamic excimer (DYNEX) imaging method that involves the sensitive detection of nanosecond-scale dynamic molecular contacts of a fluorescent acridone derivative and reveals the cell microenvironment polarity. Using our method, we specifically tracked cell lipid droplets in fibroblast colon carcinoma cells. These organelles play a central role in metabolic pathways, acting as energy reservoirs in regulatory processes. DYNEX imaging provides the inner polarity of cell lipid droplets, which can be related to lipid contents and metabolic dysfunctions. This new methodology will inspire development of novel multidimensional fluorescent sensors that are able to provide target-specific and orthogonal information at the nanosecond scale.


Assuntos
Corantes Fluorescentes , Gotículas Lipídicas , Microscopia de Fluorescência , Imagem Óptica
5.
Arch Biochem Biophys ; 709: 108966, 2021 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-34139199

RESUMO

Chemical neurotransmission is the major mechanism of neuronal communication. Neurotransmitters are released from secretory organelles, the synaptic vesicles (SVs) via exocytosis into the synaptic cleft. Fusion of SVs with the presynaptic plasma membrane is balanced by endocytosis, thus maintaining the presynaptic membrane at steady-state levels. The protein machineries responsible for exo- and endocytosis have been extensively investigated. In contrast, less is known about the role of lipids in synaptic transmission and how the lipid composition of SVs is affected by dynamic exo-endocytotic cycling. Here we summarize the current knowledge about the composition, organization, and function of SV membrane lipids. We also cover lipid biogenesis and maintenance during the synaptic vesicle cycle.


Assuntos
Membranas Sinápticas/química , Vesículas Sinápticas/química , Animais , Endocitose/fisiologia , Exocitose/fisiologia , Humanos , Lipídeos de Membrana/química , Lipídeos de Membrana/metabolismo , Membranas Sinápticas/metabolismo , Vesículas Sinápticas/metabolismo
6.
Nat Commun ; 12(1): 858, 2021 02 08.
Artigo em Inglês | MEDLINE | ID: mdl-33558502

RESUMO

Synaptic vesicles are storage organelles for neurotransmitters. They pass through a trafficking cycle and fuse with the pre-synaptic membrane when an action potential arrives at the nerve terminal. While molecular components and biophysical parameters of synaptic vesicles have been determined, our knowledge on the protein interactions in their membranes is limited. Here, we apply cross-linking mass spectrometry to study interactions of synaptic vesicle proteins in an unbiased approach without the need for specific antibodies or detergent-solubilisation. Our large-scale analysis delivers a protein network of vesicle sub-populations and functional assemblies including an active and an inactive conformation of the vesicular ATPase complex as well as non-conventional arrangements of the luminal loops of SV2A, Synaptophysin and structurally related proteins. Based on this network, we specifically target Synaptobrevin-2, which connects with many proteins, in different approaches. Our results allow distinction of interactions caused by 'crowding' in the vesicle membrane from stable interaction modules.


Assuntos
Reagentes de Ligações Cruzadas/química , Espectrometria de Massas , Membranas Sinápticas/metabolismo , Vesículas Sinápticas/metabolismo , Animais , Encéfalo/metabolismo , Fusão de Membrana , Ligação Proteica , Mapas de Interação de Proteínas , Proteolipídeos , Proteoma/metabolismo , Ratos , Membranas Sinápticas/ultraestrutura , Vesículas Sinápticas/ultraestrutura , Sinaptofisina/metabolismo , ATPases Vacuolares Próton-Translocadoras/metabolismo , Proteína 2 Associada à Membrana da Vesícula/metabolismo
7.
Methods Mol Biol ; 2251: 205-214, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33481242

RESUMO

PROPPINs (ß-propellers that bind polyphosphoinositides) are a protein family that binds preferentially phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) via its FRRG motif. PROPPINs are involved in autophagic functions, but their molecular mechanism is still elusive. To unravel the molecular mechanism of PROPPINs, it is essential to understand the PROPPIN-phosphoinositide binding. Here, we describe a protocol to study the kinetics of the PROPPIN-phosphoinositide binding using a fluorescence resonance energy transfer (FRET) stopped-flow approach. We use FRET between fluorophore-labeled protein and fluorophore-labeled liposomes, monitoring the increase of the acceptor emission in labeled liposomes after the protein-membrane binding. Through this approach, we studied the kinetics of the PROPPIN Atg18 (Autophagy-related protein 18) from Pichia angusta (PaAtg18) and a mutant of its FRRG motif, called FTTG mutant. Stopped-flow experiments demonstrated that the main function of the FRRG motif is to retain, instead of to drive, Atg18 to the membrane, decreasing the Atg18 dissociation rate. Furthermore, this method is suitable for the study of other PI-binding proteins.


