RESUMO
Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) in vitro. Vitis labrusca L. (variety Concord) were produced organically and by a conventional system, and their juice was extracted by a hot extraction process. The organic grapes were subjected to ultraviolet-type C radiation (UV-C). Experiments were performed after production and after 6 months in storage. Physicochemical analyses revealed that UV-C irradiation of organic grapes, the juice production process, and storage resulted in nutraceutical alterations. However, none of the juice concentrations were cytotoxic to HTC cells by the cytokinesis-blocked proliferation index results or were mutagenic, because the formation of micronucleated cells was not induced. In general, juice induced cell proliferation, possibly due to the presence of vitamins and sugar content (total soluble solid). The data increased the understanding of food technology and confirmed the quality and safety consumption of these juices.
Assuntos
Proliferação de Células , Dano ao DNA , Manipulação de Alimentos/normas , Armazenamento de Alimentos/normas , Sucos de Frutas e Vegetais/normas , Vitis/química , Animais , Carboidratos/análise , Linhagem Celular Tumoral , Manipulação de Alimentos/métodos , Qualidade dos Alimentos , Armazenamento de Alimentos/métodos , Sucos de Frutas e Vegetais/análise , Sucos de Frutas e Vegetais/efeitos da radiação , Ratos , Raios Ultravioleta , Vitaminas/análiseRESUMO
Os autores apresentam um caso de fistula pieloduodenal de origem traumatica discutindo suas etiologias e tratamento
Assuntos
Adulto , Humanos , Masculino , Duodenopatias , Fístula , Pelve Renal , Ferimentos por Arma de FogoRESUMO
Os autores apresentam sua experiência com a cirurgia de Boari para a correçäo das lesöes do ureter terminal, fazendo incialmente uma análise das principais possibilidades de correçäo destas lesöes. Mostrando os bons resultados que obtiveram em quadro casos operados pela técnica de Boari, discutindo as vantagens e as desvantagens da técnica, terminam por concluir que se trata de uma boa soluçäo para a correçäo das lesöes do ureter terminal, principalmente nos casos operados mais tardiamente em que se encontra grande fibrose no local
