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1.
Mar Drugs ; 19(7)2021 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-34202376

RESUMO

The aims of this work were to evaluate the contribution of the free fatty acid (FA) pool to triacylglyceride (TAG) biosynthesis and to try to characterize the mechanism by which FA are assimilated into TAG in the green alga Dunaliella tertiolecta. A time-resolved lipidomic analysis showed that nitrogen (N) deprivation induces a redistribution of total lipidome, particularly of free FA and major polar lipid (PL), in parallel to enhanced accumulation of polyunsaturated TAG. The steady-state concentration of the FA pool, measured by prolonged 14C-bicarbonate pre-labeling, showed that N deprivation induced a 50% decrease in total FA level within the first 24 h and up to 85% after 96 h. The abundance of oleic acid increased from 50 to 70% of total free FA while polyunsaturated FA (PUFA) disappeared under N deprivation. The FA flux, measured by the rate of incorporation of 14C-palmitic acid (PlA), suggests partial suppression of phosphatidylcholine (PC) acyl editing and an enhanced turnover of the FA pool and of total digalactosyl-diacylglycerol (DGDG) during N deprivation. Taken together, these results imply that FA biosynthesis is a major rate-controlling stage in TAG biosynthesis in D. tertiolecta and that acyl transfer through PL such as PC and DGDG is the major FA assimilation pathway into TAG in that alga and possibly in other green microalgae. Increasing the availability of FA could lead to enhanced TAG biosynthesis and to improved production of high-value products from microalgae.


Assuntos
Clorófitas , Ácidos Graxos/metabolismo , Microalgas , Triglicerídeos/metabolismo , Animais , Organismos Aquáticos , Humanos , Metabolismo dos Lipídeos , Nitrogênio/metabolismo
2.
Planta ; 249(1): 31-47, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30470898

RESUMO

MAIN CONCLUSION: Hypercarotenogenesis in green algae evolved by mutation of PSY that increased its transcription at high light, disintegration of the eyespot in Dunaliella and acquisition of the capacity to export carotenoids from chloroplasts in Haematococcus. Carotenoids (Car) are lipid-soluble pigments synthesized in plants, algae, bacteria and fungi. Car have strong antioxidative properties and as such are utilized to reduce the danger of different diseases in humans. Two green microalgae are utilized as rich natural sources for Car: Dunaliella salina/bardawil accumulates 10% (w/w) ß-carotene (ßC), which is also pro-vitamin A, and Haematococcus pluvialis accumulates 4% (w/w) astaxanthin (Ast), the strongest antioxidant among Car. D. bardawil accumulates ßC in plastoglobules within the chloroplast, whereas H. pluvialis deposits Ast in cytoplasmic lipid droplets (CLD). In this review we compare the hypercarotenogenic responses (HCR) in Dunaliella and in Haematococcus and try to outline hypothetical evolutionary pathways for its origin. We propose that a mutation in phytoene synthetase that increased its transcription level in response to high light stress had a pivotal role in the evolution of the HCR. Proteomic analyses indicated that in D. bardawil/salina the HCR evolved from dissociation and amplification of eyespot lipid globules. The more robust HCR in algae that accumulate carotenoids in CLD, such as H. pluvialis, required also acquisition of the capacity to export ßC out of the chloroplast and its enzymatic conversion into Ast.


Assuntos
Carotenoides/metabolismo , Clorófitas/metabolismo , Gotículas Lipídicas/metabolismo , Geranil-Geranildifosfato Geranil-Geraniltransferase/metabolismo
3.
J Exp Bot ; 68(17): 4939-4950, 2017 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-28992231

RESUMO

The halotolerant green alga Dunaliella tertiolecta accumulates starch and triacylglycerol (TAG) amounting to 70% and 10-15% of total cellular carbon, respectively, when exposed to nitrogen (N) deprivation. The purpose of this study was to clarify the inter-relationships between the biosynthesis of TAG, starch, and polar lipids (PLs) in this alga. Pulse labeling with [14C]bicarbonate was utilized to label starch and [14C]palmitic acid (PlA) to label lipids. Transfer of 14C into TAG was measured and used to calculate rates of synthesis. About two-thirds of the carbon in TAG originates from starch, and one-third is made de novo by direct CO2 assimilation. The level made from degradation of pre-formed PLs is estimated to be very small. Most of the de novo synthesis involves fatty acid transfer through PLs made during the first day of N deprivation. The results suggest that starch made by photosynthetic carbon assimilation at the early stages of N deprivation is utilized for synthesis of TAG. Trans-acylation from PLs is the second major contributor to TAG biosynthesis. The utilization of starch for TAG biosynthesis may have biotechnological applications to optimize TAG biosynthesis in algae.


