Dimercaprol-modified mesoporous silica nanoparticles for efficient removal of toxic mercury ions from aqueous solution.

A surface-modified mesoporous silica nanoparticle containing dimercaprol monomers was created utilizing the sol-gel condensation process, using tetraethyl orthosilicate (TEOS) as the silica source and poloxamer as the structure directing agent. To accomplish this synthesis, 3-glycidoxypropyl triethoxysilane (GPTS, 20 mol%) was incorporated into the silica walls during the sol-gel condensation process, along with TEOS. Furthermore, dimercaprol (DM) monomers were incorporated onto silica surfaces by a ring-opening reaction between GPTS epoxy groups, and dimercaprol hydroxyl groups. The prepared dimercaprol-modified silica adsorbent (MSN-DT NPs) material has been studied using a variety of instruments, including XRD, FT-IR, N2 adsorption-desorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric (TG) analysis, and zeta potential analysis. The MSN-DT NPs material selectively adsorbs mercury ions, with a high adsorption amount of 125 mg/g and a removal capability of roughly ~ 90% from the original metal ion mixture comprising other competing metals such as Pb2+, Ni2+, Fe2+, and Zn2+. The MSN-DT NPs adsorbent shows recyclable qualities for up to five cycles when treated with an acidic aqueous solution (0.1 M HCl). As a result, the MSN-DT NPs adsorbent may be regenerated and reused up to five times without losing its adsorption effectiveness. The experimental findings showed that the MSN-DT NPs adsorbent may be employed to selectively remove hazardous Hg2+ ions from an aqueous solution.

Synthesis of bis(2-aminoethyl)amine functionalized mesoporous silica (SBA-15) adsorbent for selective adsorption of Pb2+ ions from wastewater.

Rapid growth in the industry has released large quantities of contaminants, particularly metal discharges into the environment. Heavy metal poisoning in water bodies has become a major problem due to its toxicity to living organisms. In this study, we developed a 3-chloropropyl triethoxysilane incorporated mesoporous silica nanoparticle (SBA-15) based adsorbent utilizing the sol-gel process and Pluronic 123 (P123) as a structure-directing surfactant. Furthermore, the produced SBA-15 NPs were functionalized with bis(2-aminoethyl)amine (BDA) using the surface grafting approach. The physical and chemical properties of the prepared SBA-15@BDA NPs were determined using a variety of instruments, including small-angle X-ray diffraction (SAXS), Fourier-transform infrared (FTIR), scanning electron microscope (SEM), N2 adsorption-desorption, thermogravimetric, particle size distribution, and zeta potential analysis. The MSN has a large surface area of up to 574 m2/g, a pore volume of 0.57 cm3/g, and a well-ordered mesoporous nanostructure with an average pore size of 3.6 nm. The produced SBA-15@BDA NPs were used to adsorb selectively to lead (Pd2+) ions from an aqueous solution. The adsorption study was performed under various conditions, including the influence of solution pH, adsorbent dose, adsorption kinetics, adsorption selectivity in the presence of competing metal ions, and reusability. The results of the kinetic study demonstrated that SBA-15@BDA NPs absorb selectively Pb2+ ions via chemisorption. The SBA-15@BDA NPs show Pb2+ ions with a maximum adsorption capacity of ~ 88% and an adsorbed quantity of approximately ~ 112 mg/g from the studied aqueous solution. The adsorption mechanism relies on coordination bonding between Pb2+ ions and surface-functionalized amine groups on SBA-15@BDA NPs. Furthermore, the proposed SBA-15@BDA NPs adsorbent demonstrated excellent reusability over five cycles without significantly reducing adsorption performance. As a consequence, SBA-15@BDA NPs might serve as an effective adsorbent for the selective removal of Pb2+ ions from aqueous effluent.

pNIPAm-Based pH and Thermoresponsive Copolymer Hydrogel for Hydrophobic and Hydrophilic Drug Delivery.

