RESUMO
Two novel endophytic bacterial strains, designated KSS8T and KSS12T, were isolated from the stems and roots of sugarcane, respectively, collected in Nakhon Ratchasima, Thailand. They were Gram-stain-negative, aerobic, and rod-shaped. The strain KSS8T was a motile bacterium with a subpolar flagellum, while the strain KSS12T was non-motile. Strains KSS8T and KSS12T were closely related to Lichenicola cladoniae PAMC 26569T (97.3 and 95.6 %, respectively) and Lichenicoccus roseus KEBCLARHB70RT (97.2 and 95.8 %, respectively) based on the similarity on their 16S rRNA gene sequence. This similarity corresponded to their phylogenomic positions within the evolutionary radiation of the family Acetobacteraceae. The average nucleotide identities and digital DNA-DNA hybridization values between the genome sequences of the two strains and other genera were significantly lower than the defined threshold values of 95-96 % and 70 %, respectively, which are used for the delineation of prokaryotic species. Both strains contained summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, C19:0 cyclo ω8c, C18:0, and C18:1 2OH as the predominant cellular fatty acids, but C18:3 ω6c (6, 9, 12) were found only in strain KSS12T. Based on phenotypic, chemotaxonomic, phylogenetic, and genomic analyses, these strains clearly represented two novel genera within the family Acetobacteraceae, for which the name Endosaccharibacter gen. nov., with the type species Endosaccharibacter trunci sp. nov. (type strain, KSS8T = TBRC 14669T = NBRC 115232T = KCTC 92115T = LMG 32414T) and the name Rhizosacchari bacter gen. nov., with the type species Rhizosaccharibacter radicis sp. nov. (type strain, KSS12T = TBRC 13066T = NBRC 114898T = KCTC 82433T = LMG 32137T) are proposed.
RESUMO
The aim of the present study was to isolate and identify lactic acid bacteria (LAB) from fermented juice of tropical crops such as Napier grass, Ruzi grass, Purple guinea grass, Stylo legume, and Leucaena and their application to improve the quality of tropical crop silage. Fifteen strains of LAB were isolated. The LAB strains were Gram-positive and catalase-negative bacteria and could be divided into three groups, i.e., Pediococcus pentosaceus, Lactiplantibacillus (para)plantarum, and Limosilactobacillus fermentum according to the biochemical API 50CH test. Based on the analysis of 16S rRNA sequence, the strains isolated in the group L. (para)plantarum were distinguished. Two isolates (N3 and G4) were identified as Lactiplantibacillus plantarum. Three isolates (St1, St2, and St3) were identified as L. paraplantarum. In addition, the identification of other isolates was confirmed in the group P. pentosaceus (R1, R4, R5, R8, R11, and L1) and the group L. fermentum (N4, G6, G7, and N4). All selected strains were able to grow at 50°C. All LAB strains showed antimicrobial activity against Escherichia coli ATCC 25922, Shigella sonnei ATCC 25931, Pseudomonas aeruginosa ATCC 27853, and Bacillus cereus ATCC 11778. Four selected LAB strains (St1, St3, N4, and R4) were tested for their capacity to successfully ensile Stylo legume (Stylosanthes guianensis CIAT184). Stylo silages treated with LAB were well preserved, the NH3-N and butyric acid contents were lower, and the lactic acid content was higher than those in the control (p < 0.05). The acetic acid content was the highest in R4-treated silage among the treatments (p < 0.05). The crude protein (CP) content of St1-silage was significantly (p < 0.05) higher than the others. The inoculation of thermotolerant LAB selected from fermented juice of epiphytic lactic acid bacteria (FJLB) was found to be highly instrumental to obtain well-preserved silage from the Stylo legume.
RESUMO
The use of endophytic bacteria in agriculture provides an effective way of improving crop yield and significantly reducing chemical usage, such as fungicides. This research was conducted to explore endophytic bacteria with plant growth promotion (PGP) and antifungal activities against Fusarium moniliforme AIT01. In this study, we obtained 52 isolates of endophytic bacteria associated with the roots and stems of sugarcane from Nakhon Ratchasima province, Thailand. In vitro antagonistic activity test showed that 14 out of 52 isolates had antagonistic activity against the fungal pathogen F. moniliforme AIT01. These antagonistic endophytic bacteria were identified as belonging to six different species as follows: Nguyenibacter vanlangensis, Acidomonas methanolica, Asaia bogorensis, Tanticharoenia aidae, Burkholderia gladioli and Bacillus altitudinis based on phenotypic characteristics, along with phylogenetic analysis of their 16S rRNA gene sequences. Seven isolates effectively inhibited F. moniliforme AIT01 mycelial growth by up to 40%. The volatile compounds of six isolates reduced the growth of F. moniliforme AIT01 by over 23%. Moreover, riceberry rice seedlings previously treated with B. gladioli CP28 were found to strongly reduce infection with phytopathogen by 80% in comparison to the non-treated control. Furthermore, the isolates also showed relevant PGP features, including ammonia production, zinc and phosphate solubilisation, auxin and siderophore biosynthesis. These results demonstrated that the tested endophytic bacteria could be successfully utilised as a source of PGP and biocontrol agent to manage diseases caused by F. moniliforme.
