RESUMO
OBJECTIVES: During holmium:yttrium-aluminum-garnet (holmium:YAG) laser lithotripsy to break urinary stones, urologists frequently see flashes of light. As infrared laser pulses are invisible, what is the source of light? Here we studied the origin, characteristics, and some effects of flashes of light in laser lithotripsy. METHODS: Ultrahigh-speed video-microscopy was used to record single laser pulses at 0.2-1.0 J energy lasered with 242 µm glass-core-diameter fibers in contact with whole surgically retrieved urinary stones and hydroxyapatite (HA)-coated glass slides in air and water. Acoustic transients were measured with a hydrophone. Visible-light and infrared photodetectors resolved temporal profiles of visible-light emission and infrared-laser pulses. RESULTS: Temporal profiles of laser pulses showed intensity spikes of various duration and amplitude. The pulses were seen to produce dim light and bright sparks with submicrosecond risetime. The spark produced by the intensity spike at the beginning of laser pulse generated a shock wave in the surrounding liquid. The subsequent sparks were in a vapor bubble and generated no shock waves. Sparks enhanced absorption of laser radiation, indicative of plasma formation and optical breakdown. The occurrence and number of sparks varied even with the same urinary stone. Sparks were consistently observed at laser energy >0.5 J with HA-coated glass slides. The slides broke or cracked by cavitation with sparks in 63 ± 15% of pulses (1.0 J, N = 60). No glass-slide breakage occurred without sparks (1.0 J, N = 500). CONCLUSION: Unappreciated in previous studies, plasma formation with free-running long-pulse holmium:YAG lasers can be an additional physical mechanism of action in laser procedures.
Assuntos
Lasers de Estado Sólido , Litotripsia a Laser , Cálculos Urinários , Humanos , Litotripsia a Laser/métodos , Lasers de Estado Sólido/uso terapêutico , Hólmio , Cálculos Urinários/terapia , ÍtrioRESUMO
Ultra-high-speed video microscopy and numerical modeling were used to assess the dynamics of microbubbles at the surface of urinary stones. Lipid-shell microbubbles designed to accumulate on stone surfaces were driven by bursts of ultrasound in the sub-MHz range with pressure amplitudes on the order of 1 MPa. Microbubbles were observed to undergo repeated cycles of expansion and violent collapse. At maximum expansion, the microbubbles' cross-section resembled an ellipse truncated by the stone. Approximating the bubble shape as an oblate spheroid, this study modeled the collapse by solving the multicomponent Euler equations with a two-dimensional-axisymmetric code with adaptive mesh refinement for fine resolution of the gas-liquid interface. Modeled bubble collapse and high-speed video microscopy showed a distinctive circumferential pinching during the collapse. In the numerical model, this pinching was associated with bidirectional microjetting normal to the rigid surface and toroidal collapse of the bubble. Modeled pressure spikes had amplitudes two-to-three orders of magnitude greater than that of the driving wave. Micro-computed tomography was used to study surface erosion and formation of microcracks from the action of microbubbles. This study suggests that engineered microbubbles enable stone-treatment modalities with driving pressures significantly lower than those required without the microbubbles.
Assuntos
Simulação por Computador , Elasticidade/fisiologia , Microscopia de Vídeo , Cálculos Urinários/fisiopatologia , Acústica , Meios de Contraste/farmacologia , Microbolhas , Microscopia de Vídeo/métodos , Modelos Biológicos , Cálculos Urinários/diagnósticoRESUMO
It has been suggested that bubble-wall velocities cannot exceed the sound speed in the liquid at the bubble wall [K. Yasui, Phys. Rev. E 64, 016310 (2001).10.1103/PhysRevE.64.016310]. Here we show that this upper bound was derived omitting the partial derivatives with respect to time, i.e., assuming that the flow was in the steady state. For collapsing bubbles, however, the steady-flow assumption requires justification, as the partial time derivatives appear to have the same orders of magnitude as the other terms in Euler's and the continuity equations. Furthermore, numerical solutions of the hydrodynamic equations with a hyades hydrocode yielded supersonic velocities in the liquid at and near the collapsing bubble. We also show the spatial distributions of pressure, density, sound speed, and mass flux density around the supersonically collapsing bubble.