The regulated and targeted administration of hydrophobic and hydrophilic drugs is both promising and challenging in the field of drug delivery. Developing a hydrogel which is responsive to dual stimuli is considered a promising and exciting research area of study. In this work, melamine functionalized poly-N-isopropyl acrylamide-co-glycidyl methacrylate copolymer has been developed by copolymerizing glycidyl methacrylate (GMA) monomer with N-isopropyl acrylamide (NIPAm) and further functionalized with melamine units (pNIPAm-co-pGMA-Mela). The prepared pNIPAm-co-pGMA-Mela copolymer hydrogel was characterized using various characterization techniques, including 1H NMR, FTIR, SEM, zeta potential, and particle size analysis. A hydrophobic drug (ibuprofen, Ibu) and hydrophilic drug (5-fluorouracil, 5-Fu) were selected as model drugs. Dual pH and temperature stimuli-responsive drug release behavior of the pNIPAm-co-pGMA-Mela hydrogel was evaluated under different pH (pH 7.4 and 4.0) and temperature (25 °C, 37 °C, and 45 °C) conditions. Furthermore, the in vitro biocompatibility of the developed pNIPAm-co-pGMA-Mela copolymer hydrogel was determined on MDA-MB-231 cells. The pH and temperature-responsive drug delivery study results reveal that the pNIPAm-co-pGMA-Mela hydrogel system is responsive to both pH and temperature stimuli and exhibits about ~100% of Ibu and 5-Fu, respectively, released at pH 4.0/45 °C. Moreover, the MTT assay and hemocompatibility analysis results proved that the pNIPAm-co-pGMA-Mela hydrogel system is biocompatible and hemocompatible, suggesting that that it could be used for drug delivery applications. The experimental results suggest that the proposed pNIPAm-co-pGMA-Mela hydrogel system is responsive to dual pH and temperature stimuli, and could be a promising drug carrier system for both hydrophilic and hydrophobic drug delivery applications.

k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.

The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites. The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work. First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG). Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups. Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system. Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system. Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (∼ 68 %) and enhanced drug release. Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible. As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.

L-lysine Functionalized Mesoporous Silica Hybrid Nanoparticles for pH-Responsive Delivery of Curcumin.

Stimuli-responsive controlled drug delivery systems have attracted the attention of researchers in recent decades due to their potential application in developing efficient drug carriers that are responsive to applied stimuli triggers. In this work, we present the synthesis of L-lysine (an amino acid that combines both amine and carboxylic acid groups in a single unit) modified mesoporous silica nanoparticles (MS@Lys NPs) for the delivery of the anticancer bioactive agent (curcumin, Cur) to cancer cells. To begin, mesoporous silica hybrid nanoparticles (MS@GPTS NPs) with 3-glycidoxypropyl trimethoxy silane (GPTS) were synthesized. The L-lysine groups were then functionalized onto the mesopore channel surfaces of the MS@GPTS NPs through a ring-opening reaction between the epoxy groups of the GPTS and the amine groups of the L-lysine units. Several instrumental techniques were used to examine the structural properties of the prepared L-lysine-modified mesoporous silica nanoparticles (MS@Lys NPs). The drug loading and pH-responsive drug delivery behavior of MS@Lys NPs were studied at different pH levels (pH 7.4, 6.5, and 4.0) using curcumin (Cur) as a model anticancer bioactive agent. The MS@Lys NPs' in vitro cytocompatibility and cell uptake behavior were also examined using MDA-MB-231 cells. The experimental results imply that MS@Lys NPs might be used in cancer therapy as pH-responsive drug delivery applications.

L-Lysine-Modified pNIPAm-co-GMA Copolymer Hydrogel for pH- and Temperature-Responsive Drug Delivery and Fluorescence Imaging Applications.

