RESUMO
The passing and optic-density parameters registered by a photometric device were estimated, on the basis of a simple modeled system, with respect to the ratio between the absorption band width and the heterogeneous radiation degree. The impacts of heterogeneous radiation on the validity of the Bueguer'-Lambert's-Baire's law were elucidated.
Assuntos
Espectrofotometria/métodos , Algoritmos , Espectrofotometria/instrumentação , Espectrofotometria Infravermelho/instrumentação , Espectrofotometria Infravermelho/métodos , Espectrofotometria Ultravioleta/instrumentação , Espectrofotometria Ultravioleta/métodosRESUMO
The potentialities of photometry in the ultraviolet, visible, and infrared spectra are compared. Errors associated with neglect of the Booger-Lambert-Ber law, nonmonochromic light, inconstant length of optic route, and non-optimal choice of optic density range are analyzed.
Assuntos
Técnicas de Laboratório Clínico , Espectrofotometria/métodos , Espectroscopia de Luz Próxima ao Infravermelho , Humanos , Espectrofotometria UltravioletaRESUMO
The effect on the liver phospholipid composition of 1-methyl-4-phenyl -1,2,3,6-tetrahydropyridine (MPTP) and liposomes containing L-DOPA and those devoid of it was studied in experiments on C57B1/6 mice. The content of separate phospholipid fractions was expressed in percentage of lipid phosphorus, in nmol lipid phosphorus per 1 mg tissue, and in nmol lipid phosphorus per 1 mg protein. It was found that the content of phosphatidylinositide and phosphatidylethanolamine changed under the effect of MPTP. Injection of liposomes stabilized the level of these phospholipids. The concentration of phosphatidylethanolamine was not restored in animals injected liposomes devoid of L-DOPA. The main changes occurring under the effect of liposomes took place in the sphyngomyelin and phosphatidylcholine fractions.