RESUMO
The evolution of the actin cytoskeleton after trypsinization and recultivation as well as the effect of the PGE2 modulator and that of the secondary messenger, the cyclic AMP upon the same cytoskeletal proteins in human pulmonary fibroblasts (ICP-23) were studied. The substances were administered simultaneously and after one hour of viral adsorption. Using epifluorescence for pointing out filamentous actin the modifications occurring in this cytoskeletal protein when contacting trypsin and the virus and when PGE2 and cAMP are administered in the experimental variants are observed. Actin arrangement is obviously modified by the viral infection but the restrictive effect of PGE2 and cAMP upon virus replication is correlated with modifications occurring in the actin cytoskeleton.
Assuntos
Actinas/efeitos dos fármacos , AMP Cíclico/farmacologia , Citoesqueleto/efeitos dos fármacos , Dinoprostona/farmacologia , Pulmão/efeitos dos fármacos , Sarampo/microbiologia , Linhagem Celular , Células Cultivadas/efeitos dos fármacos , Células Cultivadas/microbiologia , Efeito Citopatogênico Viral/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fibroblastos/microbiologia , Humanos , Pulmão/citologia , Pulmão/microbiologia , Vírus do Sarampo/efeitos dos fármacos , Vírus do Sarampo/fisiologia , Fatores de Tempo , Tripsina/farmacologia , Replicação Viral/efeitos dos fármacosRESUMO
Viral suspensions of herpetic virus were inoculated on different cell cultures: human diploid cells (HDC) and monkey kidney cells--primary culture and cell line--for adapting the virus on these cells and obtaining several high constant titers. The human diploid cells and the monkey kidney cell line have proved to be the most suitable cells for the virus growth; thus an optimum development of the virus with a high constant titer was obtained starting with the 4th passage (10(5.7)-10(6)CPD/50/ml). The optimum conditions provided for the herpetic virus culture have allowed to obtain a herpetic virus stock used as control preparation for testing the efficacy of the "OFTALMOHERPIN" biological preparation for current use in the prophylaxis and therapy of ocular herpes.
Assuntos
Adaptação Fisiológica , Simplexvirus/fisiologia , Animais , Linhagem Celular , Células Cultivadas/microbiologia , Chlorocebus aethiops , Diploide , Humanos , Rim , Coelhos , Inoculações Seriadas , Simplexvirus/patogenicidade , Células Vero/microbiologia , Cultura de VírusRESUMO
A new simian embryo cell line has been established from a Cercopithecus male whole embryo. The resultant predominant fibroblast-like cell population has been frozen in convenient quantities and characterized for use in biological research as well as in theoretical and practical virological purposes. The cell line was designated ICCe-1 (Institute Cantacuzino Cercopithecus embryo-1).