Assuntos
Radioisótopos de Carbono/metabolismo , Metabolismo dos Lipídeos , Amido/metabolismo , Triglicerídeos/biossíntese , Clorófitas
4.
Plant Cell Rep ; 36(6): 807-814, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28285407

RESUMO

KEY MESSAGE: We identified and demonstrated the function of 9-cis/all-trans ß-carotene isomerases in plastidic globules of Dunaliella bardawil, the species accumulating the highest levels of 9-cis ß-carotene that is essential for humans. The halotolerant alga Dunaliella bardawil is unique in that it accumulates under light stress high levels of ß-carotene in plastidic lipid globules. The pigment is composed of two major isomers: all-trans ß-carotene, the common natural form of this pigment, and 9-cis ß-carotene. The biosynthetic pathway of ß-carotene is known, but it is not clear how the 9-cis isomer is formed. We identified in plastidic lipid globules that were isolated from D. bardawil two proteins with high sequence homology to the D27 protein-a 9-cis/all-trans ß-carotene isomerase from rice (Alder et al. Science 335:1348-1351, 2012). The proteins are enriched in the oil globules by 6- to 17-fold compared to chloroplast proteins. The expression of the corresponding genes, 9-cis-ßC-iso1 and 9-cis-ßC-iso2, is enhanced under light stress. The synthetic proteins catalyze in vitro conversion of all-trans to 9-cis ß-carotene. Expression of the 9-cis-ßC-iso1 or of 9-cis-ßC-iso2 genes in an E. coli mutant line that harbors ß-carotene biosynthesis genes enhanced the conversion of all-trans into 9-cis ß-carotene. These results suggest that 9-cis-ßC-ISO1 and 9-cis-ßC-ISO2 proteins are responsible for the formation of 9-cis ß-carotene in D. bardawil under stress conditions.


Assuntos
Gotículas Lipídicas/metabolismo , beta Caroteno/metabolismo , Carotenoides/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Plantas/metabolismo , Plastídeos/metabolismo
5.
J Exp Bot ; 66(22): 7287-98, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26357883

RESUMO

In a recent study, it has been shown that biosynthesis of triacylglycerol (TAG) in the oleaginous green alga Chlorella desiccata is preceded by a large increase in acetyl-coenzyme A (Ac-CoA) levels and by upregulation of plastidic pyruvate dehydrogenase (ptPDH). It was proposed that the capacity to accumulate high TAG critically depends on enhanced production of Ac-CoA. In this study, two alternative Ac-CoA producers-plastidic Ac-CoA synthase (ptACS) and ATP citrate lyase (ACL)-are shown to be upregulated prior to TAG accumulation under nitrogen deprivation in the oleaginous species C. desiccata, but not in the moderate TAG accumulators Dunaliella tertiolecta and Chlamydomonas reinhardtii. Measurements of endogenous acetate production and of radiolabelled acetate incorporation into lipids are consistent with the upregulation of ptACS, but suggest that its contribution to the overall TAG biosynthesis is negligible. Induction of ACS and production of endogenous acetate are correlated with activation of alcohol dehydrogenase, suggesting that the upregulation of ptACS is associated with activation of PDH-bypass in C. desiccata. It is proposed that activation of the PDH-bypass in C. desiccata is needed to enable a high rate of lipid biosynthesis under nitrogen deprivation by controlling the level of pyruvate reaching ptPHD and/or mtPDH. This may be an important parameter for massive TAG accumulation in microalgae.


Assuntos
Acetato-CoA Ligase/metabolismo , Chlorella/enzimologia , Complexo Piruvato Desidrogenase/metabolismo , ATP Citrato (pro-S)-Liase/metabolismo , Álcool Desidrogenase/metabolismo , Ativação Enzimática , Nitrogênio/metabolismo , Oxigênio/metabolismo , Plastídeos/enzimologia , Triglicerídeos/metabolismo
6.
J Exp Bot ; 66(13): 3725-35, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25922486

RESUMO

Triglycerides (TAGs) from microalgae can be utilized as food supplements and for biodiesel production, but little is known about the regulation of their biosynthesis. This work aimed to test the relationship between acetyl-CoA (Ac-CoA) levels and TAG biosynthesis in green algae under nitrogen deprivation. A novel, highly sensitive liquid chromatography mass spectrometry (LC-MS/MS) technique enabled us to determine the levels of Ac-CoA, malonyl-CoA, and unacetylated (free) CoA in green microalgae. A comparative study of three algal species that differ in TAG accumulation levels shows that during N starvation, Ac-CoA levels rapidly rise, preceding TAG accumulation in all tested species. The levels of Ac-CoA in the high TAG accumulator Chlorella desiccata exceed the levels in the moderate TAG accumulators Dunaliella tertiolecta and Chlamydomonas reinhardtii. Similarly, malonyl-CoA and free CoA levels also increase, but to lower extents. Calculated cellular concentrations of Ac-CoA are far lower than reported K mAc-CoA values of plastidic Ac-CoA carboxylase (ptACCase) in plants. Transcript level analysis of plastidic pyruvate dehydrogenase (ptPDH), the major chloroplastic Ac-CoA producer, revealed rapid induction in parallel with Ac-CoA accumulation in C. desiccata, but not in D. tertiolecta or C. reinhardtii. It is proposed that the capacity to accumulate high TAG levels in green algae critically depends on their ability to divert carbon flow towards Ac-CoA. This requires elevation of the chloroplastic CoA pool level and enhancement of Ac-CoA biosynthesis. These conclusions may have important implications for future genetic manipulation to enhance TAG biosynthesis in green algae.


