Thirty years' achievements in prevention and control of hepatitis B virus infection / 中国实用内科杂志

Hepatitis B vaccination and blocking mother-to-child transmission of HBV are of great significance in the prevention and control of HBV infection. Hepatitis B vaccination is the most effective way to prevent HBV infection. Mother-tochild transmission of HBV can be greatly reduced by these measures, which include strengthening the screening of HBV in women of childbearing age, antiviral treatment in pregnant women with high viral load, and combined immunization of hepatitis B vaccine and hepatitis B immunoglobulin for newborns of HBsAg positive mothers. In the recent 30 years, remarkable achievements have been made in the prevention and control of HBV infection in China.

Human bone marrow-derived mesenchymal stem cells differentiate into epidermal-like cells in vitro.

Human bone marrow-derived mesenchymal stem cells (hMSCs) are a population of pluripotent cells. They can differentiate into different embryonic layer cells as osteoblasts, adipocytes, chondrocytes, myoblasts, neurocytes, etc. However, there are only few reports with regard to differentiate hMSCs into epidermal cells in vitro. In this study, we want to investigate the feasibility of inducing hMSCs into epidermal-like cells under specific medium in vitro. hMSCs in specific inducing medium expressed the early markers of epidermal cell lineage, P63, cytokeratin19 (CK19), the late differentiated marker, the pan-cytokeratin, and another early marker, the beta1-integrin, which up-regulated remarkably in inducing medium. Their morphologies were changed from spindle-like fibroblastic appearances to oblate or irregular shapes under phase contrast microscopy. The hemidesmosome structure was found using the transmission electron microscope. All these data suggested that, under certain conditions, hMSCs have the potential to differentiate into epidermal-like cells. It will be of great accordance in the study of the multipotential property of hMSCs.

Study on differentiation of human mesenchymal stem cells into epidermal cells / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the possibility of differentiation of human mesenchymal stem cells (hMSC) into epidemic cells in vitro.</p><p><b>METHODS</b>hMSCs were segregated from normal adult human bone marrow by Percoll solution (1.073 g/ml) , and were cultured, purified, and amplified to 3th passage in vitro. Then the hMSCs were randomly divided into control group ( with treatment of normal L-DMEM medium) and experimental group (with treatment of L-DMEM medium containing epidermal growth factor,insulin,tretinoin, calcium chloride). After 7 days of culture, the morphologic changes of hMSCs in the 2 groups were observed with inverted phase contrast microscope. The expressions of P63 and PCK of hMSCs were assessed with immunohistochemical methods.</p><p><b>RESULTS</b>The shape of hMSCs in experimental group became irregular or oblong in shape, while that in control group were still in spindle shape. Immunohistochemical results showed that hMSCs were P63 and PCK positive in the experimental group, while those in control group were negative.</p><p><b>CONCLUSION</b>Human mesenchymal stem cells can differentiate into epidemic cell in vitro.</p>

Isolation, culturation and adipogenisis committed differentiation of adult human mesenchymal stem cell / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To isolate MSCs from adult human bone marrow cells and to induce them into adipocytes.</p><p><b>METHODS</b>MSCs were isolated from adult human bone marrow aspirated by Percoll and expanded in L-DMEM. The surface antigen of MSCs, CD14, CD34, CD45, CD44, VLA-1, HLA-DR and cell cycle were analysed on a FACScan flow cytometer. MSCs were cultured in adipogenisis inducing medium including insulin, 1-methyl-3-isobutylxanthine, indomethine and dexamethasone for 7 days and stained with Oil Red O.</p><p><b>RESULTS</b>MSCs grew as adherent cells and expanded more than 10 passages. They were positive for CD44 and negative for CD14, CD34, CD45, HLA-DR. The expression of VLA-1 was weak. After 7 days of adipocyte inducing, about 85%of the cells displayed accumulation of lipid vacuoles, as detected by Red Oil O.</p><p><b>CONCLUSION</b>MSCs isolated and cultured from adult human bone marrow can be induced to adipogenisis committed differentiation.</p>