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1.
Phytochemistry ; 68(1): 8-18, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17081575

RESUMO

Legume plants are able to enter two different endosymbioses with soil prokaryotes and soil fungi, leading to nitrogen-fixing root nodules and to arbuscular mycorrhiza (AM), respectively. We applied in silico and microarray-based transcriptome profiling approaches to uncover the transcriptome of developing root nodules and AM roots of the model legume Medicago truncatula. Several hundred genes were found to be activated in different stages of either symbiosis, with almost 100 genes being co-induced during nodulation and in arbuscular mycorrhiza. These co-induced genes can be associated with different cellular functions required for symbiotic efficiency, such as the facilitation of transport processes across the perisymbiotic membranes that surround the endosymbiotic bacteroids in root nodules and the arbuscules in AM roots. To specify promoter elements required for gene expression in arbuscule-containing cells, reporter gene fusions of the promoter of the Vicia faba leghemoglobin gene VfLb29 were studied by loss-of-function and gain-of-function approaches in transgenic hairy roots. These analyses specified a 85-bp fragment that was necessary for gene expression in arbuscule-containing cells but was dispensible for gene activation in root nodules. In contrast to promoters mediating gene expression in the infected cells of root nodules, the activation of genes in AM appears to be governed by more complex regulatory systems requiring different promoter modules.


Assuntos
Fabaceae/genética , Fabaceae/metabolismo , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Simbiose/fisiologia , Nódulos Radiculares de Plantas , Transcrição Gênica , Ativação Transcricional
2.
Phytochemistry ; 68(1): 19-32, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17081576

RESUMO

The great majority of terrestrial plants enters a beneficial arbuscular mycorrhiza (AM) or ectomycorrhiza (ECM) symbiosis with soil fungi. In the SPP 1084 "MolMyk: Molecular Basics of Mycorrhizal Symbioses", high-throughput EST-sequencing was performed to obtain snapshots of the plant and fungal transcriptome in mycorrhizal roots and in extraradical hyphae. To focus activities, the interactions between Medicago truncatula and Glomus intraradices as well as Populus tremula and Amanita muscaria were selected as models for AM and ECM symbioses, respectively. Together, almost, 20.000 expressed sequence tags (ESTs) were generated from different random and suppressive subtractive hybridization (SSH) cDNA libraries, providing a comprehensive overview of the mycorrhizal transcriptome. To automatically cluster and annotate EST-sequences, the BioMake and SAMS software tools were developed. In connection with the eNorthern software SteN, plant genes with a predicted mycorrhiza-induced expression were identified. To support experimental transcriptome profiling, macro- and microarray tools have been constructed for the two model mycorrhizae, based either on PCR-amplified cDNAs or 70mer oligonucleotides. These arrays were used to profile the transcriptome of AM and ECM roots under different conditions, and the data obtained were uploaded to the ArrayLIMS and EMMA databases that are designed to store and evaluate expression profiles from DNA arrays. Together, the EST- and transcriptome databases can be mined to identify candidate genes for targeted functional studies.


Assuntos
Biologia Computacional/métodos , Etiquetas de Sequências Expressas , Micorrizas/genética , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Simbiose/genética , Transcrição Gênica/genética
3.
Mol Plant Microbe Interact ; 18(8): 771-82, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16134889