Assuntos
Acetilcoenzima A/biossíntese , Chlorella/metabolismo , Triglicerídeos/metabolismo , Acetil-CoA Carboxilase/metabolismo , Sequência de Aminoácidos , Chlorella/efeitos dos fármacos , Chlorella/crescimento & desenvolvimento , Coenzima A/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Malonil Coenzima A/metabolismo , Redes e Vias Metabólicas/efeitos dos fármacos , Dados de Sequência Molecular , Nitrogênio/farmacologia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Plastídeos/efeitos dos fármacos , Plastídeos/enzimologia , Piruvato Desidrogenase (Lipoamida)/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Padrões de Referência , Amido/metabolismo
7.
Plant Physiol ; 167(1): 60-79, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25404729

RESUMO

The halotolerant green alga Dunaliella bardawil is unique in that it accumulates under stress two types of lipid droplets: cytoplasmatic lipid droplets (CLD) and ß-carotene-rich (ßC) plastoglobuli. Recently, we isolated and analyzed the lipid and pigment compositions of these lipid droplets. Here, we describe their proteome analysis. A contamination filter and an enrichment filter were utilized to define core proteins. A proteome database of Dunaliella salina/D. bardawil was constructed to aid the identification of lipid droplet proteins. A total of 124 and 42 core proteins were identified in ßC-plastoglobuli and CLD, respectively, with only eight common proteins. Dunaliella spp. CLD resemble cytoplasmic droplets from Chlamydomonas reinhardtii and contain major lipid droplet-associated protein and enzymes involved in lipid and sterol metabolism. The ßC-plastoglobuli proteome resembles the C. reinhardtii eyespot and Arabidopsis (Arabidopsis thaliana) plastoglobule proteomes and contains carotene-globule-associated protein, plastid-lipid-associated protein-fibrillins, SOUL heme-binding proteins, phytyl ester synthases, ß-carotene biosynthesis enzymes, and proteins involved in membrane remodeling/lipid droplet biogenesis: VESICLE-INDUCING PLASTID PROTEIN1, synaptotagmin, and the eyespot assembly proteins EYE3 and SOUL3. Based on these and previous results, we propose models for the biogenesis of ßC-plastoglobuli and the biosynthesis of ß-carotene within ßC-plastoglobuli and hypothesize that ßC-plastoglobuli evolved from eyespot lipid droplets.


Assuntos
Proteínas de Algas/análise , Clorófitas/química , Citoplasma/química , Plastídeos/química , Proteoma/análise , beta Caroteno/análise , Proteínas de Algas/genética , Clorófitas/genética , Clorófitas/fisiologia , Lipídeos/análise , Filogenia , Análise de Sequência , beta Caroteno/biossíntese
8.
J Plant Physiol ; 171(12): 993-7, 2014 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-24974325

RESUMO

The mechanism of iron uptake in the chrysophyte microalga Dinobryon was studied. Previous studies have shown that iron is the dominant limiting elements for growth of Dinobryon in the Eshkol reservoir in northern Israel, which control its burst of bloom. It is demonstrated that Dinobryon has a light-stimulated ferrireductase activity, which is sensitive to the photosynthetic electron transport inhibitor DCMU and to the uncoupler CCCP. Iron uptake is also light-dependent, is inhibited by DCMU and by CCCP and also by the ferrous iron chelator BPDS. These results suggest that ferric iron reduction by ferrireductase is involved in iron uptake in Dinobryon and that photosynthesis provides the major reducing power to energize iron acquisition. Iron deprivation does not enhance but rather inhibits iron uptake contrary to observations in other algae.


