1.
J Chromatogr
; 500: 585-93, 1990 Feb 02.
Artigo
em Inglês
| MEDLINE
| ID: mdl-2329151
RESUMO
A biospecific sorbent for the isolation of ovalbumin antibodies was prepared by coupling of ovalbumin via its periodate-oxidized carbohydrate moiety to bead cellulose modified with adipic acid dihydrazide. The anti-ovalbumin IgG fraction isolated on this sorbent from immune rabbit serum contained only antibodies against protein determinants of ovalbumin. Thus, when these IgG were immobilized through their carbohydrate moieties to cellulose beads it became possible to prepare a biospecific sorbent for concanavalin A by oriented adsorption of ovalbumin. Ovalbumin was specifically adsorbed via its protein moiety and its carbohydrate part remained free for interaction with concanavalin A.
Assuntos
Anticorpos/isolamento & purificação , Carboidratos , Concanavalina A/isolamento & purificação , Imunoglobulina G/isolamento & purificação , Ovalbumina , Adsorção , Animais , Cromatografia de Afinidade , Concanavalina A/análise , Soros Imunes , Masculino , Ovalbumina/imunologia , Ovalbumina/isolamento & purificação , Coelhos
2.
Akush Ginekol (Sofiia)
; 16(3): 210-3, 1977.
Artigo
em Búlgaro
| MEDLINE
| ID: mdl-900426
3.
Nahrung
; 15(3): 289-94, 1971.
Artigo
em Alemão
| MEDLINE
| ID: mdl-5172902