Chasing Flies: The Use of Wingbeat Frequency as a Communication Cue in Calyptrate Flies (Diptera: Calyptratae).

The incidental sound produced by the oscillation of insect wings during flight provides an opportunity for species identification. Calyptrate flies include some of the fastest and most agile flying insects, capable of rapid changes in direction and the fast pursuit of conspecifics. This flight pattern makes the continuous and close recording of their wingbeat frequency difficult and limited to confined specimens. Advances in sound editor and analysis software, however, have made it possible to isolate low amplitude sounds using noise reduction and pitch detection algorithms. To explore differences in wingbeat frequency between genera and sex, 40 specimens of three-day old Sarcophaga crassipalpis, Lucilia sericata, Calliphora dubia, and Musca vetustissima were individually recorded in free flight in a temperature-controlled room. Results showed significant differences in wingbeat frequency between the four species and intersexual differences for each species. Discriminant analysis classifying the three carrion flies resulted in 77.5% classified correctly overall, with the correct classification of 82.5% of S. crassipalpis, 60% of C. dubia, and 90% of L. sericata, when both mean wingbeat frequency and sex were included. Intersexual differences were further demonstrated by male flies showing significantly higher variability than females in three of the species. These observed intergeneric and intersexual differences in wingbeat frequency start the discussion on the use of the metric as a communication signal by this taxon. The success of the methodology demonstrated differences at the genus level and encourages the recording of additional species and the use of wingbeat frequency as an identification tool for these flies.

Domestic Filth Flies in New Haven, Connecticut: A Case Study on the Effects of Urbanization and Climate Change by Comparing Fly Populations after 78 Years.

Changes in common and widespread insect populations such as the domestic filth fly in urban cities are useful and relevant bioindicators for overall changes in the insect biomass. The current study surveyed necrophagous flies by placing a weekly trap from June-September over a two-year period in the city of New Haven, Connecticut, to compare data on fly abundance and diversity with data collected 78 years earlier. Climate and land cover changes were also assessed in combination with the fly population for each period. The survey results suggest the domestic filth fly population is now less diverse with decreased species richness and changes in the relative abundance of species. In both surveys, 95-96% of the population was composed of only three species. The current survey data indicate the numerical dominance of Lucilia sericata has decreased, the abundance of several species, notably Lucilia coeruleiviridis, has increased, and Lucilia illustris is absent. Species that showed a significant interaction with temperature in the 1940s survey have now increased in abundance, with several of the trapped species continuing to show an interaction with temperature and rainfall. Analysis of the land cover and climate data characterizes the trap site as a region exposed to a prolonged period of industrialization and urbanization, with only 7% of the land cover remaining undeveloped and over 50% impervious, coupled with an increase in temperature and rainfall. This study serves as a model for changes in domestic filth fly populations and other insects in similarly highly urbanized established cities.

Forensically relevant blow flies (Diptera: Calliphoridae) of Central Connecticut, USA.

Connecticut and the Northeastern United States in general, lacks forensically relevant entomological survey data. To determine which forensically relevant calliphorid (blow flies, Diptera: Calliphoridae) and non-calliphorid species dominate Connecticut, 5 traps using pork bait were set out 7 times over a 2-year period to collect adult and immature specimens. Insects collected from human corpses in this region were also tabulated to collaborate the forensic relevance of trapped specimens. The survey identified a total of 8 species of blow flies of the Lucilia and Calliphora genera, 6 of which colonized the baits, and 5 of which have been found to colonize human corpses in the area. Non-calliphorid genera Sarcophaga and Muscina were also found to be colonizers of the baited traps but in relatively lower numbers. Trap sites differed significantly in the degree of urbanization which was determined by using GIS mapping to classify a 1 kilometer (km) radius around each site using land use and the percent of urban impervious surfaces. The 1 km radius revealed the highly fragmented nature of the immediate habitats of the trap sites and no habitat or seasonal preference was demonstrated by blow flies under these trap conditions. Temperature was the one variable which significantly affected the number of flies trapped and the colonizing species. All trapped species have been described previously as widespread and common in the United States and as synanthropic. Further research in this region should trap at the constantly changing extreme ends of the urban-rural gradient and in the colder temperatures of winter to explore the limits of the flexibility of these blow fly species.