Assuntos
Chrysophyta/metabolismo , Ferro/metabolismo , Microalgas/metabolismo , Chrysophyta/efeitos dos fármacos , Chrysophyta/crescimento & desenvolvimento , Chrysophyta/efeitos da radiação , Meios de Cultura/farmacologia , Inibidores Enzimáticos/farmacologia , FMN Redutase/antagonistas & inibidores , FMN Redutase/metabolismo , Ferro/farmacologia , Luz , Microalgas/efeitos dos fármacos , Microalgas/crescimento & desenvolvimento , Microalgas/efeitos da radiação , Fenantrolinas/farmacologia , Fotossíntese/efeitos dos fármacos , Fotossíntese/efeitos da radiação , Fatores de Tempo
9.
Plant Physiol ; 164(4): 2139-56, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24567188

RESUMO

The halotolerant microalgae Dunaliella bardawil accumulates under nitrogen deprivation two types of lipid droplets: plastoglobuli rich in ß-carotene (ßC-plastoglobuli) and cytoplasmatic lipid droplets (CLDs). We describe the isolation, composition, and origin of these lipid droplets. Plastoglobuli contain ß-carotene, phytoene, and galactolipids missing in CLDs. The two preparations contain different lipid-associated proteins: major lipid droplet protein in CLD and the Prorich carotene globule protein in ßC-plastoglobuli. The compositions of triglyceride (TAG) molecular species, total fatty acids, and sn-1+3 and sn-2 positions in the two lipid pools are similar, except for a small increase in palmitic acid in plastoglobuli, suggesting a common origin. The formation of CLD TAG precedes that of ßC-plastoglobuli, reaching a maximum after 48 h of nitrogen deprivation and then decreasing. Palmitic acid incorporation kinetics indicated that, at early stages of nitrogen deprivation, CLD TAG is synthesized mostly from newly formed fatty acids, whereas in ßC-plastoglobuli, a large part of TAG is produced from fatty acids of preformed membrane lipids. Electron microscopic analyses revealed that CLDs adhere to chloroplast envelope membranes concomitant with appearance of small ßC-plastoglobuli within the chloroplast. Based on these results, we propose that CLDs in D. bardawil are produced in the endoplasmatic reticulum, whereas ßC-plastoglobuli are made, in part, from hydrolysis of chloroplast membrane lipids and in part, by a continual transfer of TAG or fatty acids derived from CLD.


Assuntos
Clorófitas/metabolismo , Estruturas Citoplasmáticas/metabolismo , Lipídeos/química , beta Caroteno/metabolismo , Sequência de Aminoácidos , Western Blotting , Isótopos de Carbono , Clorofila/metabolismo , Clorófitas/genética , Clorófitas/ultraestrutura , Cloroplastos/metabolismo , Cloroplastos/ultraestrutura , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Estruturas Citoplasmáticas/ultraestrutura , Ácidos Graxos/metabolismo , Regulação da Expressão Gênica de Plantas , Processamento de Imagem Assistida por Computador , Dados de Sequência Molecular , Nitrogênio/deficiência , Oxazinas/metabolismo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Transporte Proteico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Fatores de Tempo , Tomografia , Triglicerídeos/metabolismo
10.
Planta ; 236(1): 19-33, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22231009

RESUMO

Many green algal species can accumulate large amounts of triacylglycerides (TAG) under nutrient deprivation, making them a potential source for production of biodiesel. TAG are organized in cytoplasmic lipid bodies, which contain a major lipid droplet protein termed MLDP. Green algae MLDP differ in sequence from plant oleosins and from animal perilipins, and their structure and function are not clear. In this study, we describe the isolation of MLDP from three species of the extreme halotolerant green algae Dunaliella. Sequence alignment with other green algae MLDP proteins identified a conserved 4-proline domain that may be considered as a signature domain of Volvocales green algae MLDP. Gold immunolabeling localized MLDP at the surface of lipid droplets in D. salina. The induction of MLDP by nitrogen deprivation is kinetically correlated with TAG accumulation, and inhibition of TAG biosynthesis impairs MLDP accumulation suggesting that MLDP induction is co-regulated with TAG accumulation. These results can lead to a better understanding of the structure and function of Volvocales green algae MLDP proteins.


Assuntos
Proteínas de Algas/biossíntese , Proteínas de Algas/isolamento & purificação , Clorófitas/química , Nitrogênio/deficiência , Plantas Tolerantes a Sal/química , Triglicerídeos/biossíntese , Triglicerídeos/isolamento & purificação , Sequência de Aminoácidos , Clorófitas/genética , Variação Genética , Genótipo , Metabolismo dos Lipídeos , Organelas/metabolismo , Plantas Tolerantes a Sal/genética , Alinhamento de Sequência
11.
J Phycol ; 48(5): 1209-19, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27011280