The development of dual-stimuli-responsive hydrogels attracts much research interest owing to its unique stimuli-responsive characteristics. In this study, a poly-N-isopropyl acrylamide-co-glycidyl methacrylate-based copolymer was synthesized by incorporating N-isopropyl acrylamide (NIPAm) and a glycidyl methacrylate (GMA) monomer. The synthesized copolymer, pNIPAm-co-GMA was further modified with L-lysine (Lys) functional units and further conjugated with fluorescent isothiocyanate (FITC) to produce a fluorescent copolymer pNIPAAm-co-GMA-Lys hydrogel (HG). The in vitro drug loading and dual pH- and temperature-stimuli-responsive drug release behavior of the pNIPAAm-co-GMA-Lys HG was investigated at different pH (pH 7.4, 6.2, and 4.0) and temperature (25 °C, 37 °C, and 45 °C) conditions, respectively, using curcumin (Cur) as a model anticancer drug. The Cur drug-loaded pNIPAAm-co-GMA-Lys/Cur HG showed a relatively slow drug release behavior at a physiological pH (pH 7.4) and low temperature (25 °C) condition, whereas enhanced drug release was achieved at acidic pH (pH 6.2 and 4.0) and higher temperature (37 °C and 45 °C) conditions. Furthermore, the in vitro biocompatibility and intracellular fluorescence imaging were examined using the MDA-MB-231 cell line. Therefore, we demonstrate that the synthesized pNIPAAm-co-GMA-Lys HG system with temperature- and pH-stimuli-responsive features could be promising for various applications in biomedical fields, including drug delivery, gene delivery, tissue engineering, diagnosis, antibacterial/antifouling material, and implantable devices.

Thermosensitive Polymer-Modified Mesoporous Silica for pH and Temperature-Responsive Drug Delivery.

A mesoporous silica-based drug delivery system (MS@PNIPAm-PAAm NPs) was synthesized by conjugating the PNIPAm-PAAm copolymer onto the mesoporous silica (MS) surface as a gatekeeper that responds to temperature and pH changes. The drug delivery studies are carried out in vitro at different pH (7.4, 6.5, and 5.0) and temperatures (such as 25 °C and 42 °C, respectively). The surface conjugated copolymer (PNIPAm-PAAm) acts as a gatekeeper below the lower critical solution temperature (LCST) (<32 °C) and as a collapsed globule structure above LCST (>32 °C), resulting in controlled drug delivery from the MS@PNIPAm-PAAm system. Furthermore, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cellular internalization results support the prepared MS@PNIPAm-PAAm NPs being biocompatible and readily taken up by MDA-MB-231 cells. The prepared MS@PNIPAm-PAAm NPs, with their pH-responsive drug release behavior and good biocompatibility, could be used as a drug delivery vehicle where sustained drug release at higher temperatures is required.

Selective removal of copper (II) ions from aqueous solution using pyridyl-bridged mesoporous organosilica hybrid adsorbent.

The incorporation of active functional groups into the mesoporous organosilica hybrid materials is efficient for various applications. A newly designed mesoporous organosilica adsorbent was prepared using diaminopyridyl groups bridged-(bis-trimethoxy)organosilane (DAPy) precursor by using Pluronic P123 as structure directing template via sol-gel co-condensation method. The hydrolysis reaction of DAPy precursor and tetraethyl orthosilacate (TEOS) with a DAPy content of about 20 mol% to TEOS were incorporated into the mesopore walls of the mesoporous organosilica hybrid nanoparticles (DAPy@MSA NPs). Low-angle XRD and FT-IR, N2 adsorption-desorption analysis, SEM, TEM, and TG analysis were used to characterize the synthesized DAPy@MSA NPs. The DAPy@MSA NPs exhibit an order mesoporous structure with a high surface area, mesopore size and pore volume of approximately ∼465 m2/g, 4.4 nm and 0.48 cm3/g, respectively. The pyridyl groups integrated DAPy@MSA NPs showed the selective adsorption of Cu2+ ions from the aqueous medium by metal-ligand complex coordination of Cu2+ ions with the integrated pyridyl groups and the pendant hydroxyl (-OH) functional groups present into the mesopore walls of the DAPy@MSA NPs. In the presence of other competitive metal ions (Cr2+, Cd2+, Ni2+, Zn2+, and Fe2+), the DAPy@MSA NPs showed relatively high adsorption of Cu2+ ions (276 mg/g) from aqueous solution as compared to the other competitive metal ions at the same concentration (100 mg/L) of initial metal ion solution.

Efficient label-free in vivo photoacoustic imaging of melanoma cells using a condensed NIR-I spectral window.

