Dammarane-type triterpenoids from Gynostemma compressum X. X. Chen & D. R. Liang (Cucurbitaceae) and their AMPK activation effect in 3T3-L1 cells.

Bioassay-guided fractionation of the 80% ethanol extract of Gynostemma compressum X. X. Chen & D. R. Liang (Cucurbitaceae) resulted in the isolation and identification of eight undescribed triterpenoids, gycomol VN1, gycomol VN2, and gycomosides VN1-6 from the bioactive n-butanol fraction. The structures of these compounds were elucidated by one- and two-dimensional nuclear magnetic resonance spectroscopy, high-resolution electrospray ionisation mass spectrometry, and chemical methods. All isolated compounds were evaluated for their 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-coenzyme A carboxylase (ACC) activation effects on 3T3-L1 cells. Importantly, gycomol VN2, gycomoside VN1, and gycomosides VN3-5 activated the phosphorylation of AMPK and its downstream substrate ACC in 3T3-L1 cells at a dose of 10 µM. These effects imply that the activation of AMPK and ACC by active compounds from G. compressum has considerable potential for the prevention of obesity and its related disorders by activating AMPK signaling pathways.

New dammarane-type triterpenoid glycosides from Gynostemma burmanicum.

The chemical composition of Gynostemma burmanicum King ex Chakrav. was investigated for the first time in this study. Nine dammarane glycosides (1‒9) were isolated from the EtOH extract of the aerial parts of G. burmanicum. Their structures were elucidated by 1D and 2D NMR spectroscopic interpretation as well as by chemical studies. The new compounds were 3ß,20S-dihydroxydammar-24-ene-3-O-ß-D-glucopyranosyl-20-O-[ß-D-xylopyranosyl-(1→6)-ß-D-glucopyranoside] (1), 3ß,12ß,20S-trihydroxydammar-24-ene-3-O-ß-D-xylopyranosyl-20-O-[ß-D-xylopyranosyl-(1→6)-ß-D-glucopyranoside] (2), and 12-oxo-3ß,20S-dihydroxydammar-24-ene-3-O-[ß-D-glucopyranosyl(1→2)-ß-D-glucopyranosyl]-20-O-[ß-D-xylopyranosyl-(1→6)-ß-D-glucopyranoside] (3).

Two new abietane diterpenes huperphlegmarins A and B from Huperzia phlegmaria.

Two new abietane diterpenes, huperphlegmarin A-B (1-2), were isolated from the aerial parts of Huperzia phlegmaria (L.) Rothm. (Lycopodiaceae), in addition to five known compounds including lycoxanthol (3), 21ß-hydroxyserrat-14-en-3ß-yl acetate (4), 21α-hydroxyserrat-14-en-3ß-yl acetate (5), 21α-hydroxyserrat-14-en-3ß-ol (6), and fawcettidine (7). Their structures were determined by the combination analyses of spectroscopy, including 1D-, 2D-NMR, HRESIMS, CD and comparison with the reported data in the literature. Huperphlegmarin B (2) presented the rare 1,11-epoxy group in the molecule. Compounds 3, 4, 5 and 6 were performed for AChE inhibitory activity and compound 3 showed the inhibitory activity against AChE with IC50 of 465.6 µg/mL.

Preliminary avaluation of the real situation of pharmacists graduated in Pharmacy College during their activities in the Delta of Mekong River

The research presents results of study about quantity and distribution real situation of pharmacists who are working at national health centers in Cuu Long area and initial evaluate training and working quality, retrain need and post graduate of pharmacists. Method: retrospective, cross- sectional description. Result: lack of pharrmacist at health center in Cuu Long area, working quality and speciality level depend on training center and suitability in using pharmacy manpower and training need

Studying of flavonoids in the leaves of Holarrhena antidysenterica Wall

The study on Holarrhena antidysenterica Wall was carried out in Chi Linh (Hai Duong province) in September 2004. Leaves of Holarrhena antidysenterica Wall were collected, dried, grilled to powder, then extracted and isolated flavonoid. Two flavonoids were isolated, on the base of m.p, UV, IR, MS, 1H-NMR, 13C-NMR, DEPT analyses, they were identified as Kaempferol and Quercetin. This is the first time the flavonoids were reported as a substance isolated from Holarrhena antidysenterica Wall in Vietnam

A survey on flavonoids of Sambucus L. species

There were 20 flavonoids isolated and identified from plants of genus Sambucus, including 8 anthocyanines and 12 other flavonoids. According to Richard Bolli, genus Sambucus worldwide included 9 species, 8 subspecies and 2 variety. Only 2 species and 2 subspecies among them were studied about flavonoid components, so it needs further studies of flavonoids in other plants of genus Sambucus, to find natural sources of antioxydative compounds for prevention and treatment

Chemical components of the branch of Uncaria Schreb

Index Kewensis lists 80 genera worldwide, while Tran Ngoc Ninh lists 60 genera of uncaria. In Vietnam, there are 3 genera of uncaria schreb “Common plants in Vietnam – Le Kha Ke” commonly used in traditional medicine for treating hypertension and the symtoms of dizzy, headache and insomnia