RESUMO

Astaxanthin-rich oil globules in Haematococcus pluvialis display rapid light-induced peripheral migration that is unique to this organism and serves to protect the photosynthetic system from excessive light. We observed rapid light-induced peripheral migration that is associated with chlorophyll fluorescence quenching, whereas the recovery was slow. A simple assay to follow globule migration, based on chlorophyll fluorescence level has been developed. Globule migration was induced by high intensity blue light, but not by high intensity red light. The electron transport inhibitor dichlorophenyl-dimethylurea did not inhibit globule migration, whereas the quinone analog (dibromo-methyl-isopropylbenzoquinone), induced globule migration even at low light. Actin microfilament-directed toxins, such as cytochalasin B and latrunculin A, inhibited the light-induced globule migration, whereas toxins against microtubules were ineffective. Electron microscopic (EM) imaging confirmed the cytoplasmic localization and peripheral migration of globules upon exposure to very high light (VHL). Scanning EM of freeze-fractured cells also revealed globules within cytoplasmic bridges traversing the chloroplast, presumably representing the pathway of migration. Close alignments of globules with endoplasmic reticulum (ER) membranes were also observed following VHL illumination. We propose that light-induced globule migration is regulated by the redox state of the photosynthetic electron transport system. Possible mechanisms of actin-based globule migration are discussed.

12.
J Microbiol Methods ; 88(2): 189-96, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22062087

RESUMO

Nile red (NR) is a popular fluorescent indicator to visualize lipid bodies in intact cells and has been extensively utilized to monitor triglyceride accumulation in microalgae. Typically, addition of NR to algae results in a rapid fluorescence enhancement followed by fluorescence quenching. NR fluorescence rise can be resolved into two kinetic phases: a fast phase (P1, sec), monitored at 525 nm/630 nm, followed by a slower phase (P2, min), monitored at 488 nm/575 nm. Studies with isolated plasma membrane (PM) and lipid globule (LG) preparations, suggest that P1 and P2 represent entry to the PM and transfer to LG, respectively. High NaCl slows down the interactions of NR with algae and with lipid globules. The onset of NR fluorescence quenching varies in different algae species between 5 min to 1h, and is observed in intact cells and in isolated LG. NR fluorescence quenching depends on NR concentration and is almost eliminated at low NR/cell ratios, indicating that it results from self-interactions of LG-associated dye. Glycerol has a dual effect on NR fluorescence: it eliminates kinetic anomalies resulting from limited solubility and self-interactions, but it also quenches NR fluorescence. NR fluorescence quenching by glycerol, as well as NR fluorescence enhancement by iodide anions, was observed only at high NR/LG ratios. These findings suggest that lipid-associated NR is more exposed to hydrophilic quenchers at high than at low NR concentrations. The results emphasize the importance of defining the optimal time window and NR concentrations for monitoring lipid accumulation in microalgae by NR fluorescence and clarify the origin of spectral anomalies resulting from self-interactions of dye molecules.


Assuntos
Corantes Fluorescentes/metabolismo , Microalgas/metabolismo , Oxazinas/química , Membrana Celular/química , Membrana Celular/metabolismo , Corantes Fluorescentes/química , Glicerol/química , Glicerol/metabolismo , Cinética , Lipossomos/química , Lipossomos/metabolismo , Microalgas/química , Oxazinas/metabolismo , Fosfatidilcolinas/química , Fosfatidilcolinas/metabolismo , Espectrometria de Fluorescência , Trioleína/química , Trioleína/metabolismo
13.
Lipids ; 46(9): 851-61, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21732215

RESUMO

Cytoplasmic oil globules of Haematococcus pluvialis (Chlorophyceae) were isolated and analyzed for pigments, lipids and proteins. Astaxanthin appeared to be the only pigment deposited in the globules. Triacyglycerols were the main lipids (more than 90% of total fatty acids) in both the cell-free extract and in the oil globules. Lipid profile analysis of the oil globules showed that relative to the cell-free extract, they were enriched with extraplastidial lipids. A fatty acids profile revealed that the major fatty acids in the isolated globules were oleic acid (18:1) and linoleic acid (18:2). Protein extracts from the globules revealed seven enriched protein bands, all of which were possible globule-associated proteins. A major 33-kDa globule protein was partially sequenced by MS/MS analysis, and degenerate DNA primers were prepared and utilized to clone its encoding gene from cDNA extracted from cells grown in a nitrogen depleted medium under high light. The sequence of this 275-amino acid protein, termed the Haematococcus Oil Globule Protein (HOGP), revealed partial homology with a Chlamydomonas reinhardtii oil globule protein and with undefined proteins from other green algae. The HOGP transcript was barely detectable in vegetative cells, but its level increased by more than 100 fold within 12 h of exposure to nitrogen depletion/high light conditions, which induced oil accumulation. HOGP is the first oil-globule-associated protein to be identified in H. pluvialis, and it is a member of a novel gene family that may be unique to green microalgae.


