Biocompatibility, Surface Morphology, and Bacterial Load of Dental Implant Abutments following Decontamination Protocols: An In-Vitro Study.

The long-term success of dental implant rehabilitation depends significantly on proper peri-implant soft tissue integration. Therefore, decontamination of abutments prior to their connection to the implant is beneficial to enhance soft tissue attachment and to aid in maintaining marginal bone around the implant. Consequently, different implant abutment decontamination protocols were evaluated regarding biocompatibility, surface morphology, and bacterial load. The protocols evaluated were autoclave sterilization, ultrasonic washing, steam cleaning, chlorhexidine chemical decontamination, and sodium hypochlorite chemical decontamination. The control groups included: (1) implant abutments prepared and polished in a dental lab without decontamination and (2) unprepared implant abutments obtained directly from the company. Surface analysis was performed using scanning electron microscopy (SEM). Biocompatibility was evaluated using XTT cell viability and proliferation assays. Biofilm biomass and viable counts (CFU/mL) (n = 5 for each test) were used for surface bacterial load evaluation. Surface analysis revealed areas of debris and accumulation of materials, such as iron, cobalt, chromium, and other metals, in all abutments prepared by the lab and with all decontamination protocols. Steam cleaning was the most efficient method for reducing contamination. Chlorhexidine and sodium hypochlorite left residual materials on the abutments. XTT results showed that the chlorhexidine group (M = 0.7005, SD = 0.2995) had the lowest values (p < 0.001) (autoclave: M = 3.6354, SD = 0.1510; ultrasonic: M = 3.4077, SD = 0.3730; steam: M = 3.2903, SD = 0.2172; NaOCl: M = 3.5377, SD = 0.0927; prep non-decont.: M = 3.4815, SD = 0.2326; factory: M = 3.6173, SD = 0.0392). Bacterial growth (CFU/mL) was high in the abutments treated with steam cleaning and ultrasonic bath: 2.93 × 109, SD = 1.68 × 1012 and 1.83 × 109, SD = 3.95 × 1010, respectively. Abutments treated with chlorhexidine showed higher toxicity to cells, while all other samples showed similar effects to the control. In conclusion, steam cleaning seemed to be the most efficient method for reducing debris and metallic contamination. Bacterial load can be reduced using autoclaving, chlorhexidine, and NaOCl.

Comparison of Different Finishing and Polishing Systems on Surface Roughness and Bacterial Adhesion of Resin Composite.

Insufficient dental restoration finishing and polishing may lead to plaque accumulation, gingival inflammation, staining, caries, and esthetic impairment. Here, the effect of two finishing and polishing systems on surface roughness and bacterial adhesion were evaluated. Two finishing and polishing kits were evaluated: diamond burs (Shine 1-2, Strauss & Co, Raanana, Israel) and paper discs (Sof-Lex 3M ESPE, Seefeld, Germany) (n = 30 each). For each group surface roughness was evaluated using an optical profilometer (Contour GT-K1, Bruker, Billerica, MA, USA) (n = 10). Surface bacteria were evaluated for biofilm biomass using crystal violet (CV) staining (absorbance measured at 538 nm) and viable counts (CFU/mL) (n = 20). The control group included polymerized discs against a Mylar strip (n = 30). Student's t test and one-way ANOVA were used for statistical evaluation. Diamond burs, paper discs, and control average surface RA were 169.4 ± 45.2 µ, 364 ± 77.7 µ, and 121.2 ± 18.1 µ, respectively. There was a significant difference found between all groups (p < 0.00001). Bacterial biomass on diamond burs, paper discs, and control samples were 0.458 ± 0.161, 0.507 ± 0.139, and 0.446 ± 0.142, respectively (p = 0.257). Viable bacterial counts (CFU/mL) on diamond burs, paper discs, and control samples were 2.25 × 104, 2.95 × 104, and 2.75 × 104, respectively (p = 0.856). A comparison between two finishing and polishing kits showed that the shine 1−2 diamond bur kit produced a smoother surface than the polishing disc kit. No differences were found in the biofilm biomass quantification and bacterial viable count between the groups.

Correlation Between the Tragus-Incisal Distance and Interocclusal Rest Distance.