In this paper, we propose an efficient label-free in vivo photoacoustic (PA) imaging of melanoma using a condensed near infrared-I (NIR-I) supercontinuum light source. Although NIR-II spectral window is advantageous such as longer penetration depth compared to the NIR-I region, supercontinuum light sources emitting both NIR-I and NIR-II region could lower the efficiency to target melanoma because of low optical power density in the melanoma's absorption spectra. To exploit efficient in vivo PA imaging of melanoma, we demonstrated the light source emitting from visible (532-600 nm) to NIR-I (600-1000 nm) by optimizing stimulated Raman scattering induced supercontinuum generation. The melanoma's structure is successfully differentiated from blood vessels at a high pulse energy of 2.5 µJ and a flexible pulse repetition rate (PRR) of 5-50 kHz. The proposed light source with the microjoules energies and tens of kHz of PRR can potentially accelerate clinical trials such as early diagnosis of melanoma.

Gating Mechanism of the Voltage-Gated Proton Channel Studied by Molecular Dynamics Simulations.

The voltage-gated proton channel Hv1 has important roles in proton extrusion, pH homeostasis, sperm motility, and cancer progression. The Hv1 channel has also been found to be highly expressed in cell lines and tissue samples from patients with breast cancer. A high-resolution closed-state structure has been reported for the mouse Hv1 chimera channel (mHv1cc), solved by X-ray crystallography, but the open-state structure of Hv1 has not been solved. Since Hv1 is a promising drug target, various groups have proposed open conformations by molecular modeling and simulation studies. However, the gating mechanism and the open-state conformation under the membrane potential are still debate. Here, we present a molecular dynamics study considering membrane potential and pH conditions. The closed-state structure of mHv1cc was used to run molecular dynamics (MD) simulations with respect to electric field and pH conditions in order to investigate the mechanism of proton transfer. We observed a continuous hydrogen bond chain of water molecules called a water-wire to be formed through the channel pore in the channel opening, triggered by downward displacement of the S2 helix and upward movement of the S4 helix relative to other helices. Due to the movement of the S2 and S4 helices, the internal salt bridge network was rearranged, and the hydrophobic gating layers were destroyed. In line with previous experimental and simulation observations, our simulation results led us to propose a new gating mechanism for the Hv1 proton channel, and may provide valuable information for novel drug discovery.

pH and Thermoresponsive PNIPAm-co-Polyacrylamide Hydrogel for Dual Stimuli-Responsive Controlled Drug Delivery.

The therapeutic delivery system with dual stimuli-responsiveness has attracted attention for drug delivery to target sites. In this study, we used free radical polymerization to develop a temperature and pH-responsive poly(N-isopropyl acrylamide)-co-poly(acrylamide) (PNIPAM-co-PAAm). PNIPAm-co-PAAm copolymer by reacting with N-isopropyl acrylamide (NIPAm) and acrylamide (Am) monomers. In addition, the synthesized melamine-glutaraldehyde (Mela-Glu) precursor was used as a cross-linker in the production of the melamine cross-linked PNIPAm-co-PAAm copolymer hydrogel (PNIPAm-co-PAAm-Mela HG) system. The temperature-responsive phase transition characteristics of the resulting PNIPAM-co-PAAm-Mela HG systems were determined. Furthermore, the pH-responsive drug release efficiency of curcumin was investigated under various pH and temperature circumstances. Under the combined pH and temperature stimuli (pH 5.0/45 °C), the PNIPAm-co-PAAm-Mela HG demonstrated substantial drug loading (74%), and nearly complete release of the loaded drug was accomplished in 8 h. Furthermore, the cytocompatibility of the PNIPAm-co-PAAm-Mela HG was evaluated on a human liver cancer cell line (HepG2), and the findings demonstrated that the prepared PNIPAm-co-PAAm-Mela HG is biocompatible. As a result, the PNIPAm-co-PAAm-Mela HG system might be used for both pH and temperature-stimuli-responsive drug delivery.

Update on Chitosan-Based Hydrogels: Preparation, Characterization, and Its Antimicrobial and Antibiofilm Applications.

