RESUMO
We evaluated the performance of the Emit tox benzodiazepine and barbiturate assays (Dade Behring) and fluorescence polarisation immunoassay (FPIA) (Abbott) for use with serum determinations in preliminary therapeutic drug monitoring or acute drug intoxication detection. Performance, as indicated by CVs, of the Emit tox benzodiazepine and barbiturate assays and FPIA showed that both immunochemical techniques are precise and have good reproducibility. For within-run studies, results from benzodiazepine determinations showed maximum CV values of 1.91% for the Emit method and 2.65% for FPIA; results from barbiturate determinations showed maximum CV values of 2.01% for the Emit method and 1.89% for FPIA. For between-run studies, results from benzodiazepine determinations showed maximum CV values of 1.79% for the Emit method and 1.12% for FPIA; results from barbiturate determinations showed maximum CV values of 2.09% for the Emit method and 2.02% for FPIA.
Assuntos
Barbitúricos/sangue , Benzodiazepinas/sangue , Técnica de Imunoensaio Enzimático de Multiplicação , Imunoensaio de Fluorescência por Polarização/métodos , Barbitúricos/intoxicação , Benzodiazepinas/intoxicação , Calibragem , Cromatografia Líquida de Alta Pressão , Humanos , Kit de Reagentes para Diagnóstico , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Pesticides are widely used to control any pest, including vectors of diseases, unwanted species of plants or animals. They are ubiquitous contaninants of our environment and have been found in water, vegetables, human and animal tissues. Acute or chronic intoxications are well documented. Some pesticides seem to have additive or synergistic effects of oestrogenic compounds, influencing the endocrine functions in several animal species. Moreover, pesticides can induce mutagenesis, carcinogenesis and immunotoxicity.
Assuntos
Exposição Ambiental/efeitos adversos , Saúde Ambiental , Nível de Saúde , Resíduos de Praguicidas/efeitos adversos , Resíduos de Praguicidas/intoxicação , Animais , Sinergismo Farmacológico , Estradiol/fisiologia , HumanosRESUMO
Immunotoxicity of drugs represents an important problem for 10 to 12% of the overall population. There are 4 types immunotoxicological manifestations of drugs: immediate hypersensitivity, cytotoxic reactions, immune complexes reactions and delayed-type hypersensitivity. The most frequently concerned drugs are penicillins, cephalosporins, anesthetics (type I), erythromycin estolate, nitrofurantoin, sulfonamids, antitubercular agents (type II), quinidine, ticarcillin, valproate, pyramidon (type III) and finally for type IV, topical drugs (antihistaminic products, oestradiol, s.c. heparin).