Assuntos
Proteínas Relacionadas à Autofagia/farmacocinética , Transferência Ressonante de Energia de Fluorescência/métodos , Proteínas de Membrana/farmacocinética , Ligação Proteica/fisiologia , Proteínas de Saccharomyces cerevisiae/farmacocinética , Autofagia , Proteínas Relacionadas à Autofagia/metabolismo , Proteínas de Transporte/metabolismo , Proteínas de Membrana/metabolismo , Fosfatos de Fosfatidilinositol/metabolismo , Fosfatidilinositóis/metabolismo , Conformação Proteica em Folha beta/fisiologia , Domínios Proteicos/fisiologia , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomycetales/metabolismo , Espectrometria de Fluorescência/métodos , Vacúolos/metabolismo
8.
Proc Natl Acad Sci U S A ; 116(18): 8699-8708, 2019 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-30975750

RESUMO

Intrinsically disordered proteins (IDPs) and their conformational transitions play an important role in neurotransmitter release at the neuronal synapse. Here, the SNARE proteins are essential by forming the SNARE complex that drives vesicular membrane fusion. While it is widely accepted that the SNARE proteins are intrinsically disordered in their monomeric prefusion form, important mechanistic aspects of this prefusion conformation and its lipid interactions, before forming the SNARE complex, are not fully understood at the molecular level and remain controversial. Here, by a combination of NMR and fluorescence spectroscopy methods, we find that vesicular synaptobrevin-2 (syb-2) in its monomeric prefusion conformation shows high flexibility, characteristic for an IDP, but also a high dynamic range and increasing rigidity from the N to C terminus. The gradual increase in rigidity correlates with an increase in lipid binding affinity from the N to C terminus. It could also explain the increased rate for C-terminal SNARE zippering, known to be faster than N-terminal SNARE zippering. Also, the syb-2 SNARE motif and, in particular, the linker domain show transient and weak membrane binding, characterized by a high off-rate and low (millimolar) affinity. The transient membrane binding of syb-2 may compensate for the repulsive forces between the two membranes and/or the SNARE motifs and the membranes, helping to destabilize the hydrophilic-hydrophobic boundary in the bilayer. Therefore, we propose that optimum flexibility and membrane binding of syb-2 regulate SNARE assembly and minimize repulsive forces during membrane fusion.


Assuntos
Lipídeos/química , Proteínas SNARE/metabolismo , Proteína 2 Associada à Membrana da Vesícula/metabolismo , Animais , Espectroscopia de Ressonância Magnética , Neurônios/metabolismo , Ligação Proteica , Proteínas R-SNARE/química , Proteínas R-SNARE/metabolismo , Proteínas SNARE/química , Proteína 2 Associada à Membrana da Vesícula/química
9.
Nat Commun ; 9(1): 4532, 2018 10 31.
Artigo em Inglês | MEDLINE | ID: mdl-30382094

RESUMO

The molecular chaperone Hsp90 is critical for the maintenance of cellular homeostasis and represents a promising drug target. Despite increasing knowledge on the structure of Hsp90, the molecular basis of substrate recognition and pro-folding by Hsp90/co-chaperone complexes remains unknown. Here, we report the solution structures of human full-length Hsp90 in complex with the PPIase FKBP51, as well as the 280 kDa Hsp90/FKBP51 complex bound to the Alzheimer's disease-related protein Tau. We reveal that the FKBP51/Hsp90 complex, which synergizes to promote toxic Tau oligomers in vivo, is highly dynamic and stabilizes the extended conformation of the Hsp90 dimer resulting in decreased Hsp90 ATPase activity. Within the ternary Hsp90/FKBP51/Tau complex, Hsp90 serves as a scaffold that traps the PPIase and nucleates multiple conformations of Tau's proline-rich region next to the PPIase catalytic pocket in a phosphorylation-dependent manner. Our study defines a conceptual model for dynamic Hsp90/co-chaperone/client recognition.


