RESUMO
Preliminary purification of alpha 2-interferon from the culture liquid on cation-exchange sorbents was studied. Application of a cellosorbent based on the cationite KU-23 resulted in a 20-25-fold purification of recombinant alpha 2-interferon in a one-step process as a result of combination of the sieve effect and ion-exchange chromatography.
Assuntos
Interferon-alfa/isolamento & purificação , Celulose , Cromatografia em Gel , Cromatografia por Troca Iônica , Interferon alfa-2 , Proteínas RecombinantesRESUMO
The interaction of microdispersions of crosslinked polyelectrolytes with ions of biologically active substances has been studied by physicochemical methods. The processes of ion exchange and physical adsorption, underlying the interaction, can promote flocculation. Of particular importance is the selective flocculation of dipolar ions of biologically active substances.
Assuntos
Fatores Biológicos , Biotecnologia , Eletrólitos , Reagentes de Ligações Cruzadas , Concentração de Íons de Hidrogênio , TermodinâmicaRESUMO
The effect of heat treatment used in the course of tuberculin production by Mycobacteria tuberculosis cells on the structure and biological activity of tuberculin was studied. Methods of preparative and analytical gel-filtration were used. The largest quantity of the biologically active component was obtained after 2-hour heat treatment of the mycobacteria culture.