RESUMO
Two variants (direct and indirect) of enzyme linked immunosorbent assay (ELISA) of phenobarbital are compared. Both techniques were developed on the basis of the same monoclonal antibodies, and horse radish peroxidase was used as the label in both cases. When microtitration plates are used as the solid phase, indirect ELISA, in which phenobarbital of the sample competes with phenobarbital sorbed on plates in the form of a conjugate with protein for the binding with peroxidase-labeled antiphenobarbital antibodies, is preferable. In indirect ELISA, the sample volume was 5 microliters, the time of assay was 40 min, the variability coefficient was < 8%.
Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Fenobarbital/análise , Animais , Anticorpos Monoclonais/imunologia , Peroxidase do Rábano Silvestre , Camundongos , Fenobarbital/imunologiaRESUMO
On mice immunized with ram erythrocytes it was shown that caffeine and theophylline (15 to 240 mg/kg thrice at different time intervals with respect to the antigenic stimulus) possess identical immunotropic activity. High doses of methylxanthines injected a few days before immunization increased and being administered during immunization decreased the indices of humoral immunity. Low doses of the drugs stimulated immunity but only when administered at the time close to the antigenic stimulus application.
Assuntos
3',5'-AMP Cíclico Fosfodiesterases/antagonistas & inibidores , Cafeína/imunologia , Teofilina/imunologia , Animais , Relação Dose-Resposta a Droga , Eritrócitos/imunologia , Feminino , Hemaglutininas/análise , Proteínas Hemolisinas/análise , Imunização , Camundongos , Baço/efeitos dos fármacos , Baço/imunologia , Fatores de Tempo , Xantinas/imunologiaRESUMO
It was shown in experiments on mice that the chemically inert compound perfluortributylamine has sufficiently expressed immunopharmacological activity. Its intraperitoneal administration causes a drop in the immune response of mice, immunized with sheep erythrocytes. These animals had a marked durable resistance to the soporific action of hexenal.