Chitosan is a prominent biopolymer in research for of its physicochemical properties and uses. Each year, the number of publications based on chitosan and its derivatives increases. Because of its comprehensive biological properties, including antibacterial, antioxidant, and tissue regeneration activities, chitosan and its derivatives can be used to prevent and treat soft tissue diseases. Furthermore, chitosan can be employed as a nanocarrier for therapeutic drug delivery. In this review, we will first discuss chitosan and chitosan-based hydrogel polymers. The structure, functionality, and physicochemical characteristics of chitosan-based hydrogels are addressed. Second, a variety of characterization approaches were used to analyze and validate the physicochemical characteristics of chitosan-based hydrogel materials. Finally, we discuss the antibacterial, antibiofilm, and antifungal uses of supramolecular chitosan-based hydrogels. This review study can be used as a base for future research into the production of various types of chitosan-based hydrogels in the antibacterial and antifungal fields.

Erratum: Phan, T.T.V., et al. Spontaneous Hinge-Bending Motions of Angiotensin I Converting Enzyme: Role in Activation and Inhibition. Molecules 2020, 25, 1288.

The authors wish to make the following corrections to the paper [...].

Roles of Chitosan in Green Synthesis of Metal Nanoparticles for Biomedical Applications.

Chitosan (CS) is a well-known stabilizer for metal nanoparticles in biomedical engineering. However, very few studies have explored other important roles of CS including reducing, shape-directing, and size-controlling. This review aims to provide the latest and most comprehensive overview of the roles of CS in the green synthesis of metal nanoparticles for biomedical applications. To the best of our knowledge, this is the first review that highlights these potentialities of CS. At first, a brief overview of the properties and the bioactivity of CS is presented. Next, the benefits of CS for enhancing the physicochemical behaviors of metal nanoparticles are discussed in detail. The representative biomedical applications of CS-metal nanoparticles are also given. Lastly, the review outlines the perceptual vision for the future development of CS-metal nanoparticles in the biomedicine field.

A portable device with low-power consumption for monitoring mouse vital signs during in vivo photoacoustic imaging and photothermal therapy.

OBJECTIVE: The aim of this study was to monitor the physiological changes and cytotoxic effects of exogenous contrast agents during photoacoustic imaging (PAI) and photothermal therapy (PTT). In this paper, a low-power telemetric device for mouse vital signs monitoring was designed and demonstrated. APPROACH: The power consumption was optimized through hardware and software co-design with a 17% increased operating time compared with typical operation. To demonstrate the feasibility of the monitoring device, PAI and PTT experiments with chitosan-polypyrrole nanocomposites (CS-PPy NCs) as exogenous contrast agents were conducted. Herein, the physiological variation in groups of mice with different CS-PPy NC concentrations was observed and analyzed. MAIN RESULTS: The experimental results indicated the influence of CS-PPy NCs and anesthesia on mouse vital signs in PAI and PTT. Additionally, the association between core temperature, heart rate, and saturation of peripheral oxygen (SpO2) during PAI and PTT was shown. The strong near-infrared absorbance of exogenous contrast agents could account for the increase in mouse core temperature and tumor temperature in this study. Furthermore, high cross-correlation values between core temperature, heart rate, and SpO2 were demonstrated to explain the fluctuation of mouse vital signs during PAI and PTT. SIGNIFICANCE: A design of a vital signs monitoring device, with low power consumption, was introduced in this study. A high cross correlation coefficient of mouse vital signs and the effects of CS-PPy NCs were observed, which explained the mouse physiological variation during the PAI and PTT experiments.

Photothermal Responsive Porous Membrane for Treatment of Infected Wound.

Wound infection is a big issue of modern medicine because of multi-drug resistance bacteria; thus, developing an advanced therapy is curial. Photothermal therapy (PTT) is a newly noninvasive strategy that employs PTT agents to transfer near-infrared (NIR) light energy into heat to kill bacterial pathogens. In this work, the PTT agent-containing dressing was developed for the first time to treat the wound infection. Palladium nanoparticles (PdNPs) were chosen as PTT agents because of their high stability, good biocompatibility, excellent photothermal property, and simple-green preparation. With the flexibility and wettability, highly porous membrane chitosan/polyvinyl alcohol (CS/PVA) membrane was chosen as the dressing. The prepared wound dressings exhibited excellent biocompatibility, high porosity, a high degree of swelling, high moisture retention, and high photothermal performance. The treatment of PdNPs loading CS/PVA dressing (CS/PVA/Pd) and laser irradiation killed most of the bacteria in vitro. The proposed PTT agent containing wound dressing introduces a novel strategy for the treatment of wound infection.