Assuntos
Clorófitas , Óleos de Plantas/isolamento & purificação , Proteínas de Plantas/isolamento & purificação , Sequência de Aminoácidos , Clorofila/metabolismo , Cromatografia Líquida de Alta Pressão , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Expressão Gênica , Interações Hidrofóbicas e Hidrofílicas , Dados de Sequência Molecular , Extratos Vegetais/química , Óleos de Plantas/química , Óleos de Plantas/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Alinhamento de Sequência , Xantofilas/química , Xantofilas/isolamento & purificação , Xantofilas/metabolismo
14.
Mol Cell Proteomics ; 6(9): 1459-72, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17569891

RESUMO

The halotolerant alga Dunaliella salina is a recognized model photosynthetic organism for studying plant adaptation to high salinity. The adaptation mechanisms involve major changes in the proteome composition associated with energy metabolism and carbon and iron acquisition. To clarify the molecular basis for the remarkable resistance to high salt, we performed a comprehensive proteomics analysis of the plasma membrane. Plasma membrane proteins were recognized by tagging intact cells with a membrane-impermeable biotin derivative. Proteins were resolved by two-dimensional blue native/SDS-PAGE and identified by nano-LC-MS/MS. Of 55 identified proteins, about 60% were integral membrane or membrane-associated proteins. We identified novel surface coat proteins, lipid-metabolizing enzymes, a new family of membrane proteins of unknown function, ion transporters, small GTP-binding proteins, and heat shock proteins. The abundance of 20 protein spots increased and that of two protein spots decreased under high salt. The major salt-regulated proteins were implicated in protein and membrane structure stabilization and within signal transduction pathways. The migration profiles of native protein complexes on blue native gels revealed oligomerization or co-migration of major surface-exposed proteins, which may indicate mechanisms of stabilization at high salinity.


Assuntos
Eucariotos/fisiologia , Proteômica/métodos , Sais/farmacologia , Proteínas de Algas/metabolismo , Sequência de Aminoácidos , Biotinilação , Membrana Celular/metabolismo , Cromatografia Líquida/métodos , Eletroforese em Gel Bidimensional , Eucariotos/metabolismo , Etiquetas de Sequências Expressas , Espectrometria de Massas/métodos , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Proteoma , Homologia de Sequência de Aminoácidos
15.
Plant Physiol ; 144(3): 1407-15, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17513481

RESUMO

Uptake of iron in the halotolerant alga Dunaliella salina is mediated by a transferrin-like protein (TTf), which binds and internalizes Fe(3+) ions. Recently, we found that iron deficiency induces a large enhancement of iron binding, which is associated with accumulation of three other plasma membrane proteins that associate with TTf. In this study, we characterized the kinetic properties of iron binding and internalization and identified the site of iron internalization. Iron deficiency induces a 4-fold increase in Fe binding, but only 50% enhancement in the rate of iron uptake and also increases the affinity for iron and bicarbonate, a coligand for iron binding. These results indicate that iron deprivation leads to accumulation and modification of iron-binding sites. Iron uptake in iron-sufficient cells is preceded by an apparent time lag, resulting from prebound iron, which can be eliminated by unloading iron-binding sites. Iron is tightly bound to surface-exposed sites and hardly exchanges with medium iron. All bound iron is subsequently internalized. Accumulation of iron inhibits further iron binding and internalization. The vacuolar inhibitor bafilomycin inhibits iron uptake and internalization. Internalized iron was localized by electron microscopy within vacuolar structures that were identified as acidic vacuoles. Iron internalization is accompanied by endocytosis of surface proteins into these acidic vacuoles. A novel kinetic mechanism for iron uptake is proposed, which includes two pools of bound/compartmentalized iron separated by a rate-limiting internalization stage. The major parameter that is modulated by iron deficiency is the iron-binding capacity. We propose that excessive iron binding in iron-deficient cells serves as a temporary reservoir for iron that is subsequently internalized. This mechanism is particularly suitable for organisms that are exposed to large fluctuations in iron availability.


Assuntos
Clorófitas/metabolismo , Ferro/metabolismo , Vacúolos/metabolismo , Cinética
16.
J Biol Chem ; 282(12): 8658-66, 2007 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-17227764