RESUMO

The large majority of plants are capable of undergoing a tight symbiosis with arbuscular mycorrhizal (AM) fungi. During this symbiosis, highly specialized new structures called arbuscules are formed within the host cells, indicating that, during interaction with AM fungi, plants express AM-specific genetic programs. Despite increasing efforts, the number of genes known to be induced in the AM symbiosis is still low. In order to identify novel AM-induced genes which have not been listed before, 5,646 expressed sequence tags (ESTs) were generated from two Medicago truncatula cDNA libraries: a random cDNA library (MtAmp) and a suppression subtractive hybridization (SSH) library (MtGim), the latter being designed to enhance the cloning of mycorrhiza-upregulated genes. In silico expression analysis was applied to identify those tentative consensus sequences (TCs) of The Institute for Genomic Research M. truncatula gene index (MtGI) that are composed exclusively of ESTs deriving from the MtGim or MtAmp library, but not from any other cDNA library of the MtGI. This search revealed 115 MtAmp- or MTGim-specific TCs. For the majority of these TCs with sequence similarities to plant genes, the AM-specific expression was verified by quantitative reverse-transcription polymerase chain reaction. Annotation of the novel genes induced in mycorrhizal roots suggested their involvement in different transport as well as signaling processes and revealed a novel family of AM-specific lectin genes. The expression of reporter gene fusions in transgenic roots revealed an arbuscule-related expression of two members of the lectin gene family, indicating a role for AM-specific lectins during arbuscule formation or functioning.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas/genética , Lectinas/genética , Medicago truncatula/genética , Medicago truncatula/metabolismo , Micorrizas/metabolismo , Transcrição Gênica/genética , Sequência de Aminoácidos , Análise por Conglomerados , Etiquetas de Sequências Expressas , Genes de Plantas/genética , Lectinas/química , Lectinas/metabolismo , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Simbiose
4.
J Exp Bot ; 56(413): 799-806, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15668224

RESUMO

In this study the further characterization of the Vicia faba leghaemoglobin promoter pVfLb29 is presented that was previously shown to be specifically active in the infected cells of root nodules and in arbuscule-containing cells of mycorrhizal roots. Using promoter studies in transgenic hairy roots of the Pisum sativum mutant RisNod24, disabled in the formation of functional arbuscules, VfLb29 promoter activity is assigned to later stages of arbuscule development. In order to narrow down the regions containing cis-acting elements of pVfLb29, the activity of five VfLb29 promoter deletions (-797/-31 to -175/-31 in relation to the start codon) fused to the gusAint coding region were tested in transgenic V. hirsuta hairy roots. The results specify a promoter region ranging from position -410 to -326 (85 bp) as necessary for gus expression in arbuscule-containing cells, whereas this segment is not involved in the nodule-specific activity. Sequence analysis of the pVfLb29 fragment -410/-326 (85 bp) revealed sequence motifs previously shown to be cis-acting elements of diverse promoters. To investigate the autonomous function of pVfLb29 regions for activation in arbuscule-containing cells, different regions of pVfLb29 from positions -410 to -198 were used to prepare chimeric promoter constructs for trans-activation studies. These fragments alone did not activate the mycorrhiza inactive promoter of the Vicia faba leghaemoglobin gene VfLb3, showing that the activation of pVfLb29 in arbuscule-containing cells is governed by a complex regulatory system that requires at least two modules located between position -410 and -31 of the VfLb29 gene.


Assuntos
Leghemoglobina/genética , Micorrizas/fisiologia , Raízes de Plantas/fisiologia , Regiões Promotoras Genéticas/fisiologia , Sequência de Bases , Mapeamento Cromossômico , Regulação da Expressão Gênica de Plantas , Dados de Sequência Molecular , Pisum sativum/genética , Pisum sativum/fisiologia , Raízes de Plantas/microbiologia , Plantas Geneticamente Modificadas , Vicia/genética , Vicia faba/genética
5.
Mol Plant Microbe Interact ; 17(10): 1063-77, 2004 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15497399

RESUMO

Transcriptome profiling based on cDNA array hybridizations and in silico screening was used to identify Medicago truncatula genes induced in both root nodules and arbuscular mycorrhiza (AM). By array hybridizations, we detected several hundred genes that were upregulated in the root nodule and the AM symbiosis, respectively, with a total of 75 genes being induced during both interactions. The second approach based on in silico data mining yielded several hundred additional candidate genes with a predicted symbiosis-enhanced expression. A subset of the genes identified by either expression profiling tool was subjected to quantitative real-time reverse-transcription polymerase chain reaction for a verification of their symbiosis-induced expression. That way, induction in root nodules and AM was confirmed for 26 genes, most of them being reported as symbiosis-induced for the first time. In addition to delivering a number of novel symbiosis-induced genes, our approach identified several genes that were induced in only one of the two root endosymbioses. The spatial expression patterns of two symbiosis-induced genes encoding an annexin and a beta-tubulin were characterized in transgenic roots using promoter-reporter gene fusions.


