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1.
Artigo em Inglês | MEDLINE | ID: mdl-38928976

RESUMO

A recent study conducted in Khon Kaen Province, Thailand, evaluated the effectiveness of a technology-assisted intervention aimed at improving water quality and addressing related health issues in communities around key water bodies. The intervention targeted health concerns associated with water contamination, including chronic kidney diseases, skin conditions, hypertension, and neurological symptoms. The study included water quality assessments and health evaluations of 586 residents and implemented a Learning Innovation Platform (LIP) across 13 communities. Results showed significant improvements in the community, including a decrease in hypertension and skin-related health issues, as well as enhanced community awareness and proficiency in implementing simple water quality assessments and treatment. The study demonstrated the value of a comprehensive, technology-driven community approach, effectively enhancing water quality and health outcomes, and promoting greater community awareness and self-sufficiency in managing environmental health risks.


Assuntos
Qualidade da Água , Tailândia , Humanos , Feminino , Masculino , Adulto , Poluição da Água , Pessoa de Meia-Idade , Dermatopatias/terapia
2.
Spectrochim Acta A Mol Biomol Spectrosc ; 317: 124429, 2024 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-38754203

RESUMO

Mercury ion is one of the most harmful metal ions with significant hazards to the environment and human health. Thus, the development of innovative, sensitive, and selective sensors to help address the detrimental impacts of heavy metal contamination is necessary. In this work, we present three new chemosensors based on the deprotection reaction of the thioacetal group for distinguishing Hg2+ in environmental samples. These chemosensors show good photophysical properties with high quantum yield in aqueous medium. These prepared chemosensors were employed as fluorometric sensors for the determination of Hg2+ through the quenching of fluorescence emission due to the Hg2+-induced hydrolysis of the thioacetal to the aldehyde group. In the presence of Hg2+, chemosensors showed an emissive color transformation from blue fluorescence to non-fluorescence under UV light, which was readily seen by the visual eye. These chemosensors also exhibited highly distinctive selectivity toward Hg2+ over other interfering metal ions, with detection limits of 1.1 ppb, 13.4 ppb, and 12.7 ppb. Moreover, the practical applicability of chemosensor was successfully demonstrated in real water samples and herb extract samples.


Assuntos
Corantes Fluorescentes , Mercúrio , Espectrometria de Fluorescência , Poluentes Químicos da Água , Mercúrio/análise , Espectrometria de Fluorescência/métodos , Corantes Fluorescentes/química , Humanos , Poluentes Químicos da Água/análise , Limite de Detecção
3.
Org Biomol Chem ; 22(3): 506-512, 2024 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-38111346

RESUMO

Combination of cysteine-containing peptides with electrophiles provides efficient access to cyclo-organopeptides. However, there are no routes to intrinsically fluorescent cyclo-organopeptides containing robust, brilliant fluorophores emitting at wavelengths longer than cellular autofluorescence. We show such fluorescent cyclo-organopeptides can be made via SNAr reactions of cysteine-containing peptides with a BODIPY system. Seven compounds of this type were prepared to test as probes; six contained peptide sequences corresponding to loop regions in brain-derived neurotrophic factor and neurotrophic factor 4 (BDNF and NT-4) which bind tropomyocin receptor kinase B (TrkB). Cellular assays in serum-free media indicated two of the six key compounds induced survival of HEK293 cells stably transfected with TrkB whereas a control did not. The two compounds inducing cell survival bound TrkB on those cells (Kd ∼40 and 47 nM), illustrating how intrinsically fluorescent cyclo-organopeptides can be assayed for quantifiable binding to surface receptors in cell membrane environments.


Assuntos
Cisteína , Humanos , Células HEK293 , Membrana Celular
4.
Sci Rep ; 12(1): 4173, 2022 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-35264603

RESUMO

To improve the potency of Heptamethine cyanines (Hcyanines) in cancer research, we designed and synthesized two novel Hcyanines based theranostic probes, IR794-Morph and IR794-Morph-Mpip, to enhance cancer cell internalization and targeting. In acidic conditions that resemble to tumour environment, both IR794 derivatives exhibited broad NIR absorption band (704‒794 nm) and fluorescence emission (798‒828 nm) that is suitable for deep seated tumour imaging. Moreover, in vitro study revealed that IR794-Morph-Mpip exhibited better cancer targetability towards various cancer cell lines under physiological and slightly acidic conditions compared to normal cells. IR794-Morph-Mpip was fast internalized into the cancer cells within the first 5 min and mostly localized in lysosomes and mitochondria. In addition, the internalized signal was brighter when the cells were in the hypoxic environment. Furthermore, cellular uptake mechanism of both IR794 dyes, investigated via flow cytometry, revealed that endocytosis through OATPs receptors and clathrin-mediated endocytosis were the main routes. Moreover, IR794-Morph-Mpip, displayed anti-cancer activity towards all tested cancer cell types with IC50 below 7 µM (at 6 h incubation), which is approximately three times lower than that of the normal cells. Therefore, increasing protonated cites in tumour environment of Hcyanines together with incorporating morpholine in the molecule can enhance structure-inherent targeting of these dyes.


Assuntos
Neoplasias , Quinolinas , Fluorescência , Corantes Fluorescentes/química , Humanos , Morfolinas/farmacologia
5.
J Hazard Mater ; 409: 124487, 2021 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-33199148

RESUMO

Cadmium highly toxic and hazardous, and it can adversely affect human health leading to serious disorders. Herein, a water-soluble near-infrared sensor based on aza-BODIPY (1) was developed for dual determination of Cd2+ in environmental and biological media. This sensor exhibited color change from colorless to green along with a fluorescence enhancement in the near-infrared (NIR) region via photoinduced electron transfer (PET) after complexation with Cd2+. Sensor 1 can be employed in aqueous media at physiological pH for quantitative monitoring. It shows rapid response with high sensitivity (detection limit of 2.8 ppb; linear correlation over [Cd2+] 1.33 - 6.67 µM) and selectivity over potentially interfering ions. NIR sensor 1 can be used to determine [Cd2+] in living cells and environmental samples.


Assuntos
Cádmio , Corantes Fluorescentes , Compostos de Boro , Cádmio/toxicidade , Humanos , Espectrometria de Fluorescência
6.
Chempluschem ; 84(3): 252-259, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-31950759

RESUMO

A near-infrared chemodosimeter based on an aza-BODIPY dye was designed and synthesized. The sensor contains isothiocyanate groups for cyanide ion sensing. The sensing function was illustrated via the fluorescence changes in near-infrared frequencies as well as chromogenic changes which could be easily visualized with a detection limit of 19 ppb. The sensor provides high selectivity to CN- and discriminates other anions such as CH3 COO- , HPO4- , HSO4- , ClO3- , CO32- , SO42- , NO3- , Cl- , F- , Br- , I- , and phenylalanine (Phe) in 50 % PBS buffer/acetonitrile at physiological pH. The potential of the sensor for CN- detection in both aqueous buffer solutions and living cells imaging was demonstrated.


Assuntos
Compostos de Boro/química , Cianetos/análise , Corantes Fluorescentes/química , Isotiocianatos/química , Animais , Compostos de Boro/síntese química , Compostos de Boro/toxicidade , Linhagem Celular , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/toxicidade , Isotiocianatos/síntese química , Isotiocianatos/toxicidade , Limite de Detecção , Camundongos , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos , Espectrometria de Fluorescência/métodos , Poluentes Químicos da Água/análise
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