Disseminated Sporotrichosis With Brain Abscesses in an HIV-Infected Patient.

Sporotrichosis is a mycotic infection caused by the Sporothrix schenckii species complex. It typically presents as a localized, mostly cutaneous or lymphocutaneous infection, but has been increasingly presenting as a disseminated infection in immunocompromised individuals, especially in HIV/AIDS patients. We also included a literature review of sporotrichosis in this patient population. The aim of this case report is to raise awareness about atypical presentations of sporotrichosis in an attempt to decrease the time to diagnosis, initiate treatment earlier when infection is suspected, and improve overall survival in vulnerable patient populations.

HIV Care in Asian and Pacific Islanders in Kansas.

A complex cultural dynamic within the Asian and Pacific Islander (APIs) population contributes to barriers in HIV care. This qualitative narrative study investigated how awareness, resource accessibility, and cultural taboo impact HIV care in APIs in Kansas. Eleven HIV-infected API patients were interviewed. Two evaluators independently completed a qualitative themes analysis. Important themes impacting HIV care included lack of awareness, sex as a taboo topic, and misconceptions about HIV infection. These factors create a closed community regarding HIV prevention and care. Clinicians must be aware of these barriers and provide specific culturally sensitive information and care to this population.

Strength of Resistance to "Flip" the Tightened Half-Hitches of an Arthroscopic Knot.

PURPOSE: The purpose of this study was to biomechanically determine the minimum strength required to "flip" a seated and tightened half-hitch in a knot of different braided polyblend sutures. METHODS: ForceFiber, FiberWire, Orthocord, and Ultrabraid were evaluated. All knot tying processes began by advancing an initial base knot down to a standardized rod. All half-hitches were tied using a single-hole knot pusher in a dry environment, and were tightened with 45N by using past-pointing maneuver. A tension was then deliberately applied to the wrapping suture limb until a "flip" occurred, and the amount of tension was measured by the load cell. Three trials for each half-hitch and 3 half-hitches for each arthroscopic knot with 12 knots of each material were tied. All respective tension loads were collected through the digital video recordings (knot closeup view and load cell reading). RESULTS: Orthocord was the easiest to "flip" a seated and tightened half-hitch (average: 10±3N), whereas Ultrabraid was hardest to "flip" (average: 23±7N). ForceFiber and FiberWire were about 49% and 15% more resistance to "flip" when compared to Orthocord. After each trial of flipping the half-hitch back and re-tightened, there was a significant reduction in strength required to "flip" the tightened half-hitch. CONCLUSION: 1) The unintentional minimum tensile strength greater than 10N applied to the wrapping suture limb on the seated and tightened half-hitch could potentially "flip" the half-hitch in a knot during arthroscopic procedures, and 2) different braided suture material has an affect on the tensile strength to "flip" a seated and tightened half-hitch in an arthroscopic knot. CLINICAL RELEVANCE: The findings of this study indicated that even a seated and tightened half-hitch in an arthroscopic knot could easily be "flipped" if an unintentional tension was applied to the wrapping suture limb, and thereby reduced the knot security strength. In addition different suture materials could have an effect on the strength needed to "flip" the tightened half-hitch.

Nanoporous impedemetric biosensor for detection of trace atrazine from water samples.

Trace contamination of ground water sources has been a problem ever since the introduction of high-soil-mobility pesticides, one such example is atrazine. In this paper we present a novel nanoporous portable bio-sensing device that can identify trace contamination of atrazine through a label-free assay. We have designed a pesticide sensor comprising of a nanoporous alumina membrane integrated with printed circuit board platform. Nanoporous alumina in the biosensor device generates a high density array of nanoscale confined spaces. By leveraging the size based immobilization of atrazine small molecules we have designed electrochemical impedance spectroscopy based biosensor to detect trace amounts of atrazine. We have calibrated the sensor using phosphate buffered saline and demonstrated trace detection from river and bottled drinking water samples. The limit of detection in all the three cases was in the femtogram/mL (fg/mL) (parts-per-trillion) regime with a dynamic range of detection spanning from 10 fg/mL to 1 ng/mL (0.01 ppt to 1 ppm). The selectivity of the device was tested using a competing pesticide; malathion and selectivity in detection was observed in the fg/mL regime in all the three cases.