Assuntos
Proteínas de Choque Térmico HSP90/química , Proteínas de Choque Térmico HSP90/toxicidade , Proteínas de Ligação a Tacrolimo/química , Proteínas de Ligação a Tacrolimo/toxicidade , Proteínas tau/química , Proteínas tau/toxicidade , Biocatálise/efeitos dos fármacos , Proteínas de Choque Térmico HSP90/metabolismo , Humanos , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Fosforilação/efeitos dos fármacos , Ligação Proteica/efeitos dos fármacos , Conformação Proteica , Proteínas de Ligação a Tacrolimo/metabolismo , Proteínas tau/metabolismo
10.
Nat Commun ; 8(1): 678, 2017 10 30.
Artigo em Inglês | MEDLINE | ID: mdl-29084947

RESUMO

Autophagy-mediated degradation of synaptic components maintains synaptic homeostasis but also constitutes a mechanism of neurodegeneration. It is unclear how autophagy of synaptic vesicles and components of presynaptic active zones is regulated. Here, we show that Pleckstrin homology containing family member 5 (Plekhg5) modulates autophagy of synaptic vesicles in axon terminals of motoneurons via its function as a guanine exchange factor for Rab26, a small GTPase that specifically directs synaptic vesicles to preautophagosomal structures. Plekhg5 gene inactivation in mice results in a late-onset motoneuron disease, characterized by degeneration of axon terminals. Plekhg5-depleted cultured motoneurons show defective axon growth and impaired autophagy of synaptic vesicles, which can be rescued by constitutively active Rab26. These findings define a mechanism for regulating autophagy in neurons that specifically targets synaptic vesicles. Disruption of this mechanism may contribute to the pathophysiology of several forms of motoneuron disease.


Assuntos
Autofagia/genética , Fatores de Troca do Nucleotídeo Guanina/genética , Doença dos Neurônios Motores/genética , Vesículas Sinápticas/metabolismo , Animais , Linhagem Celular , Células Cultivadas , Fatores de Troca do Nucleotídeo Guanina/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Microscopia Eletrônica de Transmissão , Doença dos Neurônios Motores/metabolismo , Neurônios Motores/metabolismo , Terminações Pré-Sinápticas/metabolismo , Vesículas Sinápticas/ultraestrutura , Proteínas rab de Ligação ao GTP/genética , Proteínas rab de Ligação ao GTP/metabolismo
11.
Sci Rep ; 7(1): 14008, 2017 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-29070817

RESUMO

PROPPINs (ß-propellers that bind polyphosphoinositides) are PtdIns3P and PtdIns(3,5)P2 binding autophagy related proteins. They contain two phosphatidylinositolphosphate (PIP) binding sites and a conserved FRRG motif is essential for PIP binding. Here we present the 2.0 Å resolution crystal structure of the PROPPIN Atg18 from Pichia angusta. We designed cysteine mutants for labelling with the fluorescence dyes to probe the distances of the mutants to the membrane. These measurements support a model for PROPPIN-membrane binding, where the PROPPIN sits in a perpendicular or slightly tilted orientation on the membrane. Stopped-flow measurements suggest that initial PROPPIN-membrane binding is driven by non-specific PIP interactions. The FRRG motif then retains the protein in the membrane by binding two PIP molecules as evident by a lower dissociation rate for Atg18 in comparison with its PIP binding deficient FTTG mutant. We demonstrate that the amine-specific cross-linker Bis(sulfosuccinimidyl)suberate (BS3), which is used for protein-protein cross-linking can also be applied for cross-linking proteins and phosphatidylethanolamine (PE). Cross-linking experiments with liposome bound Atg18 yielded several PE cross-linked peptides. We also observed intermolecular cross-linked peptides, which indicated Atg18 oligomerization. FRET-based stopped-flow measurements revealed that Atg18 rapidly oligomerizes upon membrane binding while it is mainly monomeric in solution.


Assuntos
Proteínas Relacionadas à Autofagia/química , Proteínas Relacionadas à Autofagia/metabolismo , Autofagia , Membrana Celular/metabolismo , Fosfatidilinositóis/metabolismo , Pichia/metabolismo , Multimerização Proteica , Sítios de Ligação , Membrana Celular/química , Cristalização , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Fosfatidilinositóis/química , Ligação Proteica , Conformação Proteica
12.
Elife ; 52016 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-27791979

RESUMO

The Ca2+-sensor synaptotagmin-1 that triggers neuronal exocytosis binds to negatively charged membrane lipids (mainly phosphatidylserine (PtdSer) and phosphoinositides (PtdIns)) but the molecular details of this process are not fully understood. Using quantitative thermodynamic, kinetic and structural methods, we show that synaptotagmin-1 (from Rattus norvegicus and expressed in Escherichia coli) binds to PtdIns(4,5)P2 via a polybasic lysine patch in the C2B domain, which may promote the priming or docking of synaptic vesicles. Ca2+ neutralizes the negative charges of the Ca2+-binding sites, resulting in the penetration of synaptotagmin-1 into the membrane, via binding of PtdSer, and an increase in the affinity of the polybasic lysine patch to phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2). These Ca2+-induced events decrease the dissociation rate of synaptotagmin-1 membrane binding while the association rate remains unchanged. We conclude that both membrane penetration and the increased residence time of synaptotagmin-1 at the plasma membrane are crucial for triggering exocytotic membrane fusion.


