1.
Chemphyschem
; 13(11): 2649-53, 2012 Aug 06.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22707114
RESUMO
A universal label-free method for the spectroscopic investigation of polyhistidine-tagged proteins is presented. A solid supported lipid bilayer (SSLB, picture) containing nitrilotriacetic-acid-modified lipids is attached on top of a germanium attenuated total reflection crystal by hydrophilic interactions. Any His tag-modified protein can be immobilized and investigated by FTIR spectroscopy.