[Coagglutination test. Its improvement and use in the production of anatoxins]. / Reaktsiia koaggliutinatsii. Usovershenstvovanie i ispol'zovanie v proizvodstve anatoksinov.

The reactor technology for obtaining suspensions of Staphylococcus aureus (strain Cowan 1) with active protein A was developed. On the basis of the sensitization of bacterial suspensions with antitoxic rabbit sera staphylococcal reagents to diphtherial, gas gangrene (perfringens, oedematiens), botulinic toxins-toxoids were obtained and for the first time the coagglutination test with toxoids was carried out. More sensitive "specifically directed" reagents for the coagglutination test were obtained; for this purpose bacterial suspensions were sensitized with pure antibodies isolated from rabbit immune sera by immunosorption. The possibility of using the coagglutination test with specifically directed staphylococcal reagents for the rapid evaluation of the dynamics of toxin formation in reactors for the titration of purified toxoids, for the control of the purification of toxoids by ultrafiltration, as well as for the evaluation of the sorption-desorption of toxoids in the production of the corresponding preparations, was shown.