Assuntos
Cálcio/metabolismo , Membrana Celular/metabolismo , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosfatidilserinas/metabolismo , Sinaptotagmina I/metabolismo , Animais , Escherichia coli/genética , Expressão Gênica , Ratos , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Sinaptotagmina I/genética
13.
Nat Struct Mol Biol ; 22(10): 815-23, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26389740

RESUMO

The Ca(2+) sensor synaptotagmin-1 is thought to trigger membrane fusion by binding to acidic membrane lipids and SNARE proteins. Previous work has shown that binding is mediated by electrostatic interactions that are sensitive to the ionic environment. However, the influence of divalent or polyvalent ions, at physiological concentrations, on synaptotagmin's binding to membranes or SNAREs has not been explored. Here we show that binding of rat synaptotagmin-1 to membranes containing phosphatidylinositol 4,5-bisphosphate (PIP2) is regulated by charge shielding caused by the presence of divalent cations. Surprisingly, polyvalent ions such as ATP and Mg(2+) completely abrogate synaptotagmin-1 binding to SNAREs regardless of the presence of Ca(2+). Altogether, our data indicate that at physiological ion concentrations Ca(2+)-dependent synaptotagmin-1 binding is confined to PIP2-containing membrane patches in the plasma membrane, suggesting that membrane interaction of synaptotagmin-1 rather than SNARE binding triggers exocytosis of vesicles.


Assuntos
Membrana Celular/metabolismo , Exocitose/fisiologia , Modelos Moleculares , Fosfatidilinositol 4,5-Difosfato/metabolismo , Sinaptotagmina I/química , Sinaptotagmina I/metabolismo , Animais , Cálcio/metabolismo , Cátions Bivalentes/metabolismo , Grânulos Cromafim/metabolismo , Cromatografia por Troca Iônica , Polarização de Fluorescência , Transferência Ressonante de Energia de Fluorescência , Modelos Teóricos , Técnicas de Patch-Clamp , Conformação Proteica , Ratos , Proteínas SNARE/metabolismo , Análise Espectral
14.
Biophys J ; 108(9): 2223-34, 2015 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-25954880

RESUMO

PROPPINs (ß-propellers that bind polyphosphoinositides) are a family of PtdIns3P- and PtdIns(3,5)P2-binding proteins that play an important role in autophagy. We analyzed PROPPIN-membrane binding through isothermal titration calorimetry (ITC), stopped-flow measurements, mutagenesis studies, and molecular dynamics (MD) simulations. ITC measurements showed that the yeast PROPPIN family members Atg18, Atg21, and Hsv2 bind PtdIns3P and PtdIns(3,5)P2 with high affinities in the nanomolar to low-micromolar range and have two phosphoinositide (PIP)-binding sites. Single PIP-binding site mutants have a 15- to 30-fold reduced affinity, which explains the requirement of two PIP-binding sites in PROPPINs. Hsv2 bound small unilamellar vesicles with a higher affinity than it bound large unilamellar vesicles in stopped-flow measurements. Thus, we conclude that PROPPIN membrane binding is curvature dependent. MD simulations revealed that loop 6CD is an anchor for membrane binding, as it is the region of the protein that inserts most deeply into the lipid bilayer. Mutagenesis studies showed that both hydrophobic and electrostatic interactions are required for membrane insertion of loop 6CD. We propose a model for PROPPIN-membrane binding in which PROPPINs are initially targeted to membranes through nonspecific electrostatic interactions and are then retained at the membrane through PIP binding.