Biofilm inhibition, modulation of virulence and motility properties by FeOOH nanoparticle in Pseudomonas aeruginosa.

Biofilm formation is one of the resistance mechanisms of Pseudomonas aeruginosa against antimicrobial compounds. Biofilm formation also characterizes for the infection and pathogenesis of P. aeruginosa, along with production of various virulence factors. With recent development of nanotechnology, the present study aims to employ the synthetic iron nanoparticle (FeOOH-NP) as an active agent to inhibit the formation of P. aeruginosa biofilm. The FeOOH-NP was synthesized and characterized with rod shape and average size of 40 nm. Inhibition of biofilm formation by the FeOOH-NP is in a concentration-dependent manner, with inhibition of biofilm formation increased as the FeOOH-NP concentration increased. Microscopic observations also confirmed the disruption of the biofilm architecture in the presence of the FeOOH-NP. In addition, the presence of the FeOOH-NP was also found to modulate bacterial motility as well as some other important virulence factors produced simultaneously with biofilm formation. These findings provide insights to anti-biofilm effect of a new iron NP, contributing to the search for an effective agent to combat P. aeruginosa infections resulted from biofilm formation.

Design, Fabrication, and Evaluation of Multifocal Point Transducer for High-Frequency Ultrasound Applications.

The present study illustrates the design, fabrication, and evaluation of a novel multifocal point (MFP) transducer based on polyvinylidene fluoride (PVDF) film for high-frequency ultrasound application. The fabricated MFP surface was press-focused using a computer numerical control (CNC) machining tool-customized multi-spherical pattern object. The multi-spherical pattern has five spherical surfaces with equal area and connected continuously to have the same energy level at focal points. Center points of these spheres are distributed in a linear pattern with 1 mm distance between each two points. The radius of these spheres increases steadily from 10 mm to 13.86 mm. The designed MFP transducer had a center frequency of 50 MHz and a -6 dB bandwidth of 68%. The wire phantom test was conducted to study and demonstrate the advantages of this novel design. The obtained results for MFP transducer revealed a significant increase (4.3 mm) of total focal zone in the near-field and far-field area compared with 0.48 mm obtained using the conventional single focal point transducer. Hence, the proposed method is promising to fabricate MFP transducers for deeper imaging depth applications.

An Up-To-Date Review on Biomedical Applications of Palladium Nanoparticles.

Palladium nanoparticles (PdNPs) have intrinsic features, such as brilliant catalytic, electronic, physical, mechanical, and optical properties, as well as diversity in shape and size. The initial researches proved that PdNPs have impressive potential for the development of novel photothermal agents, photoacoustic agents, antimicrobial/antitumor agents, gene/drug carriers, prodrug activators, and biosensors. However, very few studies have taken the benefit of the unique characteristics of PdNPs for applications in the biomedical field in comparison with other metals like gold, silver, or iron. Thus, this review aims to highlight the potential applications in the biomedical field of PdNPs. From that, the review provides the perceptual vision for the future development of PdNPs in this field.

Chitosan as a stabilizer and size-control agent for synthesis of porous flower-shaped palladium nanoparticles and their applications on photo-based therapies.

This study reported a newly developed green synthesis method using chitosan and vitamin C to prepare porous flower-shaped palladium nanoparticles. We found that chitosan not only worked as a stabilizer but also as a size-control agent for the synthesis of these nanoparticles. The growth model of flower-shaped palladium nanoparticles was proposed to interpret mechanistic understanding. The obtained nanoparticles showed good biocompatibility and strong near-infrared absorption. The nanoparticles were successfully demonstrated to be highly efficient for both in vitro photothermal therapy and in vitro photoacoustic imaging.