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1.
Curr Top Med Chem ; 19(14): 1241-1251, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31223088

RESUMO

BACKGROUND: Schistosomiasis is a neglected disease, which affects millions of people in developing countries. Its treatment relies on a single therapeutic alternative, the praziquantel. This situation may lead to drug resistance which, in turn, made urgent the need for new antischistosomal agents. Nacylhydrazones are usually explored as good antimicrobial agents, but the vanillin-related N-acylhydrazones have never been tested by their antiparasitic potential. OBJECTIVE: Herein, we report the synthesis of seven analogues, three of them unpublished, their biological investigation against Schistosoma mansoni and Target Fishing studies. METHODS: The compounds were synthesized following classical synthetical approaches. The anthelmintic potential was assessed as well as their cytotoxicity profile. Confocal laser scanning microscopy and target fishing study were performed to better understand the observed antischistosomal activity. RESULTS: Compound GPQF-407 exhibited good antischistosomal activity (47.91 µM) with suitable selectivity index (4.14). Confocal laser scanning microscopy revealed that it triggered severe tegumental destruction and tubercle disintegration. Target fishing studies pointed out some probable targets, such as the serine-threonine kinases, dihydroorotate dehydrogenases and carbonic anhydrase II. CONCLUSION: The GPQF-407 was revealed to be a promising antischistosomal agent which, besides presenting the N-acylhydrazone privileged scaffold, also could be easily synthesized on large scales from commercially available materials.


Assuntos
Benzaldeídos/farmacologia , Hidrazonas/farmacologia , Schistosoma mansoni/efeitos dos fármacos , Esquistossomicidas/farmacologia , Animais , Benzaldeídos/química , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Relação Dose-Resposta a Droga , Hidrazonas/síntese química , Hidrazonas/química , Estrutura Molecular , Esquistossomicidas/síntese química , Esquistossomicidas/química , Relação Estrutura-Atividade , Células Vero
2.
Artigo em Inglês | MEDLINE | ID: mdl-30559137

RESUMO

Schistosomiasis is a parasitic flatworm disease that infects over 200 million people worldwide, especially in poor communities. Treatment and control of the disease rely on just one drug, praziquantel. Since funding for drug development for poverty-associated diseases is very limited, drug repurposing is a promising strategy. In this study, from a screening of 13 marketed diuretics, we identified that spironolactone, a potassium-sparing diuretic, had potent antischistosomal effects on Schistosoma mansoniin vitro and in vivo in a murine model of schistosomiasis. In vitro, spironolactone at low concentrations (<10 µM) is able to alter worm motor activity and the morphology of adult schistosomes, leading to parasitic death. In vivo, oral treatment with spironolactone at a single dose (400 mg/kg) or daily for five consecutive days (100 mg/kg/day) in mice harboring either patent or prepatent infections significantly reduced worm burden, egg production, and hepato- and splenomegaly (P < 0.05 to P < 0.001). Taken together, with the safety profile of spironolactone, supported by its potential to affect schistosomes, these results indicate that spironolactone could be a potential treatment for schistosomiasis and make it promising for repurposing.


Assuntos
Reposicionamento de Medicamentos/métodos , Schistosoma mansoni/efeitos dos fármacos , Esquistossomose/tratamento farmacológico , Esquistossomicidas/farmacologia , Espironolactona/farmacologia , Animais , Modelos Animais de Doenças , Diuréticos/farmacologia , Feminino , Masculino , Camundongos , Praziquantel/farmacologia
3.
Chem Biodivers ; 15(12): e1800398, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30276965

RESUMO

In this study, we evaluated the in vitro and in vivo schistosomicidal activities of chalcones against Schistosoma mansoni worms. In vitro assays revealed that chalcones 1 and 3 were the most active compounds, without affecting significantly mammalian cells. Confocal laser scanning microscopy and scanning electron microscopy studies revealed reduction on the numbers of tubercles and morphological alterations in the tegument of S. mansoni worms after in vitro incubation with chalcones 1 and 3. In a mouse model of schistosomiasis, the oral treatment (400 mg/kg) with chalcone 1 or 3 significantly caused a total worm burden reduction in mice. Chalcone 1 showed significant inhibition of the S. mansoni ATP diphosphohydrolase activity, which was corroborated by molecular docking studies. The results suggested that chalcones could be explored as lead compounds with antischistosomal properties.