Assuntos
Proteínas de Transporte/química , Fosfatidilinositóis/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Sequência de Aminoácidos , Proteínas de Transporte/metabolismo , Endopeptidases/química , Endopeptidases/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Dados de Sequência Molecular , Fosfatidilinositóis/química , Ligação Proteica , Estrutura Terciária de Proteína , Proteínas de Saccharomyces cerevisiae/metabolismo , Eletricidade Estática , Lipossomas Unilamelares/química , Lipossomas Unilamelares/metabolismo , Leveduras/metabolismo
16.
Nat Commun ; 5: 5859, 2014 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-25500905

RESUMO

Fast synchronous neurotransmitter release is triggered by calcium that activates synaptotagmin-1 (syt-1), resulting in fusion of synaptic vesicles with the presynaptic membrane. Syt-1 possesses two Ca(2+)-binding C2 domains that tether membranes via interactions with anionic phospholipids. It is capable of crosslinking membranes and has recently been speculated to trigger fusion by decreasing the gap between them. As quantitative information on membrane gaps is key to understanding general cellular mechanisms, including the role of syt-1, we developed a fluorescence-lifetime based inter-membrane distance ruler using membrane-anchored DNAs of various lengths as calibration standards. Wild-type and mutant data provide evidence that full-length syt-1 indeed regulates membrane gaps: without Ca(2+), syt-1 maintains membranes at distances of ~7-8 nm. Activation with 100 µM Ca(2+) decreases the distance to ~5 nm by binding the C2 domains to opposing membranes, respectively. These values reveal that activated syt-1 adjusts membrane distances to the level that promotes SNARE complex assembly.


Assuntos
Oligonucleotídeos/química , Terminações Pré-Sinápticas/química , Proteínas SNARE/química , Vesículas Sinápticas/química , Sinaptotagmina I/química , Animais , Cálcio/química , Ácidos Carboxílicos , Cátions Bivalentes , Colesterol/química , Corantes Fluorescentes , Expressão Gênica , Microscopia de Fluorescência , Oligonucleotídeos/síntese química , Fosfatidiletanolaminas/química , Fosfatidilserinas/química , Terminações Pré-Sinápticas/metabolismo , Proteolipídeos/química , Ratos , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Proteínas SNARE/metabolismo , Vesículas Sinápticas/metabolismo , Sinaptotagmina I/metabolismo , Lipossomas Unilamelares/química
17.
PLoS One ; 9(4): e95973, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24763383

RESUMO

The C2 domain of PKCα (C2α) induces fluorescence self-quenching of NBD-PS in the presence of Ca2+, which is interpreted as the demixing of phosphatidylserine from a mixture of this phospholipid with phosphatidylcholine. Self-quenching of NBD-PS was considerably increased when phosphatidylinositol-4,5-bisphosphate (PIP2) was present in the membrane. When PIP2 was the labeled phospholipid, in the form of TopFluor-PIP2, fluorescence self-quenching induced by the C2 domain was also observed, but this was dependent on the presence of phosphatidylserine. An independent indication of the phospholipid demixing effect given by the C2α domain was obtained by using 2H-NMR, since a shift of the transition temperature of deuterated phosphatidylcholine was observed as a consequence of the addition of the C2α domain, but only in the presence of PIP2. The demixing induced by the C2α domain may have a physiological significance since it means that the binding of PKCα to membranes is accompanied by the formation of domains enriched in activating lipids, like phosphatidylserine and PIP2. The formation of these domains may enhance the activation of the enzyme when it binds to membranes containing phosphatidylserine and PIP2.


Assuntos
Fosfatidilcolinas/química , Fosfatidiletanolaminas/química , Fosfatidilinositol 4,5-Difosfato/química , Proteína Quinase C-alfa/química , Cálcio/química , Cátions Bivalentes , Fluorescência , Membranas Artificiais , Estrutura Terciária de Proteína
18.
PLoS One ; 8(7): e69041, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23874859

RESUMO

The C2 domain of PKCα possesses two different binding sites, one for Ca(2+) and phosphatidylserine and a second one that binds PIP2 with very high affinity. The enzymatic activity of PKCα was studied by activating it with large unilamellar lipid vesicles, varying the concentration of Ca(2+) and the contents of dioleylglycerol (DOG), phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphadidylserine (POPS) in these model membranes. The results showed that PIP2 increased the Vmax of PKCα and, when the PIP2 concentration was 5 mol% of the total lipid in the membrane, the addition of 2 mol% of DOG did not increase the activity. In addition PIP2 decreases K0.5 of Ca(2+) more than 3-fold, that of DOG almost 5-fold and that of POPS by a half. The K0.5 values of PIP2 amounted to only 0.11 µM in the presence of DOG and 0.39 in its absence, which is within the expected physiological range for the inner monolayer of a mammalian plasma membrane. As a consequence, PKCα may be expected to operate near its maximum capacity even in the absence of a cell signal producing diacylglycerol. Nevertheless, we have shown that the presence of DOG may also help, since the K0.5 for PIP2 notably decreases in its presence. Taken together, these results underline the great importance of PIP2 in the activation of PKCα and demonstrate that in its presence, the most important cell signal for triggering the activity of this enzyme is the increase in the concentration of cytoplasmic Ca(2+).