RESUMO
A novel treatment modality incorporating calcium-adhering microbubbles has recently entered human clinical trials as a new minimally-invasive approach to treat urinary stones. In this treatment method, lipid-shell gas-core microbubbles can be introduced into the urinary tract through a catheter. Lipid moities with calcium-adherance properties incorporated into the lipid shell facilitate binding to stones. The microbubbles can be excited by an extracorporeal source of quasi-collimated ultrasound. Alternatively, the microbubbles can be excited by an intraluminal source, such as a fiber-optic laser. With either excitation technique, calcium-adhering microbubbles can significantly increase rates of erosion, pitting, and fragmentation of stones. We report here on new experiments using high-speed photography to characterize microbubble expansion and collapse. The bubble geometry observed in the experiments was used as one of the initial shapes for the numerical modeling. The modeling showed that the bubble dynamics strongly depends on bubble shape and stand-off distance. For the experimentally observed shape of microbubbles, the numerical modeling showed that the collapse of the microbubbles was associated with pressure increases of some two-to-three orders of magnitude compared to the excitation source pressures. This in-vitro study provides key insights into the use of microbubbles with calcium-adhering moieties in treatment of urinary stones.
RESUMO
Presented here are observations of the outcomes of the collapses of large single bubbles in H_{2}O and D_{2}O at high ambient pressures. Experiments were carried out in a high-pressure spherical resonator at ambient pressures of up to 30 MPa and acoustic pressures up to 35 MPa. Monitoring of the collapse events and their outcomes was accomplished using multiframe high-speed photography. Among the observations to be presented are the temporal and spatial evolution of light emissions produced by the collapse events, which were observed to last on the order of 30 ns and have time independent radii on the order of 30µm; the production of Rayleigh-Taylor jets which were observed to travel distances of up to 70µm at speeds in excess of 4500 m/s; the entrainment of the light emitting regions in the jets' remnants; the production of spheroidal objects around the collapse points of the bubbles, far from any surface of the resonator; and the traversal and emergence of the Rayleigh-Taylor jets through the spherical objects. These spheroidal objects appear to behave as amorphous solids and form at locations where hydrodynamics predicts pressures in excess of the known transition pressures of water into the high-pressure crystalline ices, Ice-VI and Ice-VII.
RESUMO
Cavitation is usually performed at hydrostatic pressures at or near 0.1 MPa. Higher static pressure produces more intense cavitation, but requires an apparatus that can build high amplitude acoustic waves with rarefactions exceeding the cavitation threshold. The absence of such an apparatus has prevented the achievement of intense acoustic cavitation, hindering research and the development of new applications. Here we describe a new high-pressure spherical resonator system, as well as experimental and modeling results in water and liquid metal (gallium), for cavitation at hydrostatic pressures between 10 and 150 MPa. Our computational data, using HYADES plasma hydrodynamics code, show the formation of dense plasma that, under these conditions, reaches peak pressures of about three to four orders of magnitude greater than the hydrostatic pressure in the bulk liquid and temperatures in the range of 100,000 K. Passive cavitation detection (PCD) data validate both a linear increase in shock wave amplitude and the production of highly intense concentrations of mechanical energy in the collapsing bubbles. High-speed camera observations show the formation of bubble clusters from single bubbles. The increased shock wave amplitude produced by bubble clusters, measured using PCD and fiber optic probe hydrophone, was consistent with current understanding that bubble clusters enable amplification of energy produced.
RESUMO
Cavitation generated by lithotripter shock waves (SWs) in non-degassed water was studied using a 60 frames-per-second camcorder-recording the migration of microbubbles over successive SWs. Lithotripter SWs were produced using a Dornier DoLi-50 electromagnetic lithotripter at 0.5 and 2 Hz pulse repetition frequency (PRF). Cavitation was affected by PRF and by the power level (PL) of the lithotripter. At slow PRF, such as shots fired many seconds apart, cavitation was relatively sparse and bubble clouds flowed in the direction of SW propagation. When PRF was increased, the bubble clouds generated by one SW were amplified by subsequent SWs. Cloud amplification was accompanied by an apparent change in the pattern of bubble migration. Whereas bubbles continued to enter the field of view from the prefocal side, the main bubble cloud remained near the focal point. This was due to a streaming of bubbles opposite to the direction of SW propagation. Increasing the PL grew the cavitation field and enhanced the flow of bubbles opposite to the direction of SW propagation. Stepping up the PL acted to push the broad cloud progressively prefocally (toward the SW source), shifting the position of the plane at which the opposing directional bubble flows collided. (NIH DK43881).