PURPOSE: To evaluate correlations of arch size and sex with the interocclusal rest distance (IORD), as well as to estimate proportional variance. MATERIALS AND METHODS: A total of 106 participants were examined. The participants, 38 men and 68 women, were aged 22 to 30 years, were fully dentate, had no signs of abnormal abrasion, and had intact posterior occlusal contacts. Measurements of interocclusal rest distance and tragus-incisal distance were recorded, and the rest angle created between the tragus-incisal distance in maximum intercuspation and in resting vertical dimension were calculated according to the cosine formula. Correlation between the size of the mandible (tragus-incisal distance, mean of left and right sides) and the IORD were calculated using Pearson correlation coefficient. Correlations for sex (calculated separately for male and female) and rest angle were also assessed. RESULTS: The mean (SD) tragus-incisal distance values were 123.38 (6.77) mm for all participants, 120.01 (4.64) mm for women, and 130.72 (5.24) mm for men. The mean (SD) IORD values were 2.76 (1.3) mm for all participants, 2.13 (0.9) mm for women, and 3.87 (1.17) mm for men. The mean (SD) rest angle values were 1.26 (0.55) degrees for all participants, 1.02 (0.41) degrees for women, and 1.7 (0.49) degrees for men. Pearson correlation coefficient between IORD and tragus-incisal distance was significant (P < .05). According to t test, there was a significant difference between men and women for IORD, tragus-incisal distance, and rest angle (P < .01). CONCLUSION: A correlation exists between IORD and arch size. A statistically significant difference was found between men and women for IORD and arch size values.

Determining the interchangeability of KaVo PROTAR semi-adjustable articulators.

OBJECTIVE: Interchangeable articulators are crucial for a precise clinical outcome. Lack of interchangeability will affect the accuracy of the occlusion of prosthetic restorations. The objective of this study was to evaluate the interchangeability of KaVo PROTARevo articulators. METHOD AND MATERIALS: KaVo PROTARevo semi-adjustable articulators in routine use were employed. Identical casts were transferred among the articulators and the distance between casts was measured using digital photos of sagittal and frontal aspects of each articulator by two independent examiners. Interchangeability was assessed and analyzed by designated software. RESULTS: Inter-examiner reliability was found to be high. Of 231 possible pairs of articulators only 27 pairs were found to be interchangeable (less than 166 µm in all measured dimensions), and of the other 204 pairs of articulators at least one of the dimensions measured was larger than 166 µm. CONCLUSION: The majority of articulators tested did not pass the 166-µm criterion to establish them as interchangeable. Therefore, in order to maintain accuracy while producing restorations, it is advisable to use the same articulator throughout the entire process, both at the clinic and in the dental laboratory.

Antibacterial Activity of Orthodontic Cement Containing Quaternary Ammonium Polyethylenimine Nanoparticles Adjacent to Orthodontic Brackets.

Enamel demineralization is a common problem found in patients using orthodontic devices, such as orthodontic braces. It was found that Streptoccocus mutans growth increases adjacent to orthodontic devices, which may result in caries development. Incorporated antibacterial quaternary ammonium polyethylenimine (QPEI) nanoparticles were previously shown to be highly efficacious against various bacteria. Combining antibacterial materials in orthodontic cement may be advantageous to prevent bacterial outgrowth adjacent to orthodontic brackets. The aim was to evaluate the efficiency of orthodontic cement containing QPEI nanoparticles in reducing S. mutans and Lactobacillus casei outgrowth adjacent to orthodontic brackets. Orthodontic brackets were bonded to the buccal surfaces of extracted lower incisors. The antibacterial effect on S. mutans and L. casei outgrowth of Neobond bracket adhesive orthodontic cement with and without QPEI nanoparticles was compared. The antibacterial effect was evaluated using crystal violet staining and bacterial count (CFU/mL). The teeth in the experimental group, with the QPEI nanoparticles cement, showed significantly lower optical density (OD) values and CFU counts of S. mutans and L. casei than the teeth in the control group (p < 0.05). Based on the results, it can be concluded that orthodontic cement containing QPEI nanoparticles significantly inhibits S. mutans and L. casei growth around orthodontic brackets.

Antibacterial effect of composite resin foundation material incorporating quaternary ammonium polyethyleneimine nanoparticles.

STATEMENT OF PROBLEM: As caries is the most frequent cause of the failure of composite resin-based restorations, composite resins with antibacterial properties are desirable. However, whether quaternary ammonium polyethyleneimine nanoparticles can be effectively incorporated is unknown. PURPOSE: The purpose of this in vitro study was to evaluate the antibacterial activity against Streptococcus mutans and Actinomyces viscosus of a foundation material incorporating quaternary ammonium polyethyleneimine (QPEI) nanoparticles. MATERIAL AND METHODS: QPEI antimicrobial nanoparticles were incorporated in a commercially available foundation material (Q Core; BJM Laboratories Ltd) at 1% wt/wt. Antibacterial efficacy against S mutans (106 colony-forming units [CFU]/mL) and A viscosus (106 CFU/mL) was examined by the direct contact test (DCT), and the agar diffusion test (ADT) with and without surface polishing. Bacterial outgrowth was recorded with a spectrophotometer. RESULTS: Growth of S mutans and A viscosus was inhibited, showing a decrease by 6 orders of magnitude in bacterial viability in specimens incorporating the nanoparticles, even after polishing the foundation material (P<.05). Growth inhibition was not observed in specimens without nanoparticles. CONCLUSIONS: Antibacterial properties can be achieved in a commercially available foundation material by incorporating polycationic antibacterial nanoparticles. This antibacterial effect did not diminish after surface polishing.