Assuntos
Cálcio/farmacologia , Diglicerídeos/farmacologia , Fosfatidilinositol 4,5-Difosfato/farmacologia , Fosfatidilserinas/farmacologia , Proteína Quinase C-alfa/metabolismo , Animais , Cálcio/análise , Diglicerídeos/análise , Relação Dose-Resposta a Droga , Regulação para Baixo/efeitos dos fármacos , Sinergismo Farmacológico , Ativação Enzimática/efeitos dos fármacos , Modelos Moleculares , Concentração Osmolar , Fosfatidilserinas/análise , Ligação Proteica , Proteína Quinase C-alfa/química , Ratos , Células Sf9 , Spodoptera
19.
Biochim Biophys Acta ; 1821(11): 1434-42, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22842589

RESUMO

Protein kinase Cα (PKCα) is activated by its translocation to the membrane. Activity assays show the importance of PIP(2) in determining the specific activity of this enzyme. A FRET stopped flow fluorescence study was carried out to monitor the rapid kinetics of protein binding to model membranes containing POPC/POPS/DOG and eventually PIP(2). The results best fitted a binding mechanism in which protein bound to the membrane following a two-phase mechanism with a first bimolecular reaction followed by a slow unimolecular reaction. In the absence of PIP(2), the rapid protein binding rate was especially dependent on POPS concentration. Formation of the slow high affinity complex during the second phase seems to involve specific interactions with POPS and DOG since it is only sensitive to changes within relatively low concentration ranges of these lipids. Both the association and dissociation rate constants fell in the presence of PIP(2). We propose a model in which PKCα binds to the membranes via a two-step mechanism consisting of the rapid membrane initial recruitment of PKCα driven by interactions with POPS and/or PIP(2) although interactions with DOG are involved too. PKCα searches on the lipid bilayer in two dimensions to establish interactions with its specific ligands.


Assuntos
Membrana Celular/metabolismo , Diglicerídeos/metabolismo , Lipídeos de Membrana/metabolismo , Fosfatidilcolinas/metabolismo , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosfatidilserinas/metabolismo , Proteína Quinase C-alfa/metabolismo , Animais , Transferência Ressonante de Energia de Fluorescência , Cinética , Bicamadas Lipídicas/metabolismo , Modelos Moleculares , Ligação Proteica , Proteína Quinase C-alfa/isolamento & purificação , Estrutura Terciária de Proteína , Espectrometria de Fluorescência , Suínos
20.
Arch Biochem Biophys ; 513(1): 36-41, 2011 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-21741352

RESUMO

Curcumin modulates the activity of protein kinase Cα (PKCα) when assayed in the presence of vesicles including phosphatidylcholine, phosphatidylserine and diacylglycerol. Increasing concentrations of curcumin progressively increased PKCα activity at concentrations lower than 20µM, but at higher concentrations of curcumin the activity decreased although, at concentrations of curcumin of up to 100µM the activity was always higher than the basal one (in the absence of curcumin). The maximum activity was reached at 3µM curcumin, at 20 and 30mol% of phosphatidylserine, 10µM Ca(2+) and 2mol% diacylglycerol. The same type of modulation was observed when changing the concentration of phosphatidylserine, diacylglycerol and Ca(2+). No effect of curcumin was found when the activity was assayed in the presence of Triton X-100 mixed micelles which included phosphatidylserine and diacylglycerol, indicating that the effect of curcumin was membrane-dependent. The pattern of binding of PKCα to membrane vesicles as a function of curcumin concentration closely correlated with the pattern of activating effect. It was concluded that the effect of curcumin on PKCα activity was related to its effect on the membrane, which may modulate the binding of the enzyme to the membrane.


Assuntos
Curcumina/química , Membranas Artificiais , Proteína Quinase C-alfa/química , Cálcio/química , Cálcio/metabolismo , Curcumina/metabolismo , Humanos , Lipídeos/química , Micelas , Octoxinol/química , Ligação Proteica , Proteína Quinase C-alfa/metabolismo
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