Aerobic biodegradation of bio-based plastics in the seawater/sediment interface (sublittoral) marine environment of the coastal zone - Test method under controlled laboratory conditions.

Pollution of the seas due to plastic litter is a rapidly growing environmental problem. Among several actions, legal and technological, undertaken to alleviate this problem, included are the control of single use conventional plastics and the replacement of conventional non-biodegradable plastics with innovative biodegradable-in-the-sea polymers, both aiming at the mitigation of marine litter accumulation. Laboratory tests have been used to characterize plastics regarding their biodegradation in various environments. Biodegradation of plastics depends on the inherent characteristics of the polymer and the particular marine habitat conditions. In the present work, the international standard test method ISO 19679 (2016) for determining the aerobic biodegradation of non-floating plastic materials in a seawater-sediment interface of the coastal marine zone under laboratory simulated conditions is evaluated. Modifications are proposed to improve the reliability of this test method in some aspects. Agitation of the seawater surface in the bioreactor was found to enhance the continuous availability of oxygen at the seawater-sediment interface, thus assuring aerobic biodegradation conditions throughout the test simulating real sublittoral conditions. Additional recommended improvements include the use of larger samples and supplementary nutrients to optimise biodegradation conditions. The laboratory measurements were validated by field experiments. The proposed modifications and refinements optimise the robustness and reliability of the test method for determining aerobic biodegradation of plastic materials in a simulated seawater-sediment interface of the coastal zone.

Disintegration behaviour of bio-based plastics in coastal zone marine environments: A field experiment under natural conditions.

The accumulation of plastic wastes in the marine environment represents a steadily increasing global environmental threat. The replacement of conventional plastics with bio-based biodegradable materials may contribute to alleviating the problem in the long run. This work studies the disintegration behaviour of three bio-based plastic materials, namely Polyhydroxybutyrate (PHB), Polybutylene sebacate (PBSe), Polybutylene sebacate-co-terephthalate (PBSeT), in three different coastal zone marine environments under natural conditions. The three studied environments were: 1) the seashore zone which is periodically covered by the seawater due to waves or tide, called eulittoral or intertidal zone; 2) the water column zone of small depth (about 10 m), called pelagic zone; and 3) the interface zone between the water column and the seabed sediment at small depth (about 20 m), called sublittoral or benthic zone. The experiments took place in the Aegean Sea at the SW coast of Salamis Island. The results showed that disintegration, as an indicative measure of biodegradation, occurs in all three tested environments, even though the rate depends on the material, the habitat, and the prevailing during the testing period environmental conditions. The degrees of disintegration of all materials in the three environments exhibited significant differences: Benthic > Intertidal > Pelagic. The observed disintegration can be attributed to biodegradation since the negative reference Low-density Polyethylene (LLDPE) material did not disintegrate.