Assuntos
Anti-Helmínticos/química , Chalconas/farmacologia , Schistosoma mansoni/efeitos dos fármacos , Administração Oral , Animais , Anti-Helmínticos/síntese química , Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Apirase/antagonistas & inibidores , Apirase/metabolismo , Sítios de Ligação , Chalconas/síntese química , Chalconas/química , Chalconas/uso terapêutico , Modelos Animais de Doenças , Proteínas de Helminto/antagonistas & inibidores , Proteínas de Helminto/metabolismo , Camundongos , Microscopia Confocal , Microscopia Eletrônica de Varredura , Simulação de Acoplamento Molecular , Estrutura Terciária de Proteína , Schistosoma mansoni/enzimologia , Esquistossomose mansoni/tratamento farmacológico , Esquistossomose mansoni/patologia , Relação Estrutura-Atividade
4.
Toxicol In Vitro ; 50: 1-10, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29476885

RESUMO

Schistosomiasis, caused by helminth flatworms of the genus Schistosoma, is one of the most important parasitic diseases in the world, affecting over 200 million people in developing countries. Riparins are natural alkamides found in Aniba riparia (Lauraceae) fruits that possess several pharmacological properties. In this study, we reported the synthesis, characterization and structural analysis of six riparin derivatives (A-F), as well as their schistosomicidal activity against S. mansoni worms together with a biological, pharmacokinetic and toxicological in silico evaluation. Firstly, these compounds were synthesized, purified and characterized by elemental analysis, FT-IR spectroscopy, X-ray diffraction and theoretical calculations to evaluate their stability and conformation. Next, the schistosomicidal activity of the riparins was tested against S. mansoni worms. Bioassays revealed that Riparins E and F were the most active compounds, showing half-maximum inhibitory concentration at low micromolar ranges (IC50 values ~10 µM). Also, confocal laser scanning microscopy studies revealed tegumental damage in parasites after exposition with Riparins B, E and F. Additionally, based on MTT assay, all tested riparins showed no cytotoxic potential toward mammalian cells. Finally, in silico analyses were used to predict the absorption, distribution, metabolism, elimination and toxicity (ADMET) of the compounds. Taken together, the results revealed a promising ADMET profile and suggested that riparins could be starting points for lead optimization programs for natural products with antischistosomal properties.


Assuntos
Benzamidas , Fenetilaminas , Esquistossomicidas , Animais , Benzamidas/química , Benzamidas/farmacocinética , Benzamidas/farmacologia , Benzamidas/toxicidade , Células CACO-2 , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Simulação por Computador , Humanos , Absorção Intestinal , Modelos Biológicos , Estrutura Molecular , Fenetilaminas/química , Fenetilaminas/farmacocinética , Fenetilaminas/farmacologia , Fenetilaminas/toxicidade , Difração de Pó , Schistosoma mansoni/efeitos dos fármacos , Esquistossomicidas/química , Esquistossomicidas/farmacocinética , Esquistossomicidas/farmacologia , Esquistossomicidas/toxicidade , Absorção Cutânea , Espectroscopia de Infravermelho com Transformada de Fourier , Células Vero , Difração de Raios X
5.
Toxicol In Vitro ; 44: 273-279, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28755871

RESUMO

Oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. In this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis­1-benzyl-N-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans­N-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of Bacillus cereus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Serratia marcescens, Shigella flexneri and Staphylococcus aureus by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Additionally, the ex vivo antiparasitic activity of oxazines was assessed against Schistosoma mansoni, a helminth that is one of the major agents of the disease schistosomiasis Also, oxazines were evaluated on three tumor cell lines, NCI-H292 (human lung carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (human cervix carcinoma), and two normal cell lines (Vero and red blood cells). Bioassays revealed that oxazine 2 is more effective against bacteria than oxazine 1, with the lowest MIC and MBC values of 3.91 and 32.5µg/mL, respectively. Similarly, compound 2 demonstrated higher antiparasitic activity than 1, and scanning electron microscopy analysis showed several morphological alterations in the tegument of worms in a concentration-dependent manner. In contrast, both oxazines exhibited low cytotoxic effects on cancer and normal cell lines. These results indicated that oxazines exerted direct effects on bacteria and parasite schistosomes. More importantly, since schistosomiasis control programs rely on one drug, praziquantel, oxazines may have the potential to become new antischistosomal agents.


