Concentration of the main persistent organic pollutants in sewage sludge in relation to wastewater treatment plant parameters and sludge stabilisation.

Concentration of 16 polycyclic aromatic hydrocarbons (PAHs), 7 polychlorinated biphenyls (PCBs), and 11 organochlorine pesticides (OCPs) in sewage sludge from 40 wastewater treatment plants (WWTPs) was investigated. Relationship between pollutant sludge contents, main WWTP parameters and type of sludge stabilisation was carefully evaluated. Average load of PAHs, PCBs, and OCPs in different sludges from Czech Republic was 3096, 95.7 and 76.1 µg/kg dry weight, respectively. There were moderate/strong correlations among the individual tested pollutants in sludge (r = 0.40-0.76). Relationship between total pollutant contents in sludge, common WWTP parameters and sludge stabilisation was not evident. Only individual pollutants such anthracene and PCB 52 correlated significantly (P < 0.05) with biochemical oxygen demand (r = -0.35) and chemical oxygen demand removal efficiencies (r = -0.35), suggesting recalcitrance to degradation during wastewater treatment. When sorted according to the design capacity, a linear correlation between WWTP size and pollutant contents in sludge was evident with growing WWTP capacity. Our study indicated that WWTPs with anaerobic digestion are prone to accumulate a statistically higher content of PAHs and PCBs (P < 0.05) in digested sludges compared to aerobically digested ones. The influence of anaerobic digestion temperature of treated sludge on tested pollutants was not evident.

Biochemical and physiological changes in Zea mays L. after exposure to the environmental pharmaceutical pollutant carbamazepine.

The presence of pharmaceuticals in the environment is a matter of great concern. They are consistently found in the environment, raising concerns regarding human exposure through dietary intake. In this study, we observed the effect of the application of carbamazepine at 0.1, 1, 10, and 1000 µg per kg of soil contamination levels to assess stress metabolism in Zea mays L. cv. Ronaldinio at the 4th leaf, tasselling, and dent phenological stages. The transfer of carbamazepine to the aboveground and root biomass was assessed, and uptake increased dose-dependently. No direct effect on biomass production was observed, but multiple physiological and chemical changes were observed. Major effects were consistently observed at the 4th leaf phenological stage for all contamination levels, including reduced photosynthetic rate, reduced maximal and potential activity of photosystem II, decreased water potential, decreased carbohydrates (glucose and fructose) and γ-aminobutyric acid in roots, and increased maleic acid and phenylpropanoids (chlorogenic acid and its isomer, 5-O-caffeoylquinic acid) in aboveground biomass. A reduction in net photosynthesis was observed for the older phenological stages, whereas no other relevant and consistent physiological and metabolic changes related to contamination exposure were detected. Our results indicate that Z. mays can overcome the environmental stress caused by the accumulation of carbamazepine with notable metabolic changes at the early phenological stage; however, older plants adapted and only exhibited minor effects in the presence of the contaminant. The potential implications for agricultural practice could be associated with the plant's response to simultaneous stresses due to metabolite changes associated with oxidative stress.

Tomato (Solanum lycopersicum L.) accumulation and allergenicity in response to nickel stress.

Vegetables represent a major source of Ni exposure. Environmental contamination and cultural practices can increase Ni amount in tomato posing significant risk for human health. This work assesses the tomato (Solanum lycopersicum L.) response to Ni on the agronomic yield of fruits and the related production of allergens. Two cultivars were grown in pots amended with Ni 0, 30, 60, 120, and 300 mg kg-1, respectively. XRF and ICP-MS analyses highlighted the direct increase of fruit Ni content compared to soil Ni, maintaining a stable biomass. Leaf water content increased at Ni 300 mg kg-1. Total protein content and individual allergenic components were investigated using biochemical (RP-HPLC and N-terminal amino acid sequencing) and immunological (inhibition tests of IgE binding by SPHIAa assay on the FABER testing system) methodologies. Ni affected the fruit tissue concentration of pathogenesis-related proteins and relevant allergens (LTP, profilin, Bet v 1-like protein and TLP). This study elucidates for the first time that tomato reacts to exogenous Ni, uptaking the metal while changing its allergenic profiles, with potential double increasing of exposure risks for consumers. This evidence highlighted the importance of adequate choice of low-Ni tomato cultivars and practices to reduce Ni uptake by potentially contaminated matrices.

Occurrence of synthetic polycyclic and nitro musk compounds in sewage sludge from municipal wastewater treatment plants.

Synthetic musk compounds (SMCs) are widely used as fragrances that can be released from different sources and through the sewer system, finally reaching wastewater treatment plants (WWTPs). In this study, 6 synthetic polycyclic and 5 nitro musk compounds were screened in 55 sewage sludge (SS) samples from 43 different WWTPs in the Czech Republic, and the effect of WWTP technology parameters on SMC content in SS was assessed. Galaxolide and Tonalide were predominant synthetic polycyclic musk compounds (SPMCs) detected in all SS tested and accounted for 99.5% of the average content of sludge SMCs (5518 µg/kg dw). The amount of synthetic nitro musk compounds (SNMCs) in SS samples was negligible. The Tonalide content in SS correlated significantly with the WWTP design capacity (r = 0.32, P < 0.05). The significant correlation between chemical oxygen demand (COD) removal efficiency and SMCs (r = -0.37, P < 0.05) partly suggests the recalcitrance of SMCs, mainly of Celestolide, Galaxolide and Tonalide, to biodegradation in WWTPs. A statistically lower SNMC content was found in anaerobically digested sludges than in aerobic ones. There was no significant difference (P > 0.05) between the digestion technology as well as the temperature of anaerobic digestion on the SPMC content in sewage sludge. The wastewater (WW) load percentage or WW hydraulic retention time had no influence on the SMC content in the resulting SS. Musk compounds did not change over time when the SS samples were analysed with a gap of two years, suggesting that sewage sludge for soil applications only needs to be analysed for musk compounds once a year. Our study indicates that the currently common WWTP technologies have only very limited potential to affect the accumulation of musk compounds in sewage sludge.