RESUMO

The halotolerant alga Dunaliella salina is unique among plants in that it utilizes a transferrin (TTf) to mediate iron acquisition (Fisher, M., Zamir, A., and Pick, U. (1998) J. Biol. Chem. 273, 17553-17558). Two new proteins that are induced by iron deprivation were identified in plasma membranes of D. salina as follows: a multicopper ferroxidase termed D-Fox and an internally duplicated glycoprotein (p130B). D-Fox and p130B are accessible to glycolytic, proteolytic, and biotin surface tagging treatments, suggesting that they are surface-exposed glycoproteins. Induction of D-Fox was also manifested by ferroxidase activity in plasma membrane preparations. These results are puzzling because ferroxidases in yeast and in Chlamydomonas reinhardtii function in redox-mediated iron uptake, a mechanism that is not known to operate in D. salina. Two lines of evidence suggest that D-Fox and p130B interact with D. salina triplicated transferrin (TTf). First, chemical cross-linking combined with mass spectroscopy analysis showed that D-Fox and p130B associate with TTf and with another plasma membrane transferrin. Second, detergent-solubilized D-Fox and p130B comigrated on blue native gels with plasma membrane transferrins. 59Fe autoradiography indicated that this complex binds Fe3+ ions. Also, the induction of D-Fox and p130B is kinetically correlated with enhanced iron binding and uptake activities. These results suggest that D-Fox and p130B associate with plasma membrane transferrins forming a complex that enhances iron binding and iron uptake. We propose that the function of D-Fox in D. salina has been modified during evolution from redox-mediated to transferrin-mediated iron uptake, following a gene transfer event of transferrins from an ancestral animal cell.


Assuntos
Proteínas de Algas/genética , Membrana Celular/metabolismo , Ceruloplasmina/química , Ceruloplasmina/genética , Eucariotos/metabolismo , Ferro/química , Transferrina/química , Proteínas de Algas/fisiologia , Sequência de Aminoácidos , Autorradiografia , Sequência de Bases , Ceruloplasmina/metabolismo , Ceruloplasmina/fisiologia , Clonagem Molecular , Reagentes de Ligações Cruzadas/farmacologia , Dados de Sequência Molecular , Oxirredução , Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase/metabolismo , Ligação Proteica , Homologia de Sequência de Aminoácidos
17.
J Exp Bot ; 57(14): 3583-94, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16968880

RESUMO

SPICK2, a homologue of the weakly-inward-rectifying Shaker-like Arabidopsis K channel, AKT2, is a candidate K+-influx channel participating in light- and clock-regulated leaf movements of the legume, Samanea saman. Light and the biological clock regulate the in situ K+-influx channel activity differentially in extensor and flexor halves of the pulvinus (the S. saman leaf motor organ), and also-though differently-the transcript level of SPICK2 in the pulvinus. This disparity between the in situ channel activity versus its candidate transcript, along with the sequence analysis of SPICK2, suggest an in situ regulation of the activity of SPICK2, possibly by phosphorylation and/or by interaction with cAMP. Consistent with this (i) the activity of the voltage-dependent K+-selective fraction of the inward current in extensor and flexor cells was affected differentially in whole-cell patch-clamp assays promoting phosphorylation (using the protein phosphatase inhibitor okadaic acid); (ii) several proteins in isolated plasma membrane-enriched vesicles of the motor cells underwent phosphorylation without an added kinase in conditions similar to patch-clamp; and (iii) the SPICK2 protein was phosphorylated in vitro by the catalytic subunit of the broad-range cAMP-dependent protein kinase. All of these results are consistent with the notion that SPICK2 is the K+-influx channel, and is regulated in vivo directly by phosphorylation.


Assuntos
Fabaceae/enzimologia , Proteínas de Plantas/metabolismo , Canais de Potássio Corretores do Fluxo de Internalização/metabolismo , Animais , Césio/farmacologia , AMP Cíclico/metabolismo , Condutividade Elétrica , Inibidores Enzimáticos/farmacologia , Fabaceae/citologia , Fabaceae/fisiologia , Insetos/genética , Proteínas de Membrana/metabolismo , Ácido Okadáico/farmacologia , Técnicas de Patch-Clamp , Fosforilação , Proteínas de Plantas/química , Canais de Potássio Corretores do Fluxo de Internalização/química , Estrutura Terciária de Proteína , RNA Mensageiro/metabolismo
18.
J Biol Chem ; 281(15): 10305-15, 2006 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-16469742

RESUMO

Adaptation of the halotolerant alga Dunaliella salina to iron deprivation involves extensive changes of chloroplast morphology, photosynthetic activities, and induction of a major 45-kDa chloroplast protein termed Tidi. Partial amino acid sequencing of proteolytic peptides suggested that Tidi resembles chlorophyll a/b-binding proteins which compose light-harvesting antenna complexes (LHC) (Varsano, T., Kaftan, D., and Pick, U. (2003) J. Plant Nutr. 26, 2197-2210). Here we show that Tidi shares the highest amino acid sequence similarity with light-harvesting I chlorophyll a/b-binding proteins from higher plants but has an extended proline-rich N-terminal domain. The accumulation of Tidi is reversed by iron supplementation, and its level is inversely correlated with photosystem I (PS-I) reaction center proteins. In native gel electrophoresis, Tidi co-migrates with enlarged PS-I-LHC-I super-complexes. Single particle electron microscopy analysis revealed that PS-I units from iron-deficient cells are larger (31 and 37 nm in diameter) than PS-I units from control cells (22 nm). The 77 K chlorophyll fluorescence emission spectra of isolated complexes suggest that the Tidi-LHC-I antenna are functionally coupled to the reaction centers of PS-I. These findings indicate that Tidi acts as an accessory antenna of PS-I. The enlargement of PS-I antenna in algae and in cyanobacteria under iron deprivation suggests a common limitation that requires rebalancing of the energy distribution between the two photosystems.


