RESUMO
OBJECTIVE: Chronic Helicobacter pylori (H. pylori) infection promotes non-cardia gastric cancer. Some mouse models suggest that bone marrow derived cells (BMDC) contribute to Helicobacter-associated gastric carcinogenesis. We determined whether this increased susceptibility to Helicobacter-induced gastric carcinogenesis of p27-deficient mice is dependent upon their p27-null BMDC or their p27-null gastric epithelial cells. DESIGN: Female mice (recipients) were irradiated and transplanted with BMDC from male donors. Wild type (WT) mice in group 1 (control) received BMDC from male GFP-transgenic mice. Female WT and p27 KO mice were engrafted with male p27KO mice BMDC (Group 2) or GFP-transgenic WT BMDC (Group 3). Recipients were infected with H. pylori SS1 for one year. RESULTS: Mice lacking p27 in either the BM pool or gastric epithelium developed significantly more advanced gastric pathology, including high-grade dysplasia. Co-staining of donor BMDC in dysplastic gastric glands was confirmed by immunofluorescence. Gastric expression of IL-1 beta protein was reduced in groups 2 and 3 (p < 0.05 vs control) whereas expression of IFN-γ and chemokines MIP-1 beta, MIG, IP-10 and RANTES in group 2 were significantly higher than group 3. CONCLUSIONS: Both bone marrow-derived and gastric epithelial cells contribute to the increased gastric cancer susceptibility of p27-deficient H. pylori-infected mice.
Assuntos
Células da Medula Óssea/metabolismo , Inibidor de Quinase Dependente de Ciclina p27/deficiência , Infecções por Helicobacter/metabolismo , Neoplasias Gástricas/metabolismo , Animais , Células da Medula Óssea/microbiologia , Transplante de Medula Óssea/métodos , Inibidor de Quinase Dependente de Ciclina p27/genética , Citocinas/metabolismo , Células Epiteliais/metabolismo , Células Epiteliais/microbiologia , Células Epiteliais/patologia , Feminino , Mucosa Gástrica/metabolismo , Mucosa Gástrica/microbiologia , Mucosa Gástrica/patologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/fisiologia , Humanos , Masculino , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Mosaicismo , Neoplasias Gástricas/microbiologiaRESUMO
Bristle positions in two rows of bristles on the basitarsus of the second leg of the fruitfly Drosophila melanogaster were analyzed in order to determine the accuracy of bristle placement within these rows. Within each row the positions of the two terminal bristles were found to be approximately equally variable, and positional variability was found to increase toward the middle of each row. Rows having fewer bristles manifested more positional variability in their midsection. These results are interpreted in terms of a possible bristle spacing mechanism involving repulsive forces between mobile bristle cells.
