Study of antimutagenic and anticytotoxic effects of biorag in case of mutations induced by ammonium nitrate.

The antimutagenic and anticytotoxic effects of bioactivator - biorag (created by Prof. R. Gakhokidze) in the case of mutations induced by ammonium nitrate (NH(4)NO(3)) were studied on the laboratory mice. The cytogenetic and toxicological methods of investigation were used in our research. Ammonium nitrate is characterized by mutagenic, cytotoxic and consequently general toxic action. Introduction of ammonium nitrate (doze 1/2, 1/5 LD(50)) per oral to animals induces strong increase (p<0,001) of frequency of chromosomal aberrations (multiple fragmentation, lyses), a genomic mutations (triploidy, tetraploidy), pathological mitosis (K-mitosis, hollow metaphase, adhesion of chromosomes) and destruction of interphase nucleuses (hollow nucleus). Biorag is characterized with greatly expressed antimutagenic and anticytotoxic effect and statistically reliable reduces mutagenic and cytotoxic effect of ammonium nitrate. At separate effect of ammonium nitrate (dose 1/2 LD(50)) the frequency of chromosomal anomalies was 8,8%, pathologic mitosis - 21,4%, interphase nucleus destruction - 4,5%. After addition of biorag in diet, these indexes decreases accordingly to 3,0%; 8,6% and 1,5%. (p<0,001). On the base of conducted experiments application of biorag for medical purpose is prospective, especially for people who are in contact with harmful, mutagenic substances, also for the individuals poisoned with pesticides and fertilizers.

[The influence of polyvitamin complex polijen on mutagenic and cytotoxic effect of copper oxychloride in white mice].

The present study investigates antimutagenic and anticytotoxic effect of polyvitamin complex Polijen in laboratory mice. Mutation in mice was induced by pesticide - copper oxychloride 3Cu(OH)2 CuCl2 , which is characterizes by the mutagenic and cytotoxic effect. Introduction of copper oxychloride (dosage 1/2, 1/5, 1/10 LD50) per oral to animals induces strong increase (p<0,001) of frequency of chromosomal aberrations (multiple fragments, lyses), genomic mutations (triploidy, tetraploidy), pathological mitosis (hollow metaphase, K-mitosis, adhesion of chromosomes) and destruction of interphase nucleuses (hollow nucleus). Experiments showed that administration of polyvitamin complex Polijen decreased 2, 5 times mutagenic and cytotoxic effect of pesticide-copper oxychloride.

[Effect of endogenous interferon on the embryo-lethal and mutagenic action of the influenza virus in white mice]. / Vliianie éndogennogo interferona na émbrioletal'noe i mutagennoe deístvie virusa grippa u belykh myshei.

The influence of endogenous interferon on chromosomes of pregnant mice and their fetuses in influenza infection as well as embryolethal effect of influenza virus under protection of interferon induction were studied. Infection of pregnant mice with influenza A2/Aichi virus produced significant chromosomal aberrations in mothers and their fetuses.