RESUMO
A panel of markers, including MAbs to different epitopes of CEA, B 6.2, detection of AP activity and lectin receptors (PNA, HPL, SBA, LAL, LcL) for cell identification in pleural effusions, is proposed. Using immunocytochemical methods cancer cells were determined in all 80 cytological positive and in 13 from 21 cytologically negative cancer effusions. The reaction was not observed in 45 benign effusions. The panel was unsuccessful to determine the tumor cell origin. The immunophenotypic features of reactive transformed lymphocytes in effusions were described and the criteria for diagnosis of B-cell and T-cell NHL were present. By means of these criteria lymphomatous cells in serous effusions of seven patients were revealed.
Assuntos
Anticorpos Monoclonais , Biomarcadores Tumorais/análise , Lectinas , Derrame Pleural/patologia , Antígeno Carcinoembrionário/análise , Feminino , Humanos , Imuno-Histoquímica , Queratinas/análise , Neoplasias/patologia , Receptores Mitogênicos/análiseRESUMO
Data are presented on Ehrlich ascitic tumor cells energetic metabolism, activities of the glycolytic enzymes and the pentose phosphate pathway enzymes, contents and synthesis rates of the macromolecules at various cell cycle stages. An attempt was made to correct the direct measurement by taking into consideration a systematic error introduced in the experiment by incomplete cells synchronization. Cell metabolism activation sharply increased at two mitotic cycle stages. At the first stage (end of G1-period, beginning of S-period) the processes associated with the preparation to reduplication and DNA synthesis were activated. The second activation wave (end of S-period, G2-period) was connected with cell preparation of mitosis. The coordination of cell metabolism variations during the cell cycle is shown.
Assuntos
Carcinoma de Ehrlich/metabolismo , Leucemia L1210/metabolismo , Leucemia P388/metabolismo , Animais , Transporte Biológico Ativo , Ciclo Celular , Colesterol/metabolismo , Metabolismo Energético , Interfase , Malato Desidrogenase/metabolismo , Mitose , Ácidos Neuramínicos/metabolismo , Ácidos Nucleicos/biossíntese , Via de Pentose Fosfato , Biossíntese de Proteínas , Fase S , Timidina/metabolismoRESUMO
Spontaneous and induced chemiluminescence of rat blood plasma following irradiation of the animals with fast neurons was studied. Dynamics of the luminescence reflected the degree of radiation injury and an oscillatory response of blood chemiluminescent effect was observed.
Assuntos
Sangue/efeitos da radiação , Medições Luminescentes , Animais , Nêutrons Rápidos , Radicais Livres , Cinética , Masculino , Oxigênio/sangue , Lesões Experimentais por Radiação/sangue , Lesões Experimentais por Radiação/diagnóstico , RatosRESUMO
Monoclonal antibodies (mAbs) IPO-3 and IPO-10 were generated following immunization of a BALB/c mice with human cell lines RPMI-1788 and Daudi respectively. The reactivity of these mAbs was studied by indirect immunofluorescence technique with 10 human cell lines, blood cells of healthy persons and patients with the malignant lymphoproliferative diseases. Studies of normal and neoplastic B cells suggest that mAbs IPO-3 and IPO-10 which recognize antigens are B lineage restricted. The presence of an antigen defined by the mAb IPO-10 appears to include most but not all the stages of B cell differentiation, whereas IPO-3 detected antigen is not represented on resting B cells and has a very limited expression on activated B lymphocytes. The results obtained with mAbs IPO-3 and IPO-10 are discussed in relation to other known B cell surface markers.