Assuntos
Clorofila/química , Eucariotos/metabolismo , Deficiências de Ferro , Complexo de Proteína do Fotossistema I/química , Sequência de Aminoácidos , Northern Blotting , Clorofila A , Clonagem Molecular , Cianobactérias/metabolismo , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Imuno-Histoquímica , Ferro/química , Ferro/metabolismo , Luz , Complexos de Proteínas Captadores de Luz/metabolismo , Microscopia Eletrônica , Dados de Sequência Molecular , Complexo de Proteína do Fotossistema I/metabolismo , Prolina/química , Estrutura Terciária de Proteína , Proteínas/química , RNA Mensageiro/metabolismo , Análise de Sequência de Proteína , Homologia de Sequência de Aminoácidos , Espectrometria de Fluorescência , Temperatura , Tilacoides/metabolismo , Fatores de Tempo , Transcrição Gênica
19.
Plant Physiol ; 136(1): 2806-17, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15333751

RESUMO

Salinity is a major limiting factor for the proliferation of plants and inhibits central metabolic activities such as photosynthesis. The halotolerant green alga Dunaliella can adapt to hypersaline environments and is considered a model photosynthetic organism for salinity tolerance. To clarify the molecular basis for salinity tolerance, a proteomic approach has been applied for identification of salt-induced proteins in Dunaliella. Seventy-six salt-induced proteins were selected from two-dimensional gel separations of different subcellular fractions and analyzed by mass spectrometry (MS). Application of nanoelectrospray mass spectrometry, combined with sequence-similarity database-searching algorithms, MS BLAST and MultiTag, enabled identification of 80% of the salt-induced proteins. Salinity stress up-regulated key enzymes in the Calvin cycle, starch mobilization, and redox energy production; regulatory factors in protein biosynthesis and degradation; and a homolog of a bacterial Na(+)-redox transporters. The results indicate that Dunaliella responds to high salinity by enhancement of photosynthetic CO(2) assimilation and by diversion of carbon and energy resources for synthesis of glycerol, the osmotic element in Dunaliella. The ability of Dunaliella to enhance photosynthetic activity at high salinity is remarkable because, in most plants and cyanobacteria, salt stress inhibits photosynthesis. The results demonstrated the power of MS BLAST searches for the identification of proteins in organisms whose genomes are not known and paved the way for dissecting molecular mechanisms of salinity tolerance in algae and higher plants.


Assuntos
Clorófitas/genética , Clorófitas/metabolismo , Proteínas de Algas/biossíntese , Proteínas de Algas/genética , Proteínas de Algas/isolamento & purificação , Clorófitas/efeitos dos fármacos , Eletroforese em Gel Bidimensional , Metabolismo Energético , Espectrometria de Massas , Modelos Biológicos , Oxirredução , Fotossíntese , Proteômica , Cloreto de Sódio/farmacologia
20.
Biometals ; 17(1): 79-86, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-14977364

RESUMO

It is demonstrated that Antimycin A (AA), a respiratory inhibitor produced by Streptomyces bacteria, forms lipophylic complexes with Fe(III) ions. Spectroscopic titration indicates that Fe(III) ions interact with 2AA molecules. At growth-limiting Fe concentrations, AA mediates Fe uptake and promotes growth and chlorophyll synthesis better than other Fe chelators in the halotolerant alga Dunaliella salina. It is proposed that AA enhances Fe bioavailability in hypersaline solutions by formation of lipophylic Fe-AA complexes which are taken-up and utilized by the algae. The results suggest that the respiratory inhibitor AA can affect Fe metabolism in microorganisms.


Assuntos
Antimicina A/metabolismo , Antimicina A/farmacologia , Clorófitas/efeitos dos fármacos , Clorófitas/metabolismo , Ferro/metabolismo , Respiração/efeitos dos fármacos , Antimicina A/química , Bicarbonatos/farmacologia , Cátions/antagonistas & inibidores , Cátions/química , Cátions/metabolismo , Quelantes/farmacologia , Clorofila/metabolismo , Ácido Cítrico/química , Ácido Edético/farmacologia , Ferro/antagonistas & inibidores , Ferro/química , Ligantes , Lipossomos/química , Estrutura Molecular , Análise Espectral , Titulometria
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