RESUMO
PURPOSE: Conduct a laboratory evaluation of a novel low-pressure, broad focal zone electrohydraulic lithotripter (TRT LG-380). METHODS: Mapping of the acoustic field of the LG-380, along with a Dornier HM3, a Storz Modulith SLX, and a XiXin CS2012 (XX-ES) lithotripter was performed using a fiberoptic hydrophone. A pig model was used to assess renal response to 3000 shockwaves (SW) administered by a multistep power ramping protocol at 60 SW/min, and when animals were treated at the maximum power setting at 120 SW/min. Injury to the kidney was assessed by quantitation of lesion size and routine measures of renal function. RESULTS: SW amplitudes for the LG-380 ranged from (P(+)/P(-)) 7/-1.8 MPa at PL-1 to 21/-4 MPa at PL-11 while focal width measured ~20 mm, wider than the HM3 (8 mm), SLX (2.6 mm), or XX-ES (18 mm). For the LG-380, there was gradual narrowing of the focal width to ~10 mm after 5000 SWs, but this had negligible effect on breakage of model stones, because stones positioned at the periphery of the focal volume (10 mm off-axis) broke nearly as well as stones at the target point. Kidney injury measured less than 0.1% FRV (functional renal volume) for pigs treated using a gradual power ramping protocol at 60 SW/min and when SWs were delivered at maximum power at 120 SW/min. CONCLUSIONS: The LG-380 exhibits the acoustic characteristics of a low-pressure, wide focal zone lithotripter and has the broadest focal width of any lithotripter yet reported. Although there was a gradual narrowing of focal width as the electrode aged, the efficiency of stone breakage was not affected. Because injury to the kidney was minimal when treatment followed either the recommended slow SW-rate multistep ramping protocol or when all SWs were delivered at fast SW-rate using maximum power, this appears to be a relatively safe lithotripter.
Assuntos
Rim/lesões , Litotripsia/efeitos adversos , Litotripsia/instrumentação , Acústica , Animais , Desenho de Equipamento , Feminino , Modelos Animais , SuínosRESUMO
Imaging techniques have been used to capture the temporal and spatial evolution of light emissions from collapsing bubble clouds at high static pressures. Emission events lasting up to 70 ns with peak diameters nearing 1 mm have been observed. Observations of the cloud evolution before and after emission events have been made. Photomultiplier tube monitoring has been employed in conjunction with imaging to study the temporal characteristics of light emission.
RESUMO
UNLABELLED: Study Type--Therapy (case series) Level of Evidence 4. What's known on the subject? and What does the study add? In shock wave lithotripsy air pockets tend to get caught between the therapy head of the lithotripter and the skin of the patient. Defects at the coupling interface hinder the transmission of shock wave energy into the body, reducing the effectiveness of treatment. This in vitro study shows that ineffective coupling not only blocks the transmission of acoustic pulses but also alters the properties of shock waves involved in the mechanisms of stone breakage, with the effect dependent on the size and location of defects at the coupling interface. OBJECTIVE: ⢠To determine how the size and location of coupling defects caught between the therapy head of a lithotripter and the skin of a surrogate patient (i.e. the acoustic window of a test chamber) affect the features of shock waves responsible for stone breakage. MATERIALS AND METHODS: ⢠Model defects were placed in the coupling gel between the therapy head of a Dornier Compact-S electromagnetic lithotripter (Dornier MedTech, Kennesaw, GA, USA) and the Mylar (biaxially oriented polyethylene terephthalate) (DuPont Teijin Films, Chester, VA, USA) window of a water-filled coupling test system. ⢠A fibre-optic probe hydrophone was used to measure acoustic pressures and map the lateral dimensions of the focal zone of the lithotripter. ⢠The effect of coupling conditions on stone breakage was assessed using gypsum model stones. RESULTS: ⢠Stone breakage decreased in proportion to the area of the coupling defect; a centrally located defect blocking only 18% of the transmission area reduced stone breakage by an average of almost 30%. ⢠The effect on stone breakage was greater for defects located on-axis and decreased as the defect was moved laterally; an 18% defect located near the periphery of the coupling window (2.0 cm off-axis) reduced stone breakage by only ~15% compared to when coupling was completely unobstructed. ⢠Defects centred within the coupling window acted to narrow the focal width of the lithotripter; an 8.2% defect reduced the focal width ~30% compared to no obstruction (4.4 mm vs 6.5 mm). ⢠Coupling defects located slightly off centre disrupted the symmetry of the acoustic field; an 18% defect positioned 1.0 cm off-axis shifted the focus of maximum positive pressure ~1.0 mm laterally. ⢠Defects on and off-axis imposed a significant reduction in the energy density of shock waves across the focal zone. CONCLUSIONS: ⢠In addition to blocking the transmission of shock-wave energy, coupling defects also disrupt the properties of shock waves that play a role in stone breakage, including the focal width of the lithotripter and the symmetry of the acoustic field ⢠The effect is dependent on the size and location of defects, with defects near the centre of the coupling window having the greatest effect. ⢠These data emphasize the importance of eliminating air pockets from the coupling interface, particularly defects located near the centre of the coupling window.
Assuntos
Cálculos Renais/terapia , Litotripsia/instrumentação , Modelos Teóricos , Desenho de Equipamento , HumanosRESUMO
PURPOSE: Brushite stones were imaged in vitro and then broken with shock wave lithotripsy to assess whether stone fragility correlates with internal stone structure visible on helical computerized tomography. MATERIALS AND METHODS: A total of 52 brushite calculi were scanned by micro computerized tomography, weighed, hydrated and placed in a radiological phantom. Stones were scanned using a Philips® Brilliance iCT 256 system and images were evaluated for the visibility of internal structural features. The calculi were then treated with shock wave lithotripsy in vitro. The number of shock waves needed to break each stone to completion was recorded. RESULTS: The number of shock waves needed to break each stone normalized to stone weight did not differ by HU value (p = 0.84) or by computerized tomography visible structures that could be identified consistently by all observers (p = 0.053). Stone fragility correlated highly with stone density and brushite content (each p <0.001). Calculi of almost pure brushite required the most shock waves to break. When all observations of computerized tomography visible structures were used for analysis by logistic fit, computerized tomography visible structure predicted increased stone fragility with an overall area under the ROC curve of 0.64. CONCLUSIONS: The shock wave lithotripsy fragility of brushite stones did not correlate with internal structure discernible on helical computerized tomography. However, fragility did correlate with stone density and increasing brushite mineral content, consistent with clinical experience with patients with brushite calculi. Thus, current diagnostic computerized tomography technology does not provide a means to predict when brushite stones will break well using shock wave lithotripsy.
Assuntos
Fosfatos de Cálcio , Cálculos Renais/diagnóstico por imagem , Cálculos Renais/terapia , Litotripsia , Tomografia Computadorizada Espiral , Técnicas In VitroRESUMO
UNLABELLED: What's known on the subject? and What does the study add? Of all the SW lithotriptors manufactured to date, more research studies have been conducted on and more is known about the injury (both description of injury and how to manipulate injury size) produced by the Dornier HM-3 than any other machine. From this information have come suggestions for treatment protocols to reduce shock wave (SW)-induced injury for use in stone clinics. By contrast, much less is known about the injury produced by narrow-focus and high-pressure lithotriptors like the Storz Modulith SLX. In fact, a careful study looking at the morphology of the injury produced by the SLX itself is lacking, as is any study exploring ways to reduce renal injury by manipulating SW delivery variables of this lithotriptor. The present study quantitates the lesion size and describes the morphology of the injury produced by the SLX. In addition, we report that reducing the SW delivery rate, a manoeuvre known to lower injury in the HM-3, does not reduce lesion size in the SLX. OBJECTIVE: ⢠To assess renal injury in a pig model after treatment with a clinical dose of shock waves using a narrow focal zone (≈3 mm) lithotriptor (Modulith SLX, Karl Storz Lithotripsy). MATERIALS AND METHODS: ⢠The left kidney of anaesthetized female pigs were treated with 2000 or 4000 shock waves (SWs) at 120 SWs/min, or 2000 SWs at 60 SWs/min using the Storz SLX. ⢠Measures of renal function (glomerular filtration rate and renal plasma flow) were collected before and 1 h after shock wave lithotripsy (SWL) and the kidneys were harvested for histological analysis and morphometric quantitation of haemorrhage in the renal parenchyma with lesion size expressed as a percentage of functional renal volume (FRV). ⢠A fibre-optic probe hydrophone was used to determine acoustic output and map the focal width of the lithotriptor. ⢠Data for the SLX were compared with data from a previously published study in which pigs of the same age (7-8 weeks) were treated (2000 SWs at 120 or 60 SWs/min) using an unmodified Dornier HM3 lithotriptor. RESULTS: ⢠Treatment with the SLX produced a highly focused lesion running from cortex to medulla and often spanning the full thickness of the kidney. Unlike the diffuse interstitial haemorrhage observed with the HM3, the SLX lesion bore a blood-filled core of near-complete tissue disruption devoid of histologically recognizable kidney structure. ⢠Despite the intensity of tissue destruction at the core of the lesion, measures of lesion size based on macroscopic determination of haemorrhage in the parenchyma were not significantly different from kidneys treated using the HM3 (2000 SWs, 120 SWs/min: SLX, 1.86 ± 0.52% FRV; HM3, 3.93 ± 1.29% FRV). ⢠Doubling the SW dose of the SLX from 2000 to 4000 SWs did not significantly increase lesion size. In addition, slowing the firing rate of the SLX to 60 SWs/min did not reduce the size of the lesion (2.16 ± 0.96% FRV) compared with treatment at 120 SWs/min, as was the case with the HM3 (0.42 ± 0.23% FRV vs 3.93 ± 1.29% FRV). ⢠Renal function fell significantly below baseline in all treated groups but was similar for both lithotriptors. ⢠Focal width of the SLX (≈2.6 mm) was about one-third that of the HM3 (≈8 mm) while peak pressures were higher (SLX at power level 9: P+≈90 MPa, P-≈-12 MPa; HM3 at 24 kV: P+≈46 MPa, P-≈-8 MPa). CONCLUSIONS: ⢠The lesion produced by the SLX (narrow focal width, high acoustic pressure) was a more focused, more intense form of tissue damage than occurs with the HM3. ⢠Slowing the SW rate to 60 SWs/min, a strategy shown to be effective in reducing injury with the HM3, was not protective with the SLX. ⢠These findings suggest that the focal width and acoustic output of a lithotriptor affect the renal response to SWL.
Assuntos
Rim/lesões , Litotripsia/efeitos adversos , Animais , Velocidade do Fluxo Sanguíneo/fisiologia , Pressão Sanguínea/fisiologia , Feminino , Taxa de Filtração Glomerular/fisiologia , Rim/irrigação sanguínea , Rim/fisiologia , Litotripsia/instrumentação , Sus scrofaRESUMO
Lithotripter shock waves (SWs) generated in non-degassed water at 0.5 and 2 Hz pulse repetition frequency (PRF) were characterized using a fiber-optic hydrophone. High-speed imaging captured the inertial growth-collapse-rebound cycle of cavitation bubbles, and continuous recording with a 60 fps camcorder was used to track bubble proliferation over successive SWs. Microbubbles that seeded the generation of bubble clouds formed by the breakup of cavitation jets and by bubble collapse following rebound. Microbubbles that persisted long enough served as cavitation nuclei for subsequent SWs, as such bubble clouds were enhanced at fast PRF. Visual tracking suggests that bubble clouds can originate from single bubbles.
Assuntos
Ondas de Choque de Alta Energia , Litotripsia/instrumentação , Microbolhas , Desenho de Equipamento , Movimento (Física) , Processamento de Sinais Assistido por Computador , Fatores de Tempo , Gravação em Vídeo , ÁguaRESUMO
PURPOSE: Lithotriptors with 2 treatment heads deliver shock waves along separate paths. Firing 1 head and then the other in alternating mode has been suggested as a strategy to treat stones twice as rapidly as with conventional shock wave lithotripsy. Because the shock wave rate is known to have a role in shock wave lithotripsy induced injury, and given that treatment using 2 separate shock wave sources exposes more renal tissue to shock wave energy than treatment with a conventional lithotriptor, we assessed renal trauma in pigs following treatment at rapid rate (240 shock waves per minute and 120 shock waves per minute per head) using a Duet lithotriptor (Direx Medical Systems, Petach Tikva, Israel) fired in alternating mode. MATERIALS AND METHODS: Eight adult female pigs (Hardin Farms, Danville, Indiana) each were treated with sham shock wave lithotripsy or 2,400 shock waves delivered in alternating mode (1,200 shock waves per head, 120 shock waves per minute per head and 240 shock waves per minute overall at a power level of 10) to the lower renal pole. Renal functional parameters, including glomerular filtration rate and effective renal plasma flow, were determined before and 1 hour after shock wave lithotripsy. The kidneys were perfusion fixed in situ and the hemorrhagic lesion was quantified as a percent of functional renal volume. RESULTS: Shock wave treatment resulted in no significant change in renal function and the response was similar to the functional response seen in sham shock wave treated animals. In 6 pigs treated with alternating mode the renal lesion was small at a mean +/- SEM of 0.22% +/- 0.09% of functional renal volume. CONCLUSIONS: Kidney tissue and function were minimally affected by a clinical dose of shock waves delivered in alternating mode (120 shock waves per minute per head and 240 shock waves per minute overall) with a Duet lithotriptor. These observations decrease concern that dual head lithotripsy at a rapid rate is inherently dangerous.
Assuntos
Túbulos Renais/lesões , Litotripsia/efeitos adversos , Litotripsia/instrumentação , Análise de Variância , Animais , Modelos Animais de Doenças , Desenho de Equipamento , Segurança de Equipamentos , Feminino , Imuno-Histoquímica , Cálculos Renais/terapia , Testes de Função Renal , Túbulos Renais/patologia , Imageamento por Ressonância Magnética , Probabilidade , Distribuição Aleatória , Fluxo Plasmático Renal , Sensibilidade e Especificidade , SuínosRESUMO
OBJECTIVE: To determine the mechanism that underlies the effect of shock wave (SW) rate on the performance of clinical lithotripters. MATERIALS AND METHODS: The effect of firing rate on the pressure characteristics of SWs was assessed using a fibre-optic probe hydrophone (FOPH 500, RP Acoustics, Leutenbach, Germany). Shock waves were fired at slow (5-27 SW/min) and fast (100-120 SW/min) rates using a conventional high-pressure lithotriptor (DoLi-50, Dornier MedTech America, Inc., Kennesaw, GA, USA), and a new low-pressure lithotriptor (XX-ES, Xi Xin Medical Instruments Co. Ltd, Suzhou, PRC). A digital camcorder (HDR-HC3, Sony, Japan) was used to record cavitation fields, and an ultrafast multiframe high-speed camera (Imacon 200, DRS Data & Imaging Systems, Inc., Oakland, NJ, USA) was used to follow the evolution of bubbles throughout the cavitation cycle. RESULTS: Firing rate had little effect on the leading positive-pressure phase of the SWs with the DoLi lithotriptor. A slight reduction ( approximately 7%) of peak positive pressure (P+) was detected only in the very dense cavitation fields (approximately 1000 bubbles/cm(3)) generated at the fastest firing rate (120 SW/min) in nondegassed water. The negative pressure of the SWs, on the other hand, was dramatically affected by firing rate. At 120 SW/min the peak negative pressure was reduced by approximately 84%, the duration and area of the negative pressure component was reduced by approximately 80% and approximately 98%, respectively, and the energy density of negative pressure was reduced by >99%. Whereas cavitation bubbles proliferated at fast firing rates, HS-camera images showed the bubbles that persisted between SWs were very small (<10 microm). Similar results were obtained with the XX-ES lithotriptor but only after recognizing a rate-dependent charging artefact with that machine. CONCLUSION: Increasing the firing rate of a lithotriptor can dramatically reduce the negative pressure component of the SWs, while the positive pressure remains virtually unaffected. Cavitation increases as the firing rate is increased but as the bubbles collapse, they break into numerous microbubbles that, because of their very small size, do not pose a barrier to the leading positive pressure of the next SW. These findings begin to explain why stone breakage in SWL becomes less efficient as the firing rate is increased.
Assuntos
Cálculos Renais/terapia , Litotripsia/instrumentação , Acústica , Humanos , Litotripsia/métodos , Litotripsia/normasRESUMO
Previous in vitro studies of acoustic coupling in shock wave lithotripsy (SWL) have shown that air pockets trapped at the surface of the treatment head significantly reduce transmission of shock wave (SW) energy to the focal zone of the lithotripter, reducing the effectiveness of stone breakage. Since there are no reliable means to monitor the quality of coupling during SWL, we looked for a practical protocol to improve how coupling is achieved. In vitro studies were performed using a Dornier DoLi-50 lithotripter. LithoClear gel was used to couple the treatment head to the acoustic window of a clear acrylic test tank. Numerous methods of applying gel were tested including common sense variations of routine protocols typically used with patients. For each method the coverage of air pockets (% defects) was determined using digital imaging. Different coupling regimes were tested for effect on the breakage of gypsum model stones. The quality of acoustic coupling was affected by how the gel was handled--how it was dispensed and applied, and whether the gel was applied only to the treatment head or to both the lithotripter water cushion and the test tank (surrogate patient). Dispensing gel from a squeeze bottle for application by hand created significantly more defects than when a large volume (approximately 250 ml) of gel from the stock jug was applied as a mound to just the treatment head (26.5+/-2.7 vs. 1.2+/-0.5% defects, P<0.001). The efficiency of stone breakage was better when gel was applied from the stock jug compared to application by hand (P<0.006). Poor coupling was substantially improved by using the inflation feature of the water cushion to collapse air pockets, but this strategy was not a substitute for establishing good coupling at the outset. The quality of coupling in shock wave lithotripsy can be improved by minimizing the handling of the coupling medium. Hand application of coupling gel is clearly not the best way to prepare for lithotripsy. Better results can be obtained by delivering lithotripsy gel as a bolus to the treatment head alone, and allowing it to spread upon contact between the treatment head and the skin. These in vitro tests also suggest that the inflation feature of the lithotripter may be useful in reducing defects in coupling.
Assuntos
Acústica , Litotripsia/métodos , Sulfato de Cálcio , Géis , Cálculos Renais/terapia , Modelos BiológicosRESUMO
OBJECTIVE: To assess the renal injury response in a pig model treated with a clinical dose of shock waves (SWs) delivered at a slow rate (27 SW/min) using a novel wide focal zone (18 mm), low acoustic pressure (<20 MPa) electromagnetic lithotripter (Xi Xin-Eisenmenger, XX-ES; Xi Xin Medical Instruments Co. Ltd., Suzhou, PRC). MATERIALS AND METHODS: The left kidneys of anaesthetized female pigs were treated with 1500 SWs from either an unmodified electrohydraulic lithotripter (HM3, Dornier MedTech America, Inc., Kennesaw, GA, USA; 18 kV, 30 SW/min) or the XX-ES (9.3 kV, 27 SW/min). Measures of renal function (glomerular filtration rate, GFR, and renal plasma flow) were collected before and after SW lithotripsy, and kidneys were harvested for histological quantification of vascular haemorrhage, expressed as a percentage of the functional renal volume (FRV). A fibre-optic probe hydrophone was used to characterize the acoustic field, and the breakage of gypsum model stones was used to compare the function of the two lithotripters. RESULTS: Kidneys treated with the XX-ES showed no significant change in renal haemodynamic function and no detectable tissue injury. Pigs treated with the HM3 had a modest decline from baseline ( approximately 20%) in both GFR (P > 0.05) and renal plasma flow (P = 0.064) in the treated kidney, but that was not significantly different from the control group. Although most HM3-treated pigs showed no evidence of renal tissue injury, two had focal injury measuring 0.1% FRV, localized to the renal papillae. The width of the focal zone for the XX-ES was approximately 18 mm and that of the HM3 approximately 8 mm. Peak positive pressures at settings used to treat pigs and break model stones were considerably lower for the XX-ES (17 MPa at 9.3 kV) than for the HM3 (37 MPa at 18 kV). The XX-ES required fewer SWs to break stones to completion than did the HM3, with a mean (sd) of 634 (42) and 831 (43) SWs, respectively (P < 0.01). However, conditions were different for these tests because of differences in physical configuration of the two machines. CONCLUSION: The absence of renal injury with the wide focal zone XX-ES lithotripter operated at low shock pressure and a slow SW rate suggests that this lithotripter would be safe when used at the settings recommended for patient treatment. That the injury was also minimal using the Dornier HM3 lithotripter at a slow SW rate implies that the reduced tissue injury seen with these two machines was because they were operated at a slow SW rate. As recent studies have shown stone breakage to be improved when the focal zone is wider than the stone, a wide focal zone lithotripter operated at low pressure and slow rate has the features necessary to provide better stone breakage with less tissue injury.
Assuntos
Cálculos Renais/terapia , Rim/lesões , Litotripsia , Animais , Feminino , Taxa de Filtração Glomerular/fisiologia , Rim/irrigação sanguínea , Rim/fisiopatologia , Litotripsia/efeitos adversos , Litotripsia/instrumentação , Fluxo Plasmático Renal/fisiologia , SuínosRESUMO
Calcium oxalate monohydrate (COM) stones are often resistant to breakage using shock wave (SW) lithotripsy. It would be useful to identify by computed tomography (CT) those COM stones that are susceptible to SW's. For this study, 47 COM stones (4-10 mm in diameter) were scanned with micro CT to verify composition and also for assessment of heterogeneity (presence of pronounced lobulation, voids, or apatite inclusions) by blinded observers. Stones were then placed in water and scanned using 64-channel helical CT. As with micro CT, heterogeneity was assessed by blinded observers, using high-bone viewing windows. Then stones were broken in a lithotripter (Dornier Doli-50) over 2 mm mesh, and SW's counted. Results showed that classification of stones using micro CT was highly repeatable among observers (kappa = 0.81), and also predictive of stone fragility. Stones graded as homogeneous required 1,874 +/- 821 SW/g for comminution, while stones with visible structure required half as many SW/g, 912 +/- 678. Similarly, when stones were graded by appearance on helical CT, classification was repeatable (kappa = 0.40), and homogeneous stones required more SW's for comminution than did heterogeneous stones (1,702 +/- 993 SW/g, compared to 907 +/- 773). Stone fragility normalized to stone size did not correlate with Hounsfield units (P = 0.85). In conclusion, COM stones of homogeneous structure require almost twice as many SW's to comminute than stones of similar mineral composition that exhibit internal structural features that are visible by CT. This suggests that stone fragility in patients could be predicted using pre-treatment CT imaging. The findings also show that Hounsfield unit values of COM stones did not correlate with stone fragility. Thus, it is stone morphology, rather than X-ray attenuation, which correlates with fragility to SW's in this common stone type.
Assuntos
Oxalato de Cálcio , Cálculos Renais/diagnóstico por imagem , Litotripsia , Fenômenos Biomecânicos , Humanos , Cálculos Renais/química , Tomografia Computadorizada EspiralRESUMO
OBJECTIVE: To assess the effect of dual-head lithotripsy on renal function and morphology in a pig model of shockwave (SW) injury, as lithotripters with two shock heads are now available for treating patients, but little information is available with which to judge the safety of treatment with dual pulses. MATERIALS AND METHODS: A dual-head electrohydraulic lithotripter (Duet, Direx Corp., Natick, MA, USA) was used to treat the lower renal pole of anaesthetized pigs with a clinical dose of SWs (2400 dual SWs; 10 kidneys) delivered in synchronous mode, i.e. both heads fired simultaneously. For comparison, pigs were treated with either 2400 SWs (12 kidneys) or 4800 SWs (eight) with a conventional electrohydraulic lithotripter (HM3, Dornier, Wessling, Germany). RESULTS: Dual-pulse SW treatment with the Duet lithotripter caused a decline in the mean (sd) glomerular filtration rate (GFR) of 4.1 (1.9) mL/min, with a trend for the effective renal plasma flow (RPF), at 31 (19) mL/min, to also decrease. These changes in renal haemodynamics were similar to the decreases in GFR and RPF in response to treatment with the HM3 lithotripter with 2400 SWs, at 4.8 (0.8) and 32 (10) mL/min, respectively, or 4800 SWs, at 5.4 (1.0) and 68 (14) mL/min, respectively. Linear association analysis showed that the functional response to dual-pulse SWs was more variable than with conventional SWs. Morphological quantification of kidney damage (expressed as a percentage of functional renal volume, FRV) showed that tissue injury with 2400 paired SWs with the Duet, at 0.96 (0.39)% FRV, was similar to injury produced by either 2400 single SWs, at 1.08 (0.38)% FRV, or 4800 single SWs, at 2.71 (1.02)% FRV, with the HM3. However, morphological damage was less consistent with the Duet (measurable in only five of eight kidneys) than that with the HM3 (measurable in all 12 kidneys). Acoustic output and the timing of dual SWs in synchronous mode increased in variability as the electrodes aged, affecting the amplitude and targeting of focal pressures. CONCLUSION: With the caveat that variability in the timing of dual SWs will unpredictably alter the distribution of SW energy within the kidney, this study shows that a clinical dose of dual-head SWs delivered in synchronous mode elicits a renal response similar to, but more variable than, that with a clinical dose of SWs from a conventional electrohydraulic lithotripter.
Assuntos
Taxa de Filtração Glomerular/fisiologia , Rim/lesões , Litotripsia/efeitos adversos , Fluxo Plasmático Renal/fisiologia , Animais , Feminino , Rim/fisiopatologia , Modelos Lineares , Litotripsia/instrumentação , SuínosRESUMO
PURPOSE: Current lithotriptors use a dry treatment head that must be coupled to the patient with gel or oil. We determined how the quality of coupling affects stone breakage under conditions that simulated patient treatment. MATERIALS AND METHODS: Experiments were performed with a Dornier (DoLi-50 electromagnetic lithotriptor. The test tank had a clear Mylar membrane for coupling with the treatment head water cushion. Thus, air pockets trapped at the coupling interface could be photographed for quantitation. Coupling efficiency was assessed using a fiberoptic hydrophone and different coupling regimes were tested for the effect on gypsum stone breakage. RESULTS: The quality of coupling was variable with air pockets covering 1.5% to 19% of the coupling area, resulting in a mean decrease in shock wave amplitude of approximately 20%. Breaking and reestablishing contact, as when a patient is repositioned during treatment, decreased acoustic pressure almost 32%, representing a 57% decrease in acoustic energy transmission. Stone breakage was also decreased when air was trapped in coupling and only 2% coverage by air pockets decreased stone breakage by 20% to 40%. CONCLUSIONS: These in vitro results suggest that coupling in lithotripsy can pose a significant barrier to the transmission of shock wave energy to the patient. Stone breakage was sensitive to air pockets at the coupling interface. Recoupling was particularly disruptive, suggesting that repositioning the patient could substantially degrade coupling quality. It seems reasonable that variability in the quality of coupling could contribute to variability in clinical outcomes.