Assuntos
Antibacterianos/farmacologia , Cicloexenos/farmacologia , Oxazinas/farmacologia , Esquistossomicidas/farmacologia , Animais , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Schistosoma mansoni/efeitos dos fármacos , Schistosoma mansoni/ultraestrutura , Ovinos
6.
Int J Antimicrob Agents ; 50(3): 467-472, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28666754

RESUMO

Schistosomiasis is a major public health problem worldwide, especially in poor communities. Since praziquantel is currently the only drug available to treat schistosomiasis, there is an urgent need to identify new antischistosomal drugs. Nerolidol is a sesquiterpene present as an essential oil in several plants that has been approved by the FDA. This study evaluated the in vivo antischistosomal activity of nerolidol in a mouse model of schistosomiasis infected with either adult or juvenile stages of Schistosoma mansoni. A single dose of nerolidol (100, 200 or 400 mg/kg) administered orally to mice infected with adult schistosomes resulted in a reduction in worm burden and egg production. Treatment with the highest nerolidol dose (400 mg/kg) caused significant reduction in a total worm burden of 70.06% (P < 0.001). Additionally, the technique of quantitative and qualitative oograms showed that a single 400 mg/kg nerolidol dose achieved an immature egg reduction of 84.6% (P < 0.001). In faecal samples, the Kato-Katz method also revealed a reduction of 75.2% in eggs/g at a dose of 400 mg/kg (P < 0.001). Furthermore, scanning electron microscopy revealed that nerolidol-mediated worm killing was associated with tegumental damage. In contrast to activity against adult S. mansoni infection, oral treatment with nerolidol 400 mg/kg had low efficacy in mice harbouring juvenile schistosomes. Since nerolidol is already in use globally as a food additive and has a proven safety record, evaluation of this natural compound's potential for treatment of schistosomiasis could be entirely cost effective in the near future.


Assuntos
Anti-Helmínticos/administração & dosagem , Anti-Helmínticos/farmacologia , Schistosoma mansoni/efeitos dos fármacos , Esquistossomose mansoni/tratamento farmacológico , Sesquiterpenos/administração & dosagem , Sesquiterpenos/farmacologia , Administração Oral , Animais , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Fezes/parasitologia , Feminino , Masculino , Camundongos Endogâmicos BALB C , Microscopia Eletrônica de Varredura , Contagem de Ovos de Parasitas , Carga Parasitária , Schistosoma mansoni/ultraestrutura , Resultado do Tratamento
7.
Phytother Res ; 31(4): 624-630, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28111828

RESUMO

Pilocarpus microphyllus Stapf ex Wardlew (Rutaceae), popularly known as jaborandi, is a plant native to the northern and northeastern macroregions of Brazil. Several alkaloids from this species have been isolated. There are few reports of antibacterial and anthelmintic activities for these compounds. In this work, we report the antibacterial and anthelmintic activity of five alkaloids found in P. microphyllus leaves, namely, pilosine, epiisopilosine, isopilosine, epiisopiloturine and macaubine. Of these, only anthelmintic activity of one of the compounds has been previously reported. Nuclear magnetic resonance, HPLC and mass spectrometry were combined and used to identify and confirm the structure of the five compounds. As regards the anthelmintic activity, the alkaloids were studied using in vitro assays to evaluate survival time and damaged teguments for Schistosoma mansoni adult worms. We found epiisopilosine to have anthelmintic activity at very low concentrations (3.125 µg mL-1 ); at this concentration, it prevented mating, oviposition, reducing motor activity and altered the tegument of these worms. In contrast, none of the alkaloids showed antibacterial activity. Additionally, alkaloids displayed no cytotoxic effect on vero cells. The potent anthelmintic activity of epiisopilosine indicates the potential of this natural compound as an antiparasitic agent. Copyright © 2017 John Wiley & Sons, Ltd.


