Magnetized chitosan hydrogel and silk fibroin, reinforced with PVA: a novel nanobiocomposite for biomedical and hyperthermia applications.

Herein, a multifunctional nanobiocomposite was designed for biological application, amongst which hyperthermia cancer therapy application was specifically investigated. This nanobiocomposite was fabricated based on chitosan hydrogel (CS), silk fibroin (SF), water-soluble polymer polyvinyl alcohol (PVA) and iron oxide magnetic nanoparticles (Fe3O4 MNPs). CS and SF as natural compounds were used to improve the biocompatibility, biodegradability, adhesion and cell growth properties of the nanobiocomposite that can prepare this nanocomposite for the other biological applications such as wound healing and tissue engineering. Since the mechanical properties are very important in biological applications, PVA polymer was used to increase the mechanical properties of the prepared nanobiocomposite. All components of this nanobiocomposite have good dispersion in water due to the presence of hydrophilic groups such as NH2, OH, and COOH, which is one of the effective factors in increasing the efficiency of hyperthermia cancer therapy. The structural analyzes of the hybrid nanobiocomposite were determined by FT-IR, XRD, EDX, FE-SEM, TGA and VSM. Biological studies such as MTT and hemolysis testing proved that it is hemocompatible and non-toxic for healthy cells. Furthermore, it can cause the death of cancer cells to some extent (20.23%). The ability of the nanobiocomposites in hyperthermia cancer therapy was evaluated. Also, the results showed that it can be introduced as an excellent candidate for hyperthermia cancer therapy.

A novel magnetic nanocomposite based on alginate-tannic acid hydrogel embedded with silk fibroin with biological activity and hyperthermia application.

In the current study, sodium alginate (SA) and tannic acid (TA), in the presence of calcium chloride as a cross-linker, were used to fabricate a nanocomposite scaffold. With the addition of silk fibroin (SF), the strength of the synthesized composite was increased. Fe3O4 magnetic nanoparticles (MNPs) led to the usage of this magnetic nanocomposite in hyperthermia applications. Various properties of this scaffold were investigated by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR), energy dispersive X-Ray (EDX), Vibrating- sample magnetometer (VSM). A hemolytic assay of this magnetic nanocomposite demonstrated that about 100 % of red blood cells (RBCs) survived at a concentration of 2 mg/ml, proving this scaffold is hemocompatible. Furthermore, an MTT assay was utilized to assess the cytotoxicity of the synthesized magnetic nanocomposite. Finally, the hyperthermia behavior of the fabricated magnetic nanocomposite was evaluated, and the specific absorption rate (SAR) was 73.53 W/g. The proposed nanocomposite is a good candidate for wound dressing applications in future studies.