1.
J Proteome Res
; 6(8): 3224-31, 2007 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-17629318
RESUMO
S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis.