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1.
ACS Omega ; 6(41): 26880-26887, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34693109

RESUMO

The electrochemical behavior of magnetite (Fe3O4) aggregates with submicrometric size is investigated. Specifically, cyclic voltammetry tests were performed in both acidic (pH ∼ 4.5) and alkaline (pH ∼ 12.8) solutions, exploiting a conventional three-electrode cell. In the first case, the working electrode was made of a glassy carbon substrate loaded with magnetite nanoaggregates, forming a continuous film. In a second configuration, magnetite nanoaggregates were dispersed in solution, kept under stirring, as a fluidized electrode. The latter approach showed an increase in the electrochemical response of the particles, otherwise limited by the reduced active area as in the former case. Electrochemical-atomic force microscopy (EC-AFM) investigation was carried out in an acidic environment, showing the topography evolution of nanoaggregates during the electrochemical characterization. X-ray diffraction (XRD) analysis was carried out to evaluate the microstructural variation in the Fe3O4 electrodes after cathodic polarization tests in an alkaline environment.

2.
Nanotechnology ; 24(24): 245603, 2013 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-23690139

RESUMO

Efficient application of stem cells to the treatment of neurodegenerative diseases requires safe cell tracking to follow stem cell fate over time in the host environment after transplantation. In this work, for the first time, fluorescent and biocompatible methyl methacrylate (MMA)-based nanoparticles (fluoNPs) were synthesized through a free-radical co-polymerization process with a fluorescent macromonomer obtained by linking Rhodamine B and hydroxyethyl methacrylate. We demonstrate that the fluoNPs produced by polymerization of MMA-Rhodamine complexes (1) were efficient for the labeling and tracking of multipotent human amniotic fluid cells (hAFCs); (2) did not alter the main biological features of hAFCs (such as viability, cell growth and metabolic activity); (3) enabled us to determine the longitudinal bio-distribution of hAFCs in different brain areas after graft in the brain ventricles of healthy mice by a direct fluorescence-based technique. The reliability of our approach was furthermore confirmed by magnetic resonance imaging analyses, carried out by incubating hAFCs with both superparamagnetic iron oxide nanoparticles and fluoNPs. Our data suggest that these finely tunable and biocompatible fluoNPs can be exploited for the longitudinal tracking of stem cells.


Assuntos
Materiais Biocompatíveis/farmacologia , Rastreamento de Células/métodos , Nanopartículas/química , Células-Tronco/citologia , Animais , Biomarcadores/metabolismo , Endocitose/efeitos dos fármacos , Citometria de Fluxo , Fluorescência , Corantes Fluorescentes/química , Humanos , Implantes Experimentais , Imageamento por Ressonância Magnética , Camundongos , Microscopia Confocal , Nanopartículas/ultraestrutura , Coloração e Rotulagem , Transplante de Células-Tronco , Células-Tronco/efeitos dos fármacos , Células-Tronco/metabolismo , Imagem com Lapso de Tempo
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