RESUMO
A collaborative study was undertaken to determine the relationship between the three DNA repair complementation groups in xeroderma pigmentosum found at Erasmus University, Rotterdam, and the four groups found at the National Institutes of Health, Bethesda. The results of this study reveal that there are five currently known complementation groups in xeroderma pigmentosum.
Assuntos
Reparo do DNA , Teste de Complementação Genética , Xeroderma Pigmentoso/genética , Fusão Celular , Linhagem Celular , DNA/biossíntese , Teste de Complementação Genética/métodos , Humanos , National Institutes of Health (U.S.) , Hibridização de Ácido Nucleico , Recombinação Genética , Pele/metabolismo , Terminologia como Assunto , Estados Unidos , Xeroderma Pigmentoso/classificação , Xeroderma Pigmentoso/metabolismoRESUMO
Fibroblast strains from 12 patients with xeroderma pigmentosum had lower than normal rates of DNA repair, as determined by autoradiographic studies of ultraviolet-induced unscheduled nuclear DNA synthesis. The nuclei in binuclear cells, obtained by fusing fibroblasts from certain pairs of these strains, had a greater rate of DNA repair than the nuclei of either strain's unfused mononuclear cells. These results indicate that complementary corrections of the strains' repair defects had occurred in the fused cells. Four complementation groups were found, indicating that at least four mutations caused decreased DNA repair among these 12 strains. The unfused mononuclear cells of each group had a characteristic rate of repair that differed from the rates of the other groups.