Assuntos
Alcaloides/química , Anti-Helmínticos/química , Antibacterianos/química , Imidazóis/química , Pilocarpus/química , Extratos Vegetais/química , Folhas de Planta/química , 4-Butirolactona/análogos & derivados , Animais , Imidazóis/farmacologia , Células Vero
8.
Chem Biol Interact ; 244: 129-39, 2016 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-26697994

RESUMO

The use of natural products has a long tradition in medicine, and they have proven to be an important source of lead compounds in the development of new drugs. Among the natural compounds, terpenoids present broad-spectrum activity against infective agents such as viruses, bacteria, fungi, protozoan and helminth parasites. In this study, we report a biological screening of 38 chemically characterized terpenes from different classes, which have a hydroxyl group connected by hydrophobic chain or an acceptor site, against the blood fluke Schistosoma mansoni, the parasite responsible for schistosomiasis mansoni. In vitro bioassays revealed that 3,7-dimethyl-1-octanol (dihydrocitronellol) (10) was the most active terpene (IC50 values of 13-52 µM) and, thus, we investigated its antischistosomal activity in greater detail. Confocal laser scanning microscopy revealed that compound 10 induced severe tegumental damage in adult schistosomes and a correlation between viability and tegumental changes was observed. Furthermore, we compared all the inactive compounds with dihydrocitronellol structurally by using shape and charge modeling. Lipophilicity (miLogP) and other molecular properties (e.g. molecular polar surface area, molecular electrostatic potential) were also calculated. From the 38 terpenes studied, compound 10 is the one with the greatest flexibility, with a sufficient apolar region by which it may interact in a hydrophobic active site. In conclusion, the integration of biological and chemical analysis indicates the potential of the terpene dihydrocitronellol as an antiparasitic agent.


Assuntos
Anti-Helmínticos/química , Anti-Helmínticos/farmacologia , Schistosoma mansoni/efeitos dos fármacos , Terpenos/química , Terpenos/farmacologia , Animais , Cricetinae , Relação Dose-Resposta a Droga , Estrutura Molecular , Testes de Sensibilidade Parasitária , Relação Estrutura-Atividade
9.
Phytomedicine ; 22(10): 921-8, 2015 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-26321741

RESUMO

BACKGROUND: Schistosomiasis is one of the world's major public health problems, and praziquantel (PZQ) is the only available drug to treat this neglected disease with an urgent demand for new drugs. Recent studies indicated that extracts from Piper aduncum L. (Piperaceae) are active against adult worms of Schistosoma mansoni, the major etiological agent of human schistosomiasis. PURPOSE: We investigated the in vitro schistosomicidal activity of cardamonin, a chalcone isolated from the crude extract of P. aduncum. Also, this present work describes, for the first time, the S. mansoni ATP diphosphohydrolase inhibitory activity of cardamonin, as well as, its molecular docking with S. mansoni ATPDase1, in order to investigate its mode of inhibition. METHODS: In vitro schistosomicidal assays and confocal laser scanning microscopy were used to evaluate the effects of cardamonin on adult schistosomes. Cell viability was measured by MTT assay, and the S. mansoni ATPase activity was determined spectrophotometrically. Identification of the cardamonin binding site and its interactions on S. mansoni ATPDase1 were made by molecular docking experiments. RESULTS: A bioguided fractionation of the crude extract of P. aduncum was carried out, leading to identification of cardamonin as the active compound, along with pinocembrin and uvangoletin. Cardamonin (25, 50, and 100 µM) caused 100% mortality, tegumental alterations, and reduction of oviposition and motor activity of all adult worms of S. mansoni, without affecting mammalian cells. Confocal laser scanning microscopy showed tegumental morphological alterations and changes on the numbers of tubercles of S. mansoni worms in a dose-dependent manner. Cardamonin also inhibited S. mansoni ATP diphosphohydrolase (IC50 of 23.54 µM). Molecular docking studies revealed that cardamonin interacts with the Nucleotide-Binding of SmATPDase 1. The nature of SmATPDase 1-cardamonin interactions is mainly hydrophobic and hydrogen bonding. CONCLUSION: This report provides evidence for the in vitro schistosomicidal activity of cardamonin and demonstrated, for the first time, that this chalcone is highly effective in inhibiting S. mansoni ATP diphosphohydrolase, opening the route to further studies of chalcones as prototypes for new S. mansoni ATP diphosphohydrolase inhibitors.