Assuntos
Genes de Plantas , Medicago truncatula/genética , Medicago truncatula/microbiologia , Micorrizas/genética , Raízes de Plantas/genética , Raízes de Plantas/microbiologia , Anexinas/genética , Fungos/crescimento & desenvolvimento , Perfilação da Expressão Gênica , Análise de Sequência com Séries de Oligonucleotídeos , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Simbiose , Tubulina (Proteína)/genética
6.
Mol Plant Microbe Interact ; 16(10): 903-15, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14558692

RESUMO

The MtSucS1 gene encodes a sucrose synthase (EC 2.4.1.13) in the model legume Medicago truncatula. To determine the expression pattern of this gene in different organs and in particular during root endosymbioses, we transformed M. truncatula with specific regions of MtSucS1 fused to the gusAint reporter gene. These fusions directed an induction to the vasculature of leaves, stems, and roots as well as to flowers, developing seeds, young pods, and germinating seedlings. In root nodules, strong promoter activity occurred in the infected cells of the nitrogen-fixing zone but was additionally observed in the meristematic region, the prefixing zone, and the inner cortex, including the vasculature. Concerning endomycorrhizal roots, the MtSucS1 promoter mediated strongest expression in cortical cells harboring arbuscules. Specifically in highly colonized root sections, GUS-staining was furthermore detected in the surrounding cortical cells, irrespective of a direct contact with fungal structures. In accordance with the presence of an orthologous PsSus1 gene, we observed a comparable regulation of MtSucS1 expression in the grain legume Pisum sativum in response to microbial symbionts. Unlike other members of the MtSucS gene family, the presence of rhizobial or Glomus microsymbionts significantly altered and enhanced MtSucS1 gene expression, leading us to propose that MtSucS1 is involved in generating sink-strength, not only in root nodules but also in mycorrhizal roots.


Assuntos
Glucosiltransferases/genética , Medicago/genética , Medicago/microbiologia , Sequência de Bases , DNA de Plantas/genética , Éxons , Fungos/fisiologia , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Genes Reporter , Medicago/enzimologia , Dados de Sequência Molecular , Família Multigênica , Pisum sativum/enzimologia , Pisum sativum/genética , Pisum sativum/microbiologia , Filogenia , Raízes de Plantas/enzimologia , Raízes de Plantas/microbiologia , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Simbiose
7.
Mol Plant Microbe Interact ; 16(4): 306-14, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12744459

RESUMO

Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant. Until now, little was known about transcriptional changes which occur AM-exclusively; that is, they do not occur during other root-microbe associations, nor are they induced by improved phosphate nutrition. In order to identify such AM-exclusive gene inductions of Medicago truncatula, we used a pool of different RNA samples as subtractor population in a suppressive subtractive hybridization (SSH) experiment. This approach resulted in the identification of a number of new AM-regulated genes. None of these genes were expressed in nonmycorrhiza roots or leaves. Electronic data obtained by comparison of the cDNA sequences to expressed sequence tag (EST) sequences from a wide range of cDNA libraries in the M. truncatula EST database (Gene Index, MtGI) support the mycorrhiza specificity of the corresponding genes, because sequences in the MtGI that were found to match the identified SSH-cDNA sequences originated exclusively from AM cDNA libraries. The promoter of one of those genes, MtGst1, showing similarities to plant glutathione-S-transferase (GST) encoding genes, was cloned and used in reporter gene studies. In contrast to studies with the potato GST gene PRP, MtGst 1 promoter activity was detected in all zones of the root cortex colonized by Glomus intraradices, but nowhere else.


Assuntos
Regulação da Expressão Gênica de Plantas , Medicago/genética , Micorrizas/fisiologia , Transcrição Gênica , Sequência de Bases , Primers do DNA , Medicago/microbiologia , Medicago/fisiologia , Dados de Sequência Molecular , Micorrizas/crescimento & desenvolvimento , Raízes de Plantas/fisiologia , Reação em Cadeia da Polimerase , RNA de Plantas/genética , RNA de Plantas/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Simbiose
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