Assuntos
Apirase/antagonistas & inibidores , Chalconas/farmacologia , Piper/química , Schistosoma mansoni/efeitos dos fármacos , Esquistossomicidas/farmacologia , Animais , Chlorocebus aethiops , Inibidores Enzimáticos/farmacologia , Feminino , Masculino , Simulação de Acoplamento Molecular , Estrutura Molecular , Schistosoma mansoni/enzimologia , Células Vero
10.
Antimicrob Agents Chemother ; 59(10): 6648-52, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26239976

RESUMO

In recent years, a class of oxindole-copper and -zinc complex derivatives have been reported as compounds with efficient proapoptotic activity toward different tumor cells (e.g., neuroblastomas, melanomas, monocytes). Here we assessed the efficacy of synthesized oxindole-copper(II), -zinc(II), and -vanadyl (VO(2+)) complexes against adult Schistosoma mansoni worms. The copper(II) complexes (50% inhibitory concentrations of 30 to 45 µM) demonstrated greater antischistosomal properties than the analogous zinc and vanadyl complexes regarding lethality, reduction of motor activity, and oviposition.


Assuntos
Anti-Helmínticos/química , Anti-Helmínticos/farmacologia , Complexos de Coordenação/farmacologia , Schistosoma mansoni/efeitos dos fármacos , Animais , Complexos de Coordenação/química , Cobre/química , Zinco/química
11.
PLoS Negl Trop Dis ; 9(3): e0003656, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25816129

RESUMO

Schistosomiasis is a serious disease currently estimated to affect more that 207 million people worldwide. Due to the intensive use of praziquantel, there is increasing concern about the development of drug-resistant strains. Therefore, it is necessary to search for and investigate new potential schistosomicidal compounds. This work reports the in vivo effect of the alkaloid epiisopiloturine (EPI) against adults and juvenile worms of Schistosoma mansoni. EPI was first purified its thermal behavior and theoretical solubility parameters charaterised. In the experiment, mice were treated with EPI over the 21 days post-infection with the doses of 40 and 200 mg/kg, and 45 days post-infection with single doses of 40, 100 and 300 mg/kg. The treatment with EPI at 40 mg/kg was more effective in adult worms when compared with doses of 100 and 300 mg/kg. The treatment with 40 mg/kg in adult worms reduced parasite burden significantly, lead to reduction in hepatosplenomegaly, reduced the egg burden in faeces, and decreased granuloma diameter. Scanning electron microscopy revealed morphological changes to the parasite tegument after treatment, including the loss of important features. Additionally, the in vivo treatment against juvenile with 40 mg/kg showed a reduction of the total worm burden of 50.2%. Histopathological studies were performed on liver, spleen, lung, kidney and brain and EPI was shown to have a DL50 of 8000 mg/kg. Therefore EPI shows potential to be used in schistosomiasis treatment. This is the first time that schistosomicidal in vivo activity of EPI has been reported.


Assuntos
4-Butirolactona/análogos & derivados , Imidazóis/farmacologia , Schistosoma mansoni/efeitos dos fármacos , Esquistossomose mansoni/tratamento farmacológico , Esquistossomicidas/farmacologia , 4-Butirolactona/farmacologia , Animais , Relação Dose-Resposta a Droga , Fezes/parasitologia , Granuloma/patologia , Fígado/efeitos dos fármacos , Fígado/parasitologia , Camundongos , Microscopia Eletrônica de Varredura , Schistosoma mansoni/ultraestrutura
13.
Molecules ; 19(3): 3793-803, 2014 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-24662089

RESUMO

Schistosomiasis is a neglected tropical disease that affects hundreds of millions of people worldwide. Since the treatment of this disease currently relies on a single drug, praziquantel, new and safe schistosomicidal agents are urgently required. Nerolidol, a sesquiterpene present in the essential oils of several plants, is found in many foods and was approved by the U.S. Food and Drug Administration. In this study we analysed the in vitro antiparasitic effect of nerolidol on Schistosoma mansoni adult worms. Nerolidol at concentrations of 31.2 and 62.5 µM reduced the worm motor activity and caused the death of all male and female schistosomes, respectively. In addition, confocal laser scanning microscopy revealed morphological alterations on the tegument of worms such as disintegration, sloughing and erosion of the surface, and a correlation between viability and tegumental damage was observed. In conclusion, nerolidol may be a promising lead compound for the development of antischistosomal natural agents.


Assuntos
Esquistossomicidas/química , Esquistossomicidas/farmacologia , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Terpenos/química , Animais , Microscopia Confocal , Testes de Sensibilidade Parasitária , Schistosoma mansoni/efeitos dos fármacos
14.
PLoS Negl Trop Dis ; 8(1): e2617, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24392173

RESUMO

BACKGROUND: Schistosomiasis is a major endemic disease that affects hundreds of millions worldwide. Since the treatment and control of this parasitic disease rely on a single drug, praziquantel, it is imperative that new effective drugs are developed. Here, we report that phytol, a diterpene alcohol from chlorophyll widely used as a food additive and in medicinal fields, possesses promising antischistosomal properties in vitro and in a mouse model of schistosomiasis mansoni. METHODS AND FINDINGS: In vitro, phytol reduced the motor activity of worms, caused their death and confocal laser scanning microscopy analysis showed extensive tegumental alterations in a concentration-dependent manner (50 to 100 µg/mL). Additionally, phytol at sublethal doses (25 µg/mL) reduced the number of Schistosoma mansoni eggs. In vivo, a single dose of phytol (40 mg/kg) administered orally to mice infected with adult S. mansoni resulted in total and female worm burden reductions of 51.2% and 70.3%, respectively. Moreover, phytol reduced the number of eggs in faeces (76.6%) and the frequency of immature eggs (oogram pattern) was significantly reduced. The oogram also showed increases in the proportion of dead eggs. Confocal microcopy studies revealed tegumental damage in adult S. mansoni recovered from mice, especially in female worms. CONCLUSIONS: The significant reduction in parasite burden by this chlorophyll molecule validates phytol as a promising drug and offers the potential of a new direction for chemotherapy of human schistosomiasis. Phytol is a common food additive and nonmutagenic, with satisfactory safety. Thus, phytol has potential as a safe and cost-effective addition to antischistosomal therapy.


Assuntos
Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Fitol/farmacologia , Fitol/uso terapêutico , Schistosoma mansoni/efeitos dos fármacos , Esquistossomose mansoni/tratamento farmacológico , Administração Oral , Animais , Modelos Animais de Doenças , Feminino , Locomoção/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Carga Parasitária , Testes de Sensibilidade Parasitária , Análise de Sobrevida
15.
Rev. Inst. Adolfo Lutz ; 63(2): 243-247, jul.-dez. 2004. graf
Artigo em Português | LILACS, Sec. Est. Saúde SP | ID: lil-404807

RESUMO

Este trabalho objetivou levantar as parasitoses intestinais mais freqüentes na faixa etária de 6 a 11 anos, relacionando-as ao nível sócio-econômico e aos hábitos de vida das crianças de uma escola pública de primeiro grau no município de Catanduva. Iniciou-se com palestras sobre educação sanitária para pais e alunos. Posteriormente, foi aplicado um questionário sócio-econômico e colheram-se amostras de fezes de 250 crianças, sendo 138 do sexo masculino e 112 do sexo feminino. Foram feitos exames parasitológicos de fezes; as crianças, que apresentaram resultados positivos, foram tratadas. Das crianças analisadas, 12,80% estavam parasitadas, principalmente aquelas na faixa etária de 8 a 9 anos (40,62%) e 62,50% eram do sexo masculino. As 84,37% de crianças parasitadas das crianças parasitadas e 67,88% das crianças não parasitadas relataram que tinham animais de estimação. Os parasitas mais encontrados foram Giardia lamblia (43,75%); Entamoeba coli (31,25%); Enterobius vermicularis (18,75%); Endolimax nana (15,62%); ancilostomídeos (3,12%); Strongyloides stercoralis (3,12%) e Entamoeba hystolitica (3,12%).Das crianças positivas, 81,25% eram monoparasitadas e 18,7% poliparasitadas ; e 77,27% bebiam água diretamente da torneira. Concluiu-se que a incidência de parasitoses diminuiu no município de Catanduva se comparada a dos anos anteriores, mostrando ter havido melhoria na qualidade de vida e maior conhecimento das pessoas em relação à educação sanitária e saneamento básico


Assuntos
Humanos , Masculino , Feminino , Criança , Doenças Parasitárias , Educação em Saúde , Estudantes